Oligonucleotides and analogs labeled with energy transfer dyes

• US 7,388,092 B2
• Filed: 12/28/2006
• Issued: 06/17/2008
• Est. Priority Date: 05/03/1996
• Status: Expired due to Fees

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Applera Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Lee, Linda G., Spurgeon, Sandra L., Rosenblum, Barnett
• Primary Examiner(s): Riley; Jezia
• Application Number:
  US11/617,665
• Publication Number:
  US 20070161027A1
• Time in Patent Office: 537 Days
• Field of Search: 536 243- 2433, 536/26.6, 435/6
• US Class Current: 536/24.3
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C07H 21/04 with deoxyribosyl as saccharide radical
• CPC Class Codes: C09B 11/24 Phthaleins containing amino groups ; Phthalanes; Fluoranes; Phthalides; Rhodamine dyes; Phthaleins having heterocyclic aryl rings; Lactone or lactame forms of triarylmethane dyes
• CPC Class Codes: C12Q 1/68 involving nucleic acids
• CPC Class Codes: C12Q 1/6818 involving interaction of two or more labels, e.g. resonant energy transfer
• CPC Class Codes: G01N 33/00 Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00

Abstract

Novel linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye are provided. These linkers faciliate the efficient transfer of energy between a donor and acceptor dye in an energy transfer dye. One of these linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye has the general structure R₂₁Z₁C(O)R₂₂R₂₈ where R₂₁ is a C_1-5 alkyl attached to the donor dye, C(O) is a carbonyl group, Z₁ is either NH, sulfur or oxygen, R₂₂ is a substituent which includes an alkene, diene, alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure which is attached to the carbonyl carbon, and R₂₈ includes a functional group which attaches the linker to the acceptor dye.

Claims

1. A fluorescently labeled reagent comprising an oligonucleotide or oligonuclotide analog and an energy transfer dye, wherein energy transfer dye is covalently linked to the oligonucleotide or oligonucleotide analog and comprises:

a xanthene donor dye capable of absorbing light at a first wavelength and emitting excitation energy in response thereto;

a 4,7-dichlororhodamine acceptor dye capable of absorbing the excitation energy emitted by the donor dye and fluorescing at a second wavelength in response thereto; and

a non-nucleosidic linker linking the xanthene donor dye to the 4′

-, 5- or 6-position of the 4,7-dichlororhodamine acceptor dye.

2. The fluorescently labeled reagent of claim 1 in which the linker has a backbone that is less than 9 atoms in length.

3. The fluorescently labeled reagent of claim 1 in which the linker comprises a functional group selected from an alkene, a diene, an alkyne, a five membered ring having at least one unsaturated bond, a six membered ring having at least one unsaturated bond and a fused ring structure.

4. The fluorescently labeled reagent of claim 1 in which the energy transfer dye is covalently linked to the 5′

-terminus of the oligonucleotide or oligonucleotide analog.

5. The fluorescently labeled reagent of claim 1 in which the energy transfer dye is covalently linked to the 3′

-terminus of the oligonucleotide or oligonucleotide analog.

6. The fluorescently labeled reagent of claim 1 in which the energy transfer dye is covalently linked to a base moiety of the oligonucleotide or oligonucleotide analog.

7. The fluorescently labeled reagent of claim 1 in which the energy transfer dye is covalently linked to the base moiety by way of an acetylenic amido or alkenoic amido linkage.

8. The fluorescently labeled reagent of claim 7 in which the acetylenic amido or alkenoic amido linkage is selected from —

C≡

C—

CH₂—

NH—

C(O)—

, 3-amino-1-propyn-1-yl, —

C≡

C—

CH₂—

NH—

C(O)—

(CH₂)₅—

C(O)—

, —

C═

CH—

C(O)—

NH—

(CH₂)₅—

NH—

C(O)— and

—

C≡

C—

CH₂—

O—

CH₂—

CH₂-NR—

, where R is hydrogen, a protecting group or alkyl.

9. The fluorescently labeled reagent of claim 1 in which the xanthene donor dye is a fluorescein dye.

10. The fluorescently labeled reagent of claim 9 in which the non-nucleosidic linker links the 4′

-position of the fluorescein donor dye to the 5- or 6-position of the 4,7-dichlororhodamine acceptor dye.

11. The fluorescently labeled reagent of claim 10 in which energy transfer dye is covalently linked to the oligonucleotide or oligonucleotide analog via the 5- or 6-position of the fluorescein donor dye or the 4′

-position of the 4,7-dichlororhodamine acceptor dye.

12. The fluorescently labeled reagent of claim 10 in which the 4,7-dichlororhodamine acceptor dye comprises structure (I):

13. The fluorescently labeled reagent of claim 10 in which the fluorescein donor dye comprises structure (II):

14. The fluorescently labeled reagent of claim 10 in which energy transfer dye comprises structure (IV):

15. The fluorescently labeled reagent of claim 14 in which X¹ is carboxylate.

16. The fluorescently labeled reagent of claim 15 in which R¹, R², R³, R⁴, R⁵, R⁶, R⁷, R⁸, R⁹, R¹⁰ and X³ are each hydrogen.

17. The fluorescently labeled reagent of claim 15 in which R¹, R³, R⁵, R⁷, R⁸, R¹⁰ and X³ are each hydrogen;

R² and R⁴ are each ethyl; and

R⁶ and R⁹ are each methyl.

18. The fluorescently labeled reagent of claim 15 in which R¹, R², R³ and R⁴ are each methyl;

and R⁵, R⁶, R⁷, R⁸, R⁹, R¹⁰ and X³ are each hydrogen.

19. The fluorescently labeled reagent of claim 15 in which R¹ and R⁵, R² and R⁶, R³ and R⁸ and R⁴ and R⁹ are taken together with the atoms to which they are bonded to form six-membered rings;

and R⁷, R¹⁰ and X³ are each hydrogen.

20. The fluorescently labeled reagent of claim 15 in which R¹, R³, R⁴, R⁵, R⁷, R⁸, R⁹, R¹⁰ and X³ are each hydrogen;

R² is ethyl; and

R⁶ is methyl.

21. The fluorescently labeled reagent of claim 15 in which R¹, R⁵, R⁷, R⁸, R⁹, R¹⁰ and X³ are each hydrogen;

R³, R⁴ and R⁶ are each methyl; and

R² is ethyl.

22. The fluorescently labeled reagent of claim 15 in which R⁵, R⁶, R⁷, R¹⁰ and X³ are each hydrogen;

R¹ and R² are each methyl; and

R³ and R⁸ and R⁴ and R⁹ are taken together with the atoms to which they are bonded to form six-membered rings.

23. The fluorescently labeled reagent of claim 15 in which R¹, R², R⁵, R⁶, R⁷, R¹⁰ and X³ are each hydrogen;

and R³ and R⁸ and R⁴ and R⁹ are taken together with the atoms to which they are bonded to form six-membered rings.

24. The fluorescently labeled reagent of claim 15 in which R¹, R⁵, R⁶, R⁷, R¹⁰ and X³ are each hydrogen;

R² is ethyl; and

R³ and R⁸ and R⁴ and R⁹ are taken together with the atoms to which they are bonded to form six-membered rings.

25. The fluorescently labeled reagent of claim 15 in which R¹, R², R⁵, R⁶, R⁷, R⁸, R⁹, R¹⁰ and X³ are each hydrogen;

and R³ and R⁴ are each methyl.