Prefilter system for biological systems

• US 7,390,403 B2
• Filed: 02/23/2005
• Issued: 06/24/2008
• Est. Priority Date: 03/19/2004
• Status: Active Grant

• Current Assignee: EMD Millipore Corporation (Merck & Co., Inc.)
• Original Assignee: Millipore Corporation
• Inventors: Siwak, Martin
• Primary Examiner(s): Therkorn; Ernest G
• Application Number:
  US11/063,477
• Publication Number:
  US 20050205489A1
• Time in Patent Office: 1,217 Days
• Field of Search: 210/348, 210/767, 210/788, 210/806, 210/198.2, 210/304, 210/330, 210/331, 210/502.1, 210/512.1
• US Class Current: 210/198.2
• CPC Class Codes: B01D 15/125 Pre-filtration
• CPC Class Codes: B01D 15/3804 Affinity chromatography
• CPC Class Codes: B01D 15/3809 of the antigen-antibody type, e.g. protein A, G, L chromatography
• CPC Class Codes: B01D 2239/0407 comprising particulate additives, e.g. adsorbents apparatus incorporating gas filtering material B01D46/0036
• CPC Class Codes: B01D 2239/0428 Rendering the filter material hydrophobic
• CPC Class Codes: B01D 2239/065 More than one layer present in the filtering material apparatus incorporating such gas filtering material B01D2275/10
• CPC Class Codes: B01D 2239/0654 Support layers
• CPC Class Codes: B01D 2239/0695 Wound layers apparatus incorporating such gas filtering material B01D2275/105
• CPC Class Codes: B01D 2311/04 Specific process operations in the feed stream; Feed pretreatment
• CPC Class Codes: B01D 2311/2626 Absorption or adsorption
• CPC Class Codes: B01D 2311/2649 Filtration
• CPC Class Codes: B01D 29/41 mounted transversely on the tube
• CPC Class Codes: B01D 39/083 of organic material
• CPC Class Codes: B01D 39/086 of inorganic material
• CPC Class Codes: B01D 39/1607 the material being fibrous B01D39/18 takes precedence
• CPC Class Codes: B01D 39/18 the material being cellulose or derivatives thereof cork or peat B01D39/1646 ; making filter paper D21F11/14
• CPC Class Codes: B01D 39/20 of inorganic material, e.g. asbestos paper, metallic filtering material of non-woven wires porous ceramic material C04B38/00 ; sintering metals C22C1/04; making porous sintered metal bodies B22F3/10, honeycomb filters B01D46/2418, materials used for filtering exhaust gases of an internal combustion engine F01N3/022, ceramic honeycomb structures C04B38/0006
• CPC Class Codes: B01D 61/146 comprising multiple ultrafiltration steps
• CPC Class Codes: B01D 61/16 Feed pretreatment
• CPC Class Codes: B01D 69/141 Heterogeneous membranes, e.g. containing dispersed material; Mixed matrix membranes
• CPC Class Codes: B01D 69/1411 : containing dispersed material in a continuous matrix
• CPC Class Codes: B01J 20/28014 : characterised by their form
• CPC Class Codes: B01J 20/28028 : Particles immobilised within fibres or filaments
• CPC Class Codes: C12P 21/00 : Preparation of peptides or proteins single cell protein C12N1/00

Abstract

The present invention is to a prefilter for purification systems such as affinity chromatography columns and ultrafiltration systems. The prefilter, positioned upstream of the system inlet, reduces the presence of non-specific binding (NSB) species that enter the system, thereby extending the yield, capacity and lifetime of the system. Suitable agents include but are not limited to hydrophobic entities; lipophilic entities; activated carbon; charged cation or anion entities; ligands; particles such as fumed silica, glass, controlled pore glass or derivitized version of each; silica or silicates; and combinations thereof. The material(s) can be incorporated into a variety of media such as fibers, beads, membranes and the like and then incorporated into a variety of device designs including lenticular pads, depth filters, bead containing columns, spiral wound devices, TFF devices and the like.

Claims

1. A prefilter for a protein purification system comprising one or more layers of one or more media selected to remove non-specific binding constituents from a protein containing stream wherein the prefilter is in the form of a tangential flow device with fibrous media containing one or more non-specific binding agents arranged in a series of cassettes or pods with the flow running across or tangential to the material and at least a portion of the fluid passing through the material on each pass and the device has a central distributor in fluid communication with an inlet, media attached to both side of the distributor, a collection area outside of the media that is in fluid communication with an outlet from the device.

2. A prefilter for a protein purification system comprising one or more layers of one or more media selected to remove non-specific binding constituents from a protein containing stream wherein the prefilter is in the form of a tangential flow device with fibrous media containing one or more non-specific binding agents arranged in a series of cassettes or pods with the flow running across or tangential to the material and at least a portion of the fluid passing through the material on each pass, the device has a central distributor in fluid communication with an inlet, media attached to both side of the distributor, a collection area outside of the media that is in fluid communication with an outlet from the device and wherein the media is a cellulose derived fibrous pad containing a material selected from the group consisting of glass, silicates, silica and blends thereof.

3. A prefilter for a protein purification system comprising one or more layers of one or more media selected to remove non-specific binding constituents from a protein containing stream wherein the prefilter is in the form of a tangential flow device with fibrous media containing one or more non-specific binding agents arranged in a series of cassettes or pods with the flow running across or tangential to the material and at least a portion of the fluid passing through the material on each pass, the device has a central distributor in fluid communication with an inlet, media attached to both side of the distributor, a collection area outside of the media that is in fluid communication with an outlet from the device and wherein the media is a cellulose derived fibrous pad containing a material selected from the group consisting of glass, wherein the glass is in a form selected from the group consisting of fibers, mats and controlled pore glass particles.