Mammalian cytokine complexes

• US 7,390,481 B2
• Filed: 02/11/2004
• Issued: 06/24/2008
• Est. Priority Date: 03/11/1999
• Status: Expired due to Fees

• Current Assignee: Schering Corporation (Merck & Co., Inc.)
• Original Assignee: Schering Corporation (Merck & Co., Inc.)
• Inventors: Bazan, J. Fernando, Kastelein, Robert A., Oppmann, Birgit, Timans, Jacqueline C.
• Primary Examiner(s): HAYES, ROBERT CLINTON
• Application Number:
  US10/778,002
• Publication Number:
  US 20040192891A1
• Time in Patent Office: 1,595 Days
• Field of Search: None
• US Class Current: 424/85.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/54 Interleukins [IL]

Abstract

Purified genes encoding cytokine from a mammal, reagents related thereto including purified proteins, specific antibodies, and nucleic acids encoding this molecule are provided. Methods of using said reagents and diagnostic kits are also provided.

Claims

1. An isolated soluble cytokine complex comprising the mature forms of SEQ ID NO:

2 and SEQ ID NO;

12.

2. The cytokine complex of claim 1, wherein the cytokine complex binds to a receptor complex comprising human CTNF-R, human gp130, and human LIF-R.

3. The cytokine complex of claim 1, wherein the cytokine complex is attached to at least one detection tag.

4. The cytokine complex of claim 3, wherein the detection tag is selected from the group consisting of FLAG, His6, and Ig domain.

5. A kit comprising the cytokine complex of claim 1, wherein the kit contains:

a) a compartment comprising the cytokine complex; and

b) instructions for use or disposal of reagents in the kit.

6. The cytokine complex of claim 1, wherein the cytokine complex induces neuromodulators selected from the group consisting of cholecystokinin (CCK), vasoactive intestinal polypeptide (VIP), substance P (SP), and somatostatin (SOM).

7. A composition comprising the cytokine complex of claim 1 and an aqueous carrier.

8. An isolated soluble cytokine fusion protein comprising a mature form of SEQ ID NO:

2 fused to a mature form of SEQ ID NO;

12.

9. The cytokine fusion protein of claim 8, wherein the cytokine complex binds to a receptor complex comprising human CTNF-R, human gp130, and human LIF-R.

10. The cytokine fusion protein of claim 8, wherein the cytokine complex is attached to at least one detection tag.

11. The cytokine fusion protein of claim 10, wherein the detection tag is selected from the group consisting of FLAG, His6, and Ig domain.

12. A kit comprising the cytokine fusion protein of claim 8, wherein the kit contains:

a) a compartment comprising the cytokine fusion protein; and

b) instructions for use or disposal of reagents in the kit.

13. A composition comprising the cytokine fusion protein of claim 8 and an aqueous carrier.

14. The cytokine complex of claim 8, wherein the cytokine fusion protein induces neuromodulators selected from the group consisting of cholecystokinin (CCK), vasoactive intestinal polypeptide (VIP), substance P (SP), and somatostatin (SOM).