Self-contained adipose derived stem cell processing unit

• US 7,390,484 B2
• Filed: 07/07/2003
• Issued: 06/24/2008
• Est. Priority Date: 12/07/2001
• Status: Active Grant

• Current Assignee: Lorem Vascular Pte. Ltd.
• Original Assignee: Cytori Therapeutics Incorporated
• Inventors: Fraser, John K., Hedrick, Marc H.
• Primary Examiner(s): LANKFORD JR, LEON B
• Application Number:
  US10/614,644
• Publication Number:
  US 20040106196A1
• Time in Patent Office: 1,814 Days
• Field of Search: 424/93.7, 424/325, 424/366, 606/1, 606/131, 604/19, 604/22, 435/325, 435/366
• US Class Current: 424/93.7
• CPC Class Codes: A61B 10/0283 with vacuum aspiration, e.g. caused by retractable plunger or by connected syringe A61B10/025, A61B10/0266 take precedence
• CPC Class Codes: A61K 35/28 Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
• CPC Class Codes: A61K 35/35 Fat tissue; Adipocytes; Stromal cells; Connective tissues adipose-derived stem cells A61K35/28; collagen A61K38/39
• CPC Class Codes: A61K 9/0019 Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner non-active ingredients are additionally classified in A61K47/00
• CPC Class Codes: A61M 1/76 Handpieces specially for suction-irrigation A61M1/774, aspiration tips A61M1/84
• CPC Class Codes: A61M 2202/08 Lipoids
• CPC Class Codes: A61P 1/00 Drugs for disorders of the alimentary tract or the digestive system
• CPC Class Codes: A61P 1/04 for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
• CPC Class Codes: A61P 1/16 for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
• CPC Class Codes: A61P 11/00 Drugs for disorders of the respiratory system
• CPC Class Codes: A61P 13/12 of the kidneys
• CPC Class Codes: A61P 17/00 Drugs for dermatological disorders
• CPC Class Codes: A61P 17/02 for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders
• CPC Class Codes: A61P 19/02 for joint disorders, e.g. arthritis, arthrosis
• CPC Class Codes: A61P 19/04 for non-specific disorders of the connective tissue
• CPC Class Codes: A61P 19/08 for bone diseases, e.g. rachitism, Paget's disease
• CPC Class Codes: A61P 19/10 for osteoporosis
• CPC Class Codes: A61P 21/00 Drugs for disorders of the muscular or neuromuscular system
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 27/02 : Ophthalmic agents
• CPC Class Codes: A61P 3/00 : Drugs for disorders of the metabolism of the blood or the extracellular fluid A61P7/00
• CPC Class Codes: A61P 3/06 : Antihyperlipidemics
• CPC Class Codes: A61P 3/10 : for hyperglycaemia, e.g. antidiabetics
• CPC Class Codes: A61P 37/06 : Immunosuppressants, e.g. drugs for graft rejection
• CPC Class Codes: A61P 43/00 : Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: A61P 9/00 : Drugs for disorders of the cardiovascular system
• CPC Class Codes: A61P 9/10 : for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
• CPC Class Codes: C12M 47/04 : Cell isolation or sorting purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04
• CPC Class Codes: C12N 2506/1384 : from adipose-derived stem cells [ADSC], from adipose stromal stem cells
• CPC Class Codes: C12N 5/0667 : Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
• CPC Class Codes: C12N 5/069 : Vascular Endothelial cells

Abstract

Cells present in processed lipoaspirate tissue are used to treat patients. Methods of treating patients include processing adipose tissue to deliver a concentrated amount of stem cells obtained from the adipose tissue to a patient. The methods may be practiced in a closed system so that the stem cells are not exposed to an external environment prior to being administered to a patient. Compositions that are administered to a patient include a mixture of adipose tissue and stem cells so that the composition has a higher concentration of stem cells than when the adipose tissue was removed from the patient.

Claims

1. A self-contained adipose-derived stem cell processing unit, comprising:

a tissue collection container that is configured to receive unprocessed adipose tissue that is removed from a patient, wherein said tissue collection chamber is defined by a closed system;

a first filter that is disposed within said tissue collection container, wherein said first filter is configured to retain a first component of said unprocessed adipose tissue and pass a second component of said unprocessed adipose tissue, such that said first filter separates said first component from said second component, and wherein said first component comprises a cell population that comprises adipose-derived stem cells and said second component comprises lipid, blood, mature adipocytes, and saline;

a cell collection chamber, which is configured to receive said first component comprising a cell population that comprises adipose-derived stem cells from said tissue collection container, wherein said cell collection container is within said closed system;

a conduit configured to allow passage of said first component comprising a cell population comprising adipose-derived stem cells from said tissue collection chamber to said cell collection container while maintaining a closed system;

a cell concentrator disposed within said cell collection chamber, which is configured to facilitate the concentration of said first component comprising a cell population that comprises adipose-derived stem cells so as to obtain a concentrated population of cells that comprise adipose-derived stem cells, wherein said cell concentrator comprises a centrifuge or a spinning membrane filter; and

an outlet configured to allow the aseptic removal of said concentrated population of cells that comprise adipose-derived stem cells.

2. The cell processing unit of claim 1, wherein said tissue collection container is configured to receive lipoaspirate.

3. The cell processing unit of claim 2, wherein said tissue collection container is coupled to a cannula.

4. The cell processing unit of claim 2, wherein said tissue collection container is coupled to a suction device.

5. The cell processing unit of claim 1, wherein said tissue collection container is configured to receive adipose tissue that is removed by excisional lipectomy.

6. The cell processing unit of claim 1, wherein said first filter has a pore size of about 20 μ

m to about 5 mm.

7. The cell processing unit of claim 6, wherein said first filter is a mesh with a pore size of about 265 μ

m.

8. The cell processing unit of claim 1, wherein said tissue collection container is configured to receive an additive.

9. The cell processing unit of claim 8, wherein said tissue collection container is configured to agitate said adipose tissue and said additive.

10. The cell processing unit of claim 1, wherein said adipose-derived stem cell processing unit comprises a centrifuge.

11. The cell processing unit of claim 1, wherein said cell collection container comprises a spinning membrane filter.

12. The cell processing unit of claim 1, further comprising a second filter configured to pass said population of cells that comprises adipose stem cells and retain material larger than said population of cells.

13. The cell processing unit of claim 12, wherein said second filter is within said cell collection container.

14. The cell processing unit of claim 1, wherein said cell collection container is configured to receive an additive.

15. The cell processing unit of claim 1, wherein said cell collection container is configured to wash said concentrated population of cells that comprise adipose-derived stem cells.

16. The cell processing unit of claim 1, wherein the cell collection container is configured to separate said concentrated population of cells that comprise adipose-derived stem cells based on a difference in electrical charge.

17. The cell processing unit of claim 1, wherein the cell collection container further comprises a means to agitate said concentrated population of cells that comprise adipose-derived stem cells.

18. The cell processing unit of claim 14, wherein the cell collection container further comprises a means to agitate said concentrated population of cells that comprise adipose-derived stem cells.

19. The cell processing unit of claim 1, wherein the cell collection container further comprises a means for washing said concentrated population of cells that comprise adipose-derived stem cells.

20. The cell processing unit of claim 14, wherein the cell collection container further comprises a means for washing said concentrated population of cells that comprise adipose-derived stem cells.

21. The cell processing unit of claim 1, wherein said cell collection container comprises a means to separate said population of cells that comprise adipose-derived stem cells by density gradient.

22. The cell processing unit of claim 1, wherein said cell collection container comprises a means to separate said population of cells that comprise adipose-derived stem cells by adherence to plastic.

23. The cell processing unit of claim 1, wherein said cell collection container comprises a means to culture cells.

24. The cell processing unit of claim 1, further comprising a mixing container, wherein said mixing container is joined to said tissue collection container via a conduit configured to maintain a closed system, which wherein said mixing container comprises a port for the addition of an additive, wherein said mixing container is within said closed system, and wherein said cell collection container is positioned between said tissue collection container and said mixing chamber.

25. The cell processing unit of claim 24, wherein said second filter is positioned between said cell collection container and said mixing container.

26. The cell processing unit of claim 25, wherein said second filter has a pore size of between 20 μ

m and 200 μ

m.

27. The cell processing unit of claim 1, further comprising a temperature controller.