Methods and materials for nanocrystalline surface coatings and attachment of peptide amphiphile nanofibers thereon

• US 7,390,526 B2
• Filed: 02/11/2004
• Issued: 06/24/2008
• Est. Priority Date: 02/11/2003
• Status: Expired due to Term

• Current Assignee: Northwestern University
• Original Assignee: Northwestern University
• Inventors: Spoerke, Erik D., Stupp, Samuel I., Anthony, Shawn G., Niece, Krista L.
• Primary Examiner(s): Lukton; David
• Application Number:
  US10/777,030
• Publication Number:
  US 20040258726A1
• Time in Patent Office: 1,595 Days
• Field of Search: 427/2.26, 427/2.27, 514/2, 514 17- 18
• US Class Current: 427/2.27
• CPC Class Codes: A61L 27/32 Phosphorus-containing materials, e.g. apatite
• CPC Class Codes: A61L 27/425 of phosphorus containing material, e.g. apatite
• CPC Class Codes: A61L 31/086 Phosphorus-containing materials, e.g. apatite
• CPC Class Codes: A61L 31/123 of phosphorus-containing materials, e.g. apatite
• CPC Class Codes: C12N 2533/18 Calcium salts, e.g. apatite, Mineral components from bones, teeth, shells
• CPC Class Codes: C12N 2533/50 Proteins
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06

Abstract

Biocompatible composites comprising peptide amphiphiles and surface modified substrates and related methods for attachment thereon.

Claims

1. A nanotextured biocompatible composite, comprising a biocompatible substrate and a mineral phase on said substrate comprising a calcium phosphate component, nanofibers of peptide amphiphiles, and poly(L-lysine), wherein said nanofibers are coupled to the poly(L-lysine) on said calcium phosphate component.

2. The composite of claim 1 wherein the calcium content of said mineral phase is less than stoichiometric, and said poly(L-lysine) is incorporated within said calcium phosphate.

3. The composite of claim 1 wherein said mineral phase is reactive with at least one of an acid and degradative enzyme.

4. The composite of claim 1 wherein at least one of said peptide amphiphiles comprises a carboxy functionality.

5. The composite of claim 4 wherein at least one of said peptide amphiphiles comprises the sequence, RGD.

6. The composite of claim 4 further comprising a mammalian preosteoblast cell culture.

7. The composite of claim 1 wherein said substrate comprises titanium.