Cellular fibronectin as a diagnostic marker in stroke and methods of use thereof
First Claim
1. A method of determining presence or risk of cerebral injury in a human subject, the method comprising:
- obtaining a test sample from a human subject;
analyzing the obtained test sample for presence or amount or both presence and amount of (1) cellular fibronectin and (2) one or more additional markers both proteomic and non-proteomic for, or mass spectrometry peak levels from, any of categories of apoptosis, cellular adhesion, cellular injury, coagulation, glial activation, inflammatory mediation, myelin breakdown, thrombosis, and vascular damage; and
thencorrelating (1) the presence or amount of said cellular fibronectin and said one or more additional markers or peak levels, with (2) clinical patient information, other than the cellular fibronectin and said one or more makers or peak levels for cerebral injury, in order to deduce a probability of present or future risk or present and future risk of a cerebral injury for the subject; and
thenproviding the deduced probability to a clinician who treats the cerebral injury in the human subject in accordance with the deduced probability.
0 Assignments
0 Petitions
Accused Products
Abstract
Methods for the diagnosis and evaluation of stroke and stroke sub-type employ a variety of bio-markers including cellular fibronectin (c-Fn) assembled as a panel for stoke diagnosis and evaluation. Methods are disclosed for selecting markers and correlating their combined levels with a clinical outcome of interest. In various aspects the methods permit early detection and differentiation of stroke subtypes, determination of the prognosis of a patient presenting stroke symptoms, and identification of a patient at risk for early hematoma growth and/or malignant massive cerebral artery infarction. The disclosed methods provide rapid, sensitive and specific assays to greatly increase the number of patients that can receive beneficial stroke treatment and therapy, and to reduce the human and economic costs associated with incorrect stroke diagnosis.
-
Citations
19 Claims
-
1. A method of determining presence or risk of cerebral injury in a human subject, the method comprising:
-
obtaining a test sample from a human subject; analyzing the obtained test sample for presence or amount or both presence and amount of (1) cellular fibronectin and (2) one or more additional markers both proteomic and non-proteomic for, or mass spectrometry peak levels from, any of categories of apoptosis, cellular adhesion, cellular injury, coagulation, glial activation, inflammatory mediation, myelin breakdown, thrombosis, and vascular damage; and
thencorrelating (1) the presence or amount of said cellular fibronectin and said one or more additional markers or peak levels, with (2) clinical patient information, other than the cellular fibronectin and said one or more makers or peak levels for cerebral injury, in order to deduce a probability of present or future risk or present and future risk of a cerebral injury for the subject; and
thenproviding the deduced probability to a clinician who treats the cerebral injury in the human subject in accordance with the deduced probability. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
-
Specification