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Methods and compositions for interaction trap assays

  • US 7,393,318 B2
  • Filed: 03/16/2004
  • Issued: 07/01/2008
  • Est. Priority Date: 05/16/2000
  • Status: Expired due to Term
First Claim
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1. A population of host cells, each host cell comprising:

  • (a) a polynucleotide encoding a fusion protein, the fusion protein comprising(i) an activation tag; and

    (ii) a polypeptide sequence to be assayed for its interaction with a DNA sequence,(b) a transcriptional regulatory sequence comprising one or more binding sites for a zinc finger DNA-binding domain;

    (c) a reporter gene operably linked to the transcriptional regulatory sequence, wherein expression of the reporter gene is modulated when the polypeptide sequence interacts with one or more of the binding sites;

    wherein at least 107 unique pairs of the one or more binding sites and fusion proteins that bind to the 107 unique pairs of the one or more binding sites are represented in the population of host cells.

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