3-Hydroxypropionic acid and other organic compounds

• US 7,393,676 B2
• Filed: 10/09/2006
• Issued: 07/01/2008
• Est. Priority Date: 11/20/2000
• Status: Expired due to Fees

• Current Assignee: Cargill Incorporated
• Original Assignee: Cargill Incorporated
• Inventors: Gokarn, Ravi R., Gort, Steven John, Selifonova, Olga V., Selmer, Thorsten, Jessen, Holly Jean, Buckel, Wolfgang
• Primary Examiner(s): Saidha; Tekchand
• Application Number:
  US11/539,856
• Publication Number:
  US 20070184524A1
• Time in Patent Office: 631 Days
• Field of Search: 435/232, 536/23.2
• US Class Current: 435/232
• CPC Class Codes: C12N 15/52 Genes encoding for enzymes or proenzymes
• CPC Class Codes: C12N 9/0004 Oxidoreductases (1.)
• CPC Class Codes: C12P 7/18 polyhydric
• CPC Class Codes: C12P 7/40 containing a carboxyl group including Peroxycarboxylic acids
• CPC Class Codes: C12P 7/42 Hydroxy-carboxylic acids
• CPC Class Codes: C12P 7/52 Propionic acid; Butyric acids
• CPC Class Codes: C12P 7/625 Polyesters of hydroxy carboxylic acids
• CPC Class Codes: Y02P 20/52 using catalysts, e.g. selective catalysts

Abstract

Methods and materials related to producing 3-HP as well as other organic compounds are disclosed. Specifically, isolated nucleic acids, polypeptides, host cells, and methods and materials for producing 3-HP and other organic compounds are disclosed.

Claims

1. An isolated polypeptide comprising an amino acid sequence selected from the group consisting of:

(a) the sequence set forth in SEQ ID NO;

41;

(b) a portion of SEQ ID NO;

41 having 23 or more contiguous amino acid residues of SEQ ID NO;

41, wherein the portion of SEQ ID NO;

41 can function as a 3-hydroxypropionyl-CoA dehydratase;

(c) the sequence set forth in SEQ ID NO;

41 containing 5 conservative substitutions; and

(d) the sequence set forth in SEQ ID NO;

41 containing 10 conservative substitutions.

2. An isolated polypeptide comprising the amino acid sequence set forth in SEQ ID NO:

41.

3. An isolated polypeptide consisting of the amino acid sequence set forth in SEQ ID NO:

41.

4. An isolated polypeptide comprising the amino acid sequence set forth in SEQ ID NO:

41 containing 5 conservative substitutions.

5. An isolated polypeptide comprising the amino acid sequence set forth in SEQ ID NO:

41 containing 10 conservative substitutions.

6. An isolated polypeptide comprising at least 95% sequence identity to SEQ ID NO:

41, wherein the polypeptide can function as a 3-hydroxypropionyl-CoA dehydratase.

7. The isolated polypeptide of claim 6, wherein the polypeptide comprises at least 97% sequence identity to SEQ ID NO:

41, wherein the polypeptide can function as a 3-hydroxypropionyl-CoA dehydratase.

8. The isolated polypeptide of claim 6, wherein the polypeptide comprises at least 98% sequence identity to SEQ ID NO:

41, wherein the polypeptide can function as a 3-hydroxypropionyl-CoA dehydratase.

9. The isolated polypeptide of claim 6, wherein the polypeptide comprises at least 99% sequence identity to SEQ ID NO:

41, wherein the polypeptide can function as a 3-hydroxypropionyl-CoA dehydratase.

10. An isolated polypeptide comprising a portion of SEQ ID NO:

41 having 23 or more contiguous amino acid residues of-SEQ ID NO;

41, wherein the portion of SEQ ID NO;

41 can function as a 3-hydroxypropionyl-CoA dehydratase.

11. The isolated polypeptide of claim 10, wherein the portion of SEQ ID NO:

41 has 25 or more contiguous amino acid residues of-SEQ ID NO;

41, wherein the portion of SEQ ID NO;

41 can function as a 3-hydroxypropionyl-CoA dehydratase.

12. The isolated polypeptide of claim 10, wherein the portion of SEQ ID NO:

41 has 30 or more contiguous amino acid residues of-SEQ ID NO;

41, wherein the portion of SEQ ID NO;

41 can function as a 3-hydroxypropionyl-CoA dehydratase.