Peptides for enhanced cell attachment and growth

• US 7,399,630 B2
• Filed: 11/27/2006
• Issued: 07/15/2008
• Est. Priority Date: 08/15/2003
• Status: Expired due to Term

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Liebmann-Vinson, Andrea, Heidaran, Mohammad A., Chaney, Bryce Nelson, Guarino, Richard David
• Primary Examiner(s): Monshipouri; Maryam
• Assistant Examiner(s): Tsay; Marsha M
• Application Number:
  US11/604,530
• Publication Number:
  US 20070077651A1
• Time in Patent Office: 596 Days
• Field of Search: 435/325, 435/240, 435/243, 435/289.1, 530/330, 530/402
• US Class Current: 435/289.1
• CPC Class Codes: B33Y 80/00 Products made by additive manufacturing
• CPC Class Codes: C07K 5/0806 the side chain containing 0 or 1 carbon atoms, i.e. Gly, Ala
• CPC Class Codes: C07K 5/1008 the side chain containing 0 or 1 carbon atoms, i.e. Gly, Ala
• CPC Class Codes: C07K 5/1019 with the first amino acid being basic
• CPC Class Codes: C07K 7/06 having 5 to 11 amino acids

Abstract

The present invention relates to cell adhesion promoting (“CAP”) peptide combinations that promote cell attachment or cell adhesion to culture surfaces that are otherwise cell adhesion resistant “CAR”. The invention provides combination of peptides that, when covalently coupled to a CAR layer such as hyaluronic acid that has been created on a polystyrene surface, promote cell attachment, growth differentiation, and execution of other desired cellular functions in culture.

Claims

1. A cell culture vessel for culturing adherent cells, said vessel comprising a polymer surface, a hyaluronic acid (HA) layer bonded to said polymer surface, and one or more peptides covalently bound to the HA layer, wherein said peptides promote adherence of cells that do not adhere to the HA layer in the absence of said peptides, and wherein each of said one or more peptides comprises:

(a) one or more peptide sequences selected from the group consisting of SEQ ID NO;

2 (EFIKY), SEQ ID NO;

3 (EEEKV), SEQ ID NO;

4 (GHK), SEQ ID NO;

6 (KKLVY), SEQ ID NO;

8 (AFAFK), SEQ ID NO;

10 (KMLIY), SEQ ID NO;

11 (KSYYY), SEQ ID NO;

12 (AKKMV), SEQ ID NO;

14 (AFIPV), SEQ ID NO;

15 (KKMYY), and SEQ ID NO;

16 (AIKKK);

(b) a chemically or recombinantly prepared peptide multimer that includes at least two repeats of any one or a combination of SEQ ID NOs;

2-4, 6, 8, 10-12, and 14-16;

or

(c) a cell adhesion promoting variant of any one or more of SEQ ID NOs;

2-4, 6, 8, 10-12, and 14-16, said variant having a conservative substitution of a single amino acid residue, wherein said variant has the ability to promote cell attachment or growth.

2. The cell culture vessel of claim 1, wherein said cells are fibroblasts or osteoblasts.

3. The cell culture vessel of claim 1, wherein said cells are liver cells.

4. The cell culture vessel of claim 1, wherein said cells are human or rat primary hepatocytes or porcine hepatocytes.

5. The cell culture vessel of claim 1, wherein said cell culture vessel is a multiwell plate.

6. The cell culture vessel of claim 1, wherein said polymer surface is formed from a flexible material.

7. The system cell culture vessel of claim 1, wherein said polymer surface is selected from the group consisting of polystyrene, polypropylene, polyethylene tereplithalate, polylactide, cellulose, and combinations thereof.

8. The cell culture vessel of claim 7, wherein said polymer surface is polystyrene.

9. A cell culture vessel for culturing adherent liver cells, said vessel comprising a polymer surface, a cell adhesion resistant (CAR) material bonded to said polymer surface, and one or more peptides covalently bound to the CAR material, wherein said peptides promote adherence of liver cells that do not adhere to the CAR material in the absence of said peptides, and wherein each of said one or more peptides comprises:

(a) one or more peptide sequences selected from the group consisting of SEQ ID NO;

2 (EFIKY), SEQ ID NO;

3 (EEEKV), SEQ ID NO;

4 (GHK), SEQ ID NO;

6 (KKLVY), SEQ ID NO;

8 (AFAFK), SEQ ID NO;

10 (KMLIY), SEQ ID NO;

11 (KSYYY), SEQ ID NO;

12 (AKKMV), SEQ ID NO;

14 (AFIPV), SEQ ID NO;

15 (KKMYY), and SEQ ID NO;

16 (AIKKK);

(b) a chemically or recombinantly prepared peptide multimer that includes at least two repeats of any one or a combination of SEQ ID NOs;

2-4, 6, 8, 10-12, and 14-16;

or

(c) a cell adhesion promoting variant of any one or more of SEQ ID NOs;

2-4, 6, 8, 10-12, and 14-16, said variant having a conservative substitution of a single amino acid residue, wherein said variant has the ability to promote liver cell attachment or growth.

10. The cell culture vessel of claim 9, wherein said cell adhesion resistant (CAR) material is selected from the group consisting of hyaluronic acid (HA), alginic acid (AA), polyhydroxyethyl methacrylate (Poly-HEMA), polyethylene glycol (PEG), glyme, polyisopropylacrylamide, and combinations thereof.

11. The cell culture vessel of claim 10, wherein said cell adhesion resistant (CAR) material is hyaluronic acid (HA).

12. The cell culture vessel of claim 9 wherein said liver cells are human or rat primary hepatocytes or porcine hepatocytes.

13. The cell culture vessel of claim 9, wherein said cell culture vessel is a multiwell plate.

14. The cell culture vessel of claim 9, wherein said polymer surface is formed from a flexible material.

15. The cell culture vessel of claim 9, wherein said polymer surface is selected from the group consisting of polystyrene, polypropylene, polyethylene terephthalate, polylactide, cellulose, and combinations thereof.

16. The cell culture vessel of claim 15, wherein said polymer surface is polystyrene.