High affinity nucleic acid ligands to lectins
First Claim
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1. A method of inhibiting binding between L-selectin and a carbohydrate ligand of said L-selectin, comprising the step of contacting a nucleic acid ligand to said L-selectin with said L-selectin wherein said nucleic acid ligand to L-selectin is selected from the group consisting of SEQ ID NO:
- 67-117, 129-196, and 293-388.
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Abstract
This invention discloses high-affinity oligonucleotide ligands to lectins, specifically nucleic acid ligands having the ability to bind to the lectins, wheat germ agglutinin, L-selectin, E-selectin and P-selectin. Also disclosed are the methods for obtaining such ligands.
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Citations
21 Claims
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1. A method of inhibiting binding between L-selectin and a carbohydrate ligand of said L-selectin, comprising the step of contacting a nucleic acid ligand to said L-selectin with said L-selectin wherein said nucleic acid ligand to L-selectin is selected from the group consisting of SEQ ID NO:
- 67-117, 129-196, and 293-388.
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2. A method of inhibiting binding between P-selectin and a carbohydrate ligand of said P-selectin, comprising the step of contacting a nucleic acid ligand to said P-selectin with said P-selectin wherein said nucleic acid ligand to P-selectin is selected from the group consisting of SEQ ID NO:
- 199-247, and 251-290.
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3. A method of modulating leukocyte adhesion or migration, comprising inhibiting binding between a P- or L-selectin on a first cell and a ligand to said selectin on a second cell, said inhibiting comprising the step of permitting a nucleic acid ligand to the selectin to interact with the first cell, wherein the first cell is a leukocyte selected from the group consisting of neutrophils and monocytes, the second cell is a vascular endothelial cell and wherein said nucleic acid ligand is selected from the group consisting of SEQ ID NO:
- 67-117, 129-196, 199-247, 251-290 and 293-388.
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4. A method for modulating platelet adhesion, comprising inhibiting binding between a P-selectin on a first cell and a ligand to said P-selectin on a second cell, said inhibiting comprising the step of permitting a nucleic acid ligand to said P-selectin to interact with the first cell, wherein the first cell is a platelet, wherein said nucleic acid ligand to said P-selectin is selected from the group consisting of SEQ ID NO:
- 199-247 and 251-290.
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5. A method of modulating leukocyte adhesion or migration, comprising inhibiting binding between an L-selectin on a first cell and a ligand to said L-selectin on a second cell, said inhibiting comprising the step of permitting a nucleic acid ligand to said L-selectin to interact with the first cell, wherein the first cell is a leukocyte selected from the group consisting of lymphocytes, granulocytes, neutrophils and monocytes, the second cell is a vascular endothelial cell or a lymphatic cell and wherein said nucleic acid ligand to said L-selectin is selected from the group consisting of SEQ ID NO:
- 67-117, 129-196 and 293-388.
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6. A method of modulating lymphocyte adhesion, migration or activation comprising inhibiting binding between an L-selectin on a first cell and a ligand to said L-selectin on a second cell, said inhibiting comprising the step of permitting a nucleic acid ligand to said L-selectin to interact with the first cell, wherein the first cell is a lymphocyte, the second cell is a vascular endothelial cell and wherein said nucleic acid ligand is selected from the group consisting of SEQ ID NO:
- 67-117, 129-196 and 293-388.
- 7. A method of inhibiting binding between P-selectin and a carbohydrate ligand of said P-selectin, comprising the step of contacting a nucleic acid ligand to said P-selectin with said P-selectin wherein said nucleic acid ligand to P-selectin comprises SEQ ID NO:
- 12. A method of modulating leukocyte adhesion or migration, comprising inhibiting binding between a P-selectin on a first cell and a ligand to said P-selectin on a second cell, said inhibiting comprising the step of permitting a nucleic acid ligand to the selectin to interact with the first cell, wherein the first cell is a leukocyte selected from the group consisting of neutrophils and monocytes, the second cell is a vascular endothelial cell and wherein said nucleic acid ligand comprises SEQ ID NO:
- 17. A method for modulating platelet adhesion, comprising inhibiting binding between a P-selectin on a first cell and a ligand to said P-selectin on a second cell, said inhibiting comprising the step of permitting a nucleic acid ligand to said P-selectin to interact with the first cell, wherein the first cell is a platelet, wherein said nucleic acid ligand to said P-selectin comprises SEQ ID NO:
Specification