Device with integrated microfluidic and electronic components

• US 7,402,279 B2
• Filed: 10/31/2002
• Issued: 07/22/2008
• Est. Priority Date: 10/31/2002
• Status: Active Grant

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Schembri, Carol T
• Primary Examiner(s): Warden; Jill
• Assistant Examiner(s): LEVKOVICH, NATALIA A
• Application Number:
  US10/286,319
• Publication Number:
  US 20040086424A1
• Time in Patent Office: 2,091 Days
• Field of Search: 422/58, 422/102, 436/180, 436/6, 435/6
• US Class Current: 422/504
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/143 Quality control, feedback systems
• CPC Class Codes: B01L 2300/024 Storing results with means integrated into the container
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0812 Bands; Tapes
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0825 Test strips
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2300/123 Flexible; Elastomeric
• CPC Class Codes: B01L 2300/168 Specific optical properties, e.g. reflective coatings
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/505 flexible containers not provided for above
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: C12Q 2565/629 being a microfluidic device
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

Devices having microfluidic features and arrays joined to electronics components are described. In an embodiment, the array includes a flexible array substrate. The electronics components have circuitry that may e.g. detect reactions or control conditions on the device via a feedback loop. Modular architecture provides for different combinations of microfluidic components, array components, and/or electronics components that can be used to create customized processing and analysis tools.

Claims

1. A device comprising:

a) a microfluidic component having a microfluidic feature for carrying a fluid of interest;

b) an array component comprising a flexible array substrate supporting an addressable collection of probes, said array component bonded to and in operable association with said microfluidic feature of the microfluidic component for analyzing said fluid of interest, wherein the flexible array substrate includes;

a flexible support,

a flexible base mounted on said flexible support,

a reflective layer mounted on said base, the reflective layer comprising multiple dielectric layers, said dielectric layers being constructed by repeating pairs of transparent dielectrics, each dielectric in a pair having a different index of refraction from the other dielectric in the pair, and

a transparent layer mounted on said reflective layer; and

c) an electronics component joined to said microfluidic component, wherein said electronics component is formed of discrete electronic devices on a substrate that is bonded to said microfluidic component via at least one of the following;

contact solder, gold contact bumps, gold contact pads, and conductive adhesive, in order to provide electrical contact between the electronics component and the microfluidic component.

2. The device of claim 1 wherein the microfluidic component comprises conductive elements in electrical communication with the electronics component.

3. The device of claim 1 wherein said electronics component comprises signal detection and signal processing circuitry for evaluating a property of said fluid of interest.

4. The device of claim 3 wherein said circuitry of said electronics component has an output for providing direct feedback to said microfluidic component in response to signals from said circuitry, thereby creating a feedback loop that is isolated within said device.

5. The device of claim 1 wherein said electronics component includes a signal amplifier circuit for amplifying a signal from a signal detection subcircuit of said circuitry.

6. The device of claim 1 wherein the electronic component is adapted to detect a signal resulting from a probe being bound to a corresponding target in the fluid of interest.

7. The device of claim 1 wherein said electronics component is an integrated circuit chip that is bonded to said microfluidic component.

8. The device of claim 1 wherein said electronics component is an integrated circuit.

9. The device of claim 1 wherein said electronics component includes firmware for signal processing and signal control.

10. The device of claim 1 further comprising a second electronics component bonded to said microfluidic component.

11. The device of claim 10 wherein said electronics component and said second electronics component are bonded on opposing sides of said microfluidic component.

12. The device of claim 1 wherein said flexible array substrate comprises a flexible base supporting, in order, the reflective layer, the transparent layer, and the addressable collection of probes.

13. The device of claim 1, wherein the reflective layer comprises a layer of a metal disposed between the flexible base and the multiple dielectric layers, wherein the metal is selected from aluminum, silver, gold, platinum, chrome, and tantalum.

14. The device of claim 13, wherein the transparent layer comprises a material selected from one of the following:

silicon dioxide, titania, and alumina.

15. The device of claim 14, wherein the metal layer is chrome.

16. The device of claim 15, wherein each of the flexible support and the flexible base comprise semi-crystalline polyetheretherketone.

17. The device of claim 14, wherein the metal layer is tantalum.

18. The device of claim 1, wherein said dielectric layers are a material selected from:

silicon dioxide, titania, and alumina.

19. The device of claim 18, wherein a first one of said optically clear dielectric layers forming one of said pairs includes a material selected from silicon dioxide and titania, wherein a second one of said transparent dielectric layers forming said one pair includes a material selected from silicon dioxide and titania, and wherein the first transparent dielectric layer and the second transparent dielectric layer are made of different materials.

20. The device of claim 19, wherein said repeating pairs of transparent dielectrics includes a number of pairs selected from:

two pairs, three pairs, and four pairs, wherein a first dielectric pair has a second dielectric pair disposed thereon, and wherein an transparent dielectric layer of the first pair is made of a different material than an transparent dielectric layer of the second pair.

21. The device of claim 19, further comprising a third dielectric pair that has a fourth dielectric pair disposed thereon, wherein the third dielectric pair is disposed on the second dielectric pair, and wherein an transparent dielectric layer of the third dielectric pair is made of a different material than an transparent dielectric layer of the fourth dielectric pair.

22. The device of claim 21, wherein the reflective layer comprises a layer of a metal disposed between the flexible base and the dielectric layer pairs, wherein the metal is selected from aluminum, silver, gold, platinum, chrome, and tantalum.

29. The integrated microfluidic device of claim 13, further comprising a transparent layer mounted on the reflective layer.

30. The integrated microfluidic device of claim 13, wherein the microfluidic feature is chosen from at least one of:

a separation channel, a detection channel, a reservoir, a valve, and a pump.

31. The integrated microfluidic device of claim 13, wherein the reflective layer further comprises a metal layer, and at least one of the dielectric layers is disposed on the metal layer.

32. The integrated microfluidic device of claim 31, wherein the metal layer is chrome.

33. The integrated microfluidic device of claim 13, wherein each of the flexible support and the flexible base comprise semi-crystalline polyetheretherketone.

34. The integrated microfluidic device of claim 31, wherein the metal layer is tantalum.

35. The integrated microfluidic device of claim 31, wherein each of the dielectrics is a material selected from:

silicon dioxide, titania, and alumina, and wherein the metal layer is a metal selected from aluminum, silver, gold, platinum, chrome, and tantalum.

23. An integrated microfluidic device comprising:

a) a microfluidic component having a microfluidic feature;

b) a flexible array substrate bonded to and in fluid communication with the microfluidic feature, wherein the flexible array substrate includes;

a flexible support,

a flexible base mounted on said flexible support, and

a reflective layer mounted on said base, the reflective layer comprising multiple dielectric layers, said dielectric layers being constructed by repeating pairs of transparent dielectrics, each dielectric in a pair having a different index of refraction from the other dielectric in the pair; and

c) an electronics component joined to said microfluidic component, said electronics component having circuitry to control a process within said microfluidic component, and wherein said electronics component is formed of discrete electronic devices on a substrate that is bonded to said microfluidic component.

24. The integrated microfluidic device of claim 23 wherein said circuitry of said electronics component enables control of at least one of a reaction, concentration, or separation process that occurs within said microfluidic features of said microfluidic component.

25. The integrated microfluidic device of claim 23 wherein said circuitry of said electronics component enables control of the flow of fluids within said microfluidic component.

26. The integrated microfluidic device of claim 23 wherein said circuitry of said electronics component enables at least one of voltage control, current control, electrical field control, and magnetic field control.

27. The integrated microfluidic device of claim 23 wherein said circuitry of said electronics component further enables signal processing of signals generated by said electronics component in cooperation with said microfluidic component.

28. The integrated microfluidic device of claim 23 wherein the microfluidic component and the electronics component each are of modular construction.