Method of immobilizing molecules onto a solid phase substrate and method of fabricating a biosensor using the method
First Claim
1. A method of immobilization, comprising:
- immobilizing a plurality of first molecules and a plurality of second molecules to a solid phase substrate by putting a solution of the plurality of first and second molecules in contact with the solid phase substrate,each of the plurality of first molecules having a first functional portion that is used for recognizing a first object to be examined,each of the plurality of first molecules including a first joint portion, the first functional portion being immobilized through the first joint portion that is capable of binding to the solid phase substrate,each of the plurality of second molecules having a second functional portion that is different from the first functional portion and that functions as a mediator of the first functional portion, andeach of the plurality of second molecules including a second joint portion, the second functional portion being immobilized through the second joint portion that is capable of binding to the solid phase substrate.
1 Assignment
0 Petitions
Accused Products
Abstract
Aspects of the invention can provide a method capable of easily realizing immobilization with the optimum density derived from a concentration control and without phase separation in coadsorption of a number of molecules. The immobilization method can include the step of dissolving a plurality of molecules to be immobilized to a solid phase substrate with a solvent to obtain a solution of the plurality of molecules, and the step of incubating the solution and the solid phase substrate in touch therewith. Each of the molecules can include a solid phase substrate joint portion having a jointing property to the solid phase substrate, a functional portion having a specific function, and a linker portion positioned between the solid phase substrate joint portion and the functional portion.
11 Citations
23 Claims
-
1. A method of immobilization, comprising:
-
immobilizing a plurality of first molecules and a plurality of second molecules to a solid phase substrate by putting a solution of the plurality of first and second molecules in contact with the solid phase substrate, each of the plurality of first molecules having a first functional portion that is used for recognizing a first object to be examined, each of the plurality of first molecules including a first joint portion, the first functional portion being immobilized through the first joint portion that is capable of binding to the solid phase substrate, each of the plurality of second molecules having a second functional portion that is different from the first functional portion and that functions as a mediator of the first functional portion, and each of the plurality of second molecules including a second joint portion, the second functional portion being immobilized through the second joint portion that is capable of binding to the solid phase substrate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
-
-
14. A biosensor, comprising:
-
a substrate; a plurality of first molecules that are immobilized to the substrate, each of the plurality of first molecules having a first functional portion that is used for recognizing a first object to be examined, and each of the plurality of first molecules including a first joint portion, the first functional portion being immobilized through the first joint portion that is capable of binding to the substrate; and a plurality of second molecules that are immobilized to the substrate, each of the plurality of second molecules having a second functional portion that is different from the first functional portion and that functions as a mediator of the first functional portion, and each of the plurality of second molecules including a second joint portion, the second functional portion being immobilized through the second joint portion that is capable of binding to the substrate. - View Dependent Claims (15, 16, 17, 18, 19, 20, 21, 22)
-
-
23. A method of immobilization, comprising:
-
immobilizing a plurality of first molecules and a plurality of second molecules to a solid phase substrate by putting a solution of the plurality of first and second molecules in contact with the solid phase substrate, each of the plurality of first molecules having a first functional portion that is used for recognizing a first object to be examined, each of the plurality of first molecules including a first joint portion, the first functional portion being immobilized through the first joint portion that is capable of binding to the solid phase substrate, each of the plurality of second molecules having a second functional portion that is different from the first functional portion, each of the plurality of second molecules including a second joint portion, the second functional portion being immobilized through the second joint portion that is capable of binding to the solid phase substrate, and the plurality of first molecules and the plurality of second molecules constituting a self-assembled monomolecular film.
-
Specification