Desaturases and methods of using them for synthesis of polyunsaturated fatty acids
First Claim
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1. A transgenic organism, comprising a cell comprising a first recombinant nucleic acid molecule comprising a promoter sequence operably linked to a first heterologous nucleic acid molecule that:
- (a) hybridizes under high-stringency conditions with a nucleic acid probe, the probe comprising a sequence as shown in SEQ ID NO;
3; and
(b) encodes a protein having Δ
8 fatty acid desaturase activity, wherein the transgenic organism is a plant or a yeast.
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Abstract
The amino acid and nucleic acid sequences of a Δ5-desaturase enzyme and a Δ8-desaturase enzyme are disclosed. The nucleic acid sequences can be used to design recombinant DNA constructs and vectors. These vectors can then be used to transform various organisms, including for example, plants and yeast. The transformed organisms will then produce polyunsaturated fatty acids. The amino acid sequences are useful for generating enzyme-specific antibodies that are useful for identifying the desaturases.
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Citations
20 Claims
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1. A transgenic organism, comprising a cell comprising a first recombinant nucleic acid molecule comprising a promoter sequence operably linked to a first heterologous nucleic acid molecule that:
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(a) hybridizes under high-stringency conditions with a nucleic acid probe, the probe comprising a sequence as shown in SEQ ID NO;
3; and(b) encodes a protein having Δ
8 fatty acid desaturase activity, wherein the transgenic organism is a plant or a yeast. - View Dependent Claims (3, 4, 5, 6, 7, 8)
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2. A transgenic plant, comprising a cell comprising a first recombinant nucleic acid molecule comprising a promoter sequence operably linked to a first heterologous nucleic acid molecule that:
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(a) hybridizes under high-stringency conditions with a nucleic acid probe, the probe comprising a sequence as shown in SEQ ID NO;
3; and(b) encodes a protein having Δ
8 fatty acid desaturase activity. - View Dependent Claims (18, 19)
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9. A transgenic organism, comprising a cell comprising a first recombinant nucleic acid molecule that comprises a promoter sequence operably linked to a first heterologous nucleic acid molecule that:
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(a) has at least 95% sequence identity with a nucleic acid sequence as shown in SEQ ID NO;
3; and(b) encodes a protein having Δ
8 fatty acid desaturase activity, wherein the transgenic organism is a plant or a yeast. - View Dependent Claims (11, 12)
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10. A transgenic plant, comprising a cell comprising a first recombinant nucleic acid molecule that comprises a promoter sequence operably linked to a first heterologous nucleic acid molecule that:
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(a) has at least 95% sequence identity with a nucleic acid sequence as shown in SEQ ID NO;
3; and(b) encodes a protein having Δ
8 fatty acid desaturase activity.
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13. A transgenic organism, comprising a first recombinant nucleic acid molecule comprising a promoter sequence operably linked to a first heterologous nucleic acid molecule that encodes a protein having Δ
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8 fatty acid desaturase activity, wherein the protein comprises an amino acid sequence selected from the group consisting of;
(a) an amino acid sequence as shown in SEQ ID NO;
4;(b) an amino acid sequence that differs from that specified in (a) by one or more conservative amino acid substitutions; and (c) an amino acid sequence having at least 95% sequence identity to the sequences specified in (a) or (b), wherein the transgenic organism is a plant or a yeast. - View Dependent Claims (15, 16, 17)
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8 fatty acid desaturase activity, wherein the protein comprises an amino acid sequence selected from the group consisting of;
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14. A transgenic plant, comprising a first recombinant nucleic acid molecule comprising a promoter sequence operably linked to a first heterologous nucleic acid molecule that encodes a protein having Δ
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8 fatty acid desaturase activity, wherein the protein comprises an amino acid sequence selected from the group consisting of;
(a) an amino acid sequence as shown in SEQ ID NO;
4;(b) an amino acid sequence that differs from that specified in (a) by one or more conservative amino acid substitutions; and (c) an amino acid sequence having at least 95% sequence identity to the sequences specified in (a) or (b). - View Dependent Claims (20)
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8 fatty acid desaturase activity, wherein the protein comprises an amino acid sequence selected from the group consisting of;
Specification