Tissue ablation by shear force for sampling biological fluids and delivering active agents

• US 7,404,815 B2
• Filed: 04/30/2001
• Issued: 07/29/2008
• Est. Priority Date: 05/01/2000
• Status: Expired due to Fees

• Current Assignee: Cilag Gmbh International (Johnson & Johnson)
• Original Assignee: Lifescan Incorporated (Duv Holding Corporation)
• Inventors: Sun, Ying, Coston, Anthony F., Kollias, Nikiforos
• Primary Examiner(s): Lucchesi; Nicholas D.
• Assistant Examiner(s): Stigell; Theodore J
• Application Number:
  US09/845,956
• Publication Number:
  US 20020058902A1
• Time in Patent Office: 2,647 Days
• Field of Search: 604500-501, 604/20, 604/22, 604 46- 47, 604/890.1, 604/272, 604503-505, 604 65- 67, 600/573, 600/583, 600/309, 600/873
• US Class Current: 604/501
• CPC Class Codes: A61N 1/303 Constructional details electrodes for external use A61N1/0428

Abstract

The present invention relates to a method and a device for transporting a molecule through a mammalian barrier membrane of at least one layer of cells comprising the steps of: ablating said membrane with a shear device; and utilizing a driving force to move said molecule through said perforated membrane.

Claims

1. A method for transporting a molecule through a mammal'"'"'s mammalian barrier membrane of at least one layer of cells comprising the steps of:

contacting said mammalian barrier membrane with a sheet of a shear device, the sheet containing at least one opening;

forcing a portion of said mammalian barrier membrane through the at least one opening;

ablating said portion of said mammalian barrier membrane forced through the at least one opening with a shear member of said shear device, wherein the shear member ablates said portion of the mammalian barrier membrane by moving over the sheet and the at least one opening; and

utilizing a driving force to transport said molecule through the ablated portion of the mammalian barrier membrane.

2. A method of claim 1, wherein said shear member is a shear blade.

3. A method of claim 2, wherein said portion of said mammalian barrier membrane is forced through said opening by a pressure force.

4. A method of claim 3, wherein said pressure force is mechanical pressure.

5. A method of claim 3, wherein said pressure force is suction.

6. A method of claim 2, wherein said shear blade moves parallel to said sheet.

7. A method of claim 1, wherein said shear device further comprises a driving unit configured to move said shear member.

8. A method of claim 7, wherein said driving unit is powered manually by a user of the shear device.

9. A method of claim 7, wherein said driving unit is powered by an electric motor of the shear device.

10. A method of claim 7, wherein said shear device further comprises a sensor configured for feedback from said sensor to control said driving unit.

11. A method of claim 10, wherein said sensor is selected from the group consisting of pressure sensor, conductivity sensor, impedance sensor, pH and temperature sensor.

12. A method of claim 11, wherein said sensor is an impedance sensor measuring the impedance of the mammalian barrier membrane.

13. A method of claim 12, wherein the measurements from said impedance sensor are relayed to a microprocessor of the shear device.

14. A method of claim 1, wherein said mammalian barrier membrane is selected from the group consisting of skin, buccal, vaginal, and rectal membranes.

15. A method of claim 1, wherein said mammalian barrier membrane is human skin.

16. A method of claim 1, wherein said driving force is selected from a group consisting of iontophoresis, electro-osmosis, reverse iontophoresis, electroporation, phonophoresis, pressure gradients, and concentration gradients.

17. A method of claim 1, wherein said molecule is a pharmaceutical transported through said mammalian barrier membrane into said mammal.

18. A method of claim 17, wherein said pharmaceutical is selected from the group consisting of polysaccharides, peptides, proteins, and polynucleotides.

19. A method of claim 17, wherein said molecule is a vaccine.

20. A method of claim 19, wherein said molecule is a vaccine against Staphylococcus aureus.

21. A method of claim 1, wherein said molecule is transported from within said mammal out through said mammalian barrier membrane.

22. A method of claim 16, wherein said molecule is glucose.

23. A method of claim 1, wherein said shear member moves parallel to said sheet.

24. A method of claim 1, wherein the area of at least one of said at least one opening is about 0.001 to 5 mm².