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Microfluidic chemostat

  • US 7,407,799 B2
  • Filed: 12/14/2004
  • Issued: 08/05/2008
  • Est. Priority Date: 01/16/2004
  • Status: Expired due to Fees
First Claim
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1. A chemostat chip comprising one or more chemostats, wherein each of the chemostats includes a first compartment adaptable to hold one or more cells and a second compartment adaptable to receive a lysis agent, and wherein the first and second compartments are separated by an actuatable valve that when opened can permit the one or more cells to travel from the first compartment to the second compartment, and when closed can isolate the one or more cells in the second compartment before exposing the second compartment to the lysis agent, whereinsaid chemostat chip further comprises a growth chamber having a plurality of compartments;

  • wherein each of the compartments may be fluidly isolated from the rest of the growth chamber by one or more actuatable valves;

    wherein the compartments of the growth chamber are connected in a loop;

    a nutrient supply-line to supply growth medium to the growth chamber; and

    an output port to remove fluids from the growth chamber.

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