Microfluidic chemostat
First Claim
1. A chemostat chip comprising one or more chemostats, wherein each of the chemostats includes a first compartment adaptable to hold one or more cells and a second compartment adaptable to receive a lysis agent, and wherein the first and second compartments are separated by an actuatable valve that when opened can permit the one or more cells to travel from the first compartment to the second compartment, and when closed can isolate the one or more cells in the second compartment before exposing the second compartment to the lysis agent, whereinsaid chemostat chip further comprises a growth chamber having a plurality of compartments;
- wherein each of the compartments may be fluidly isolated from the rest of the growth chamber by one or more actuatable valves;
wherein the compartments of the growth chamber are connected in a loop;
a nutrient supply-line to supply growth medium to the growth chamber; and
an output port to remove fluids from the growth chamber.
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Accused Products
Abstract
A chemostat is described that includes a growth chamber having a plurality of compartments. Each of the compartments may be fluidly isolated from the rest of the growth chamber by one or more actuatable valves. The chemostat may also include a nutrient supply-line to supply growth medium to the growth chamber, and an output port to remove fluids from the growth chamber. Also, a method of preventing biofilm formation in a growth chamber of a chemostat is described. The method may include the steps of adding a lysis agent to a isolated portion of the growth chamber, and reuniting the isolated portion with the rest of the growth chamber.
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Citations
15 Claims
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1. A chemostat chip comprising one or more chemostats, wherein each of the chemostats includes a first compartment adaptable to hold one or more cells and a second compartment adaptable to receive a lysis agent, and wherein the first and second compartments are separated by an actuatable valve that when opened can permit the one or more cells to travel from the first compartment to the second compartment, and when closed can isolate the one or more cells in the second compartment before exposing the second compartment to the lysis agent, wherein
said chemostat chip further comprises a growth chamber having a plurality of compartments; -
wherein each of the compartments may be fluidly isolated from the rest of the growth chamber by one or more actuatable valves; wherein the compartments of the growth chamber are connected in a loop; a nutrient supply-line to supply growth medium to the growth chamber; and an output port to remove fluids from the growth chamber. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A chemostat chip comprising an array of chemostats, wherein each of the chemostats comprises:
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a growth chamber having a plurality of compartments, wherein each of the compartments may be fluidly isolated from the rest of the growth chamber by one or more actuatable valves to give the growth chamber a variable volume; a nutrient supply-line to supply growth medium to the growth chamber; and an output port to remove fluids from the growth chamber. - View Dependent Claims (14, 15)
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Specification