Adsorbent of high-mobility-group protein and body fluid-purification column

• US 7,408,045 B2
• Filed: 07/21/2006
• Issued: 08/05/2008
• Est. Priority Date: 04/05/2000
• Status: Expired due to Term

• Current Assignee: Toray Industries Incorporated
• Original Assignee: Toray Industries Incorporated
• Inventors: Maruyama, Ikuro, Ida, Nobuo, Masuko, Sanae
• Primary Examiner(s): Lukton; David
• Application Number:
  US11/490,095
• Publication Number:
  US 20060258853A1
• Time in Patent Office: 746 Days
• Field of Search: 530/412, 530/415, 530/417, 604/5.01, 604/5.02, 604/5.03, 604/5.04
• US Class Current: 530/412
• CPC Class Codes: A61M 1/3679 by absorption A61M1/3675 takes precedence
• CPC Class Codes: B01J 20/26 Synthetic macromolecular compounds
• CPC Class Codes: B01J 20/261 obtained by reactions only involving carbon to carbon unsaturated bonds macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds per se C08F
• CPC Class Codes: B01J 20/262 obtained otherwise than by reactions only involving carbon to carbon unsaturated bonds, e.g. obtained by polycondensation macromolecular compounds obtained otherwise than by reactions only involving unsaturated carbon-to-carbon bonds per se C08G
• CPC Class Codes: B01J 20/267 Cross-linked polymers
• CPC Class Codes: B01J 20/28016 Particle form
• CPC Class Codes: B01J 20/28023 Fibres or filaments fibres or filaments in the form of membranes B01J20/28038; B01J20/28007 takes precedence
• CPC Class Codes: B01J 20/321 consisting of a polymer obtained by reactions involving only carbon to carbon unsaturated bonds
• CPC Class Codes: B01J 20/3212 consisting of a polymer obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
• CPC Class Codes: B01J 20/3217 Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
• CPC Class Codes: B01J 20/3219 involving a particular spacer or linking group, e.g. for attaching an active group
• CPC Class Codes: B01J 20/3225 involving a post-treatment of the coated or impregnated product
• CPC Class Codes: B01J 20/3246 having a well defined chemical structure
• CPC Class Codes: B01J 20/3248 the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
• CPC Class Codes: B01J 20/3251 comprising at least two different types of heteroatoms selected from nitrogen, oxygen or sulphur
• CPC Class Codes: B01J 20/3274 Proteins, nucleic acids, polysaccharides, antibodies or antigens
• CPC Class Codes: B01J 2220/58 Use in a single column
• CPC Class Codes: B01J 39/17 containing also inorganic materials, e.g. inert material coated with an ion-exchange resin
• CPC Class Codes: B01J 39/18 Macromolecular compounds B01J39/17 takes precedence
• CPC Class Codes: B01J 41/10 Inorganic material
• CPC Class Codes: B01J 41/12 : Macromolecular compounds

Abstract

An adsorbent of high-mobility-group proteins (HMG protein) which can remove HMG protein in body fluid is disclosed. The adsorbent according to the present invention has a water-insoluble carrier on which (a) substance(s) having (a) hydrogen-bondable functional group(s) and/or (a) hydrophobic functional group(s) is(are) immobilized.

Claims

1. A method for adsorbing high-mobility-group proteins in body fluid, comprising:

contacting an adsorbent with body fluid so as to adsorb said high-mobility-group proteins in said body fluid to said adsorbent;

wherein said adsorbent comprises a water-insoluble carrier on which a substance(s) having a hydrogen-bondable functional group(s) and/or a hydrophobic functional group is(are) immobilized, and

wherein said adsorbent has a rate of adsorption for high-mobility-group proteins of not less than 50%, and has a rate of adsorption for serum albumin of not more than 20% and wherein said body fluid is blood.

2. The method according to claim 1, which is carried out by using a body fluid-purification column for removing high-mobility-group proteins, said body fluid-purification column comprising a column and said absorbent is packed in said column.

3. The method according to claim 1, wherein the body fluid is obtained from a patient that has sepsis.

4. The method according to claim 3, which is carried out by using a body fluid-purification column for removing high-mobility-group proteins, said body fluid- purification column comprising a column and said absorbent is packed in said column, together with a body fluid-purification column(s) which adsorbs a substance(s) originating from bacteria.

5. The method according to claim 2, wherein extracorporcal circulation of whole blood is attained by said body fluid-purification column step.

6. The method according to claim 1, wherein said hydrogen-bondable functional group(s) is(are) a cationic functional group(s).

7. The method according to claim 6, wherein said cationic functional group(s) is(are) at least one selected from the group consisting of a primary amino group, secondary amino group, tertiary amino group, imino group and/or quaternary ammonium group.

8. The method according to claim 6, wherein said cationic functional group(s) is(are) represented by the following formula (I):

9. The mcthod according to claim 8, wherein each of R¹, R²and R³independently represents a hydrogen atom or a C₁-C₂alkyl group.

10. The method according to claim 8, wherein each of R¹, R²and R³independently represents a methyl group.

11. The method according to claim 1, wherein said hydrogen-bondable functional group(s) is(are) an anionic functional group(s).

12. The method according to claim 11, wherein said hydrophobic functional group(s) is(are) at least one selected from the group consisting of a carboxyl group, sulfate group, sulfonic acid group and phosphate group.

13. The method according to claim 1, wherein said hydrophobic functional group(s) is(are) an alkyl group(s) having not less than 6carbon atoms or an aromatic group(s).

14. The method according to claim 1, wherein said substance(s) having the hydrogen-bondable functional group(s) and/or a hydrophobic functional group(s) is(are) a peptide(s) or an amino acid(s).

15. The method according to claim 7, wherein said substance(s) having the hydrogen-bondable functional group(s) is(are) a peptide(s) or an amino acid(s) having an amino group(s) in its(their) side chain(s).

16. The method according, to claim 15, wherein said substance having the hydrogen-bondable functional group(s) is(are) polylysine.

17. The method according to claim 11, wherein a polysaccharide(s) having a sulfate group(s) is(are) immobilized.

18. The method according to claim 17, wherein said polysaccharide is heparin or dextran sulfate, or a derivative thereof.

19. The method according to claim 1, wherein said adsorbent comprises the water-insoluble carrier on which an antibody(ies) to said high-mobility-group proteins is(are) immobilized.

20. The method according to claim 19, wherein said adsorbent has a rate of adsorption for high-mobility-group proteins of not less than 50%, and has a rate of adsorption for serum albumin of not more than 20%.

21. The method according to claim 1 or 19, wherein said water-insoluble carrier is in the form of fibers.

22. The method according to claim 1 or 19, wherein said water-insoluble carrier is in the form of beads.