25869, 25934, 26335, 50365, 21117, 38692, 46508, 16816, 16839, 49937, 49931 and 49933 molecules and uses therefor

• US 7,411,054 B2
• Filed: 06/02/2006
• Issued: 08/12/2008
• Est. Priority Date: 03/07/2000
• Status: Expired due to Fees

• Current Assignee: Millennium Pharmaceuticals Incorporated (Takeda Pharmaceutical Company Limited)
• Original Assignee: Millennium Pharmaceuticals Incorporated (Takeda Pharmaceutical Company Limited)
• Inventors: Meyers, Rachel E., Williamson, Mark J.
• Primary Examiner(s): Nashed; Nashaat T.
• Assistant Examiner(s): Moore; William W.
• Application Number:
  US11/445,606
• Publication Number:
  US 20070059730A1
• Time in Patent Office: 802 Days
• Field of Search: None
• US Class Current: 536/23.2
• CPC Class Codes: A01K 2217/05 Animals comprising random inserted nucleic acids (transgenic)
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/395 from Saccharomyces
• CPC Class Codes: C07K 14/43545 from Caenorhabditis
• CPC Class Codes: C07K 14/43581 from Drosophila
• CPC Class Codes: C07K 14/43595 from coelenteratae, e.g. medusae
• CPC Class Codes: C07K 14/463 from amphibians
• CPC Class Codes: C07K 14/465 from birds
• CPC Class Codes: C07K 14/47 from mammals
• CPC Class Codes: C07K 14/705 Receptors; Cell surface antigens; Cell surface determinants tumour specific antigens C07K14/4748
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C12N 2310/3513 Protein; Peptide
• CPC Class Codes: C12N 9/00 Enzymes; Proenzymes; Compositions thereof preparations containing enzymes for cleaning teeth A61K8/66, A61Q11/00; medicinal preparations containing enzymes or proenzymes A61K38/43; enzyme containing detergent compositions C11D; enzymes with nucleic acid structure, e.g. ribozymes, C12N15/113; Processes for preparing, activating, inhibiting, separating or purifying enzymes preparation of malt C12C1/00
• CPC Class Codes: C12N 9/0071 acting on paired donors with incorporation of molecular oxygen (1.14)
• CPC Class Codes: C12N 9/0083 Miscellaneous (1.14.99)
• CPC Class Codes: C12N 9/1205 Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
• CPC Class Codes: C12N 9/18 Carboxylic ester hydrolases (3.1.1)
• CPC Class Codes: C12N 9/20 Triglyceride splitting, e.g. by means of lipase
• CPC Class Codes: C12N 9/88 Lyases (4.)
• CPC Class Codes: C12Y 207/01001 Hexokinase (2.7.1.1)
• CPC Class Codes: C12Y 301/04003 : Phospholipase C (3.1.4.3)

Abstract

The invention provides isolated nucleic acids molecules, designated 25869, 25934, 26335, 50365, 21117, 38692, 46508, 16816, 16839, 49937, 49931 and 49933 nucleic acid molecules. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing 25869, 25934, 26335, 50365, 21117, 38692, 46508, 16816, 16839, 49937, 49931 or 49933 nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a 25869, 25934, 26335, 50365, 21117, 38692, 46508, 16816, 16839, 49937, 49931 or 49933 gene has been introduced or disrupted. The invention still further provides isolated 25869, 25934, 26335, 50365, 21117, 38692, 46508, 16816, 16839, 49937, 49931 or 49933 proteins, fusion proteins, antigenic peptides and anti-25869, 25934, 26335, 50365, 21117, 38692, 46508, 16816, 16839, 49937, 49931 or 49933 antibodies. Diagnostic and therapeutic methods utilizing compositions of the invention are also provided.

Claims

1. An isolated nucleic acid selected from the group consisting of:

a) a nucleic acid comprising the nucleotide sequence of SEQ ID NO;

20 or SEQ ID NO;

22;

b) a nucleic acid which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO;

21;

c) a nucleic acid comprising a nucleotide sequence which is at least 95% identical to the nucleotide sequence of SEQ ID NO;

20 or SEQ ID NO;

22, wherein the nucleotide sequence encodes a polypeptide which has hexokinase activity; and

d) a nucleic acid which encodes a polypeptide comprising an amino acid sequence which is at least 95% identical to the amino acid sequence of SEQ ID NO;

21, wherein the polypeptide has hexokinase activity.

2. The nucleic acid of claim 1, wherein the nucleic acid encodes a polypeptide comprising an amino acid sequence which is at least 95% identical to the amino acid sequence of SEQ ID NO:

21, wherein the polypeptide has hexokinase activity.

3. The nucleic acid of claim 1, wherein the nucleic acid comprises a nucleotide sequence which is at least 95% identical to the nucleotide sequence of SEQ ID NO:

20 or SEQ ID NO;

22, wherein the nucleotide sequence encodes a polypeptide which has hexokinase activity.

4. The nucleic acid of claim 1, wherein the nucleic acid comprises the nucleotide sequence of SEQ ID NO:

20 or SEQ ID NO;

22.

5. The nucleic acid of claim 1, wherein the nucleic acid encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:

21.

6. The nucleic acid of claim 1, wherein the nucleic acid consists of the nucleotide sequence of SEQ ID NO:

20 or SEQ ID NO;

22.

7. The nucleic acid of claim 1, wherein the nucleic acid consists of a nucleotide sequence which encodes the amino acid sequence of SEQ ID NO:

21.

8. A vector comprising the nucleic acid of claim 1.

9. A vector comprising the nucleic acid of claim 2.

10. A vector comprising the nucleic acid of claim 3.

11. An isolated host cell comprising the nucleic acid of claim 1.

12. An isolated host cell comprising the nucleic acid of claim 2.

13. An isolated host cell comprising the nucleic acid of claim 3.

14. A method for producing a polypeptide selected from the group consisting of:

a) a polypeptide comprising the amino acid sequence of SEQ ID NO;

21;

b) a polypeptide encoded by a nucleic acid comprising the nucleotide sequence of SEQ ID NO;

20 or SEQ ID NO122;

c) a polypeptide comprising an amino acid sequence which is at least 95% identical to the amino acid sequence of SEQ ID NO;

21, wherein the polypeptide has hexokinase activity; and

d) a polypeptide encoded by a nucleic acid comprising a nucleotide sequence which is at least 95% identical to the nucleotide sequence of SEQ ID NO;

20 or SEQ ID NO;

22, wherein the polypeptide has hexokinase activity;

the method comprising culturing the host cell of claim 11 under conditions in which the polypeptide is expressed, whereby the polypeptide is produced.