Method of synthesizing polynucleotides using ionic liquids
First Claim
Patent Images
1. A method of forming an internucleotide bond comprising contacting a free hydroxyl of a growing polynucleotide with a solution comprising a nucleotide monomer and at least 50% by weight of an ionic liquid.
0 Assignments
0 Petitions
Accused Products
Abstract
A method of synthesizing polynucleotides is disclosed. The method involves contacting a first nucleotide with a selected reactive group in the presence of an ionic liquid. The selected reactive group may be on a second nucleotide, a polynucleotide, or on a moiety on an insoluble substrate, for example in an oligonucleotide synthesizer.
65 Citations
24 Claims
- 1. A method of forming an internucleotide bond comprising contacting a free hydroxyl of a growing polynucleotide with a solution comprising a nucleotide monomer and at least 50% by weight of an ionic liquid.
-
12. A method of forming an intemucleotide bond between a first nucleoside moiety and a second nucleoside moiety, the method comprising
(a) dissolving the first nucleoside moiety in a solution comprising at least 50% by weight of an ionic liquid, and (b) contacting the second nucleoside moiety with the product of (a) to form the internucleotide bond.
Specification