Augmentation and repair of vocal cord tissue defects

• US 7,412,978 B1
• Filed: 08/08/2000
• Issued: 08/19/2008
• Est. Priority Date: 02/20/1997
• Status: Expired due to Fees

• Current Assignee: Castle Creek Biosciences LLC (Castle Creek Biosciences, Inc.)
• Original Assignee: Isolagen Technologies Incorporated
• Inventors: Keller, Gregory S.
• Primary Examiner(s): Matthews; William H.
• Application Number:
  US09/634,038
• Time in Patent Office: 2,933 Days
• Field of Search: 128/898, 623/11.11, 623/14.11, 623/23.72, 623/23.76, 623/66.1, 623/902, 623/915, 424/93.7
• US Class Current: 128/898
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61L 27/24 Collagen
• CPC Class Codes: A61L 27/3633 Extracellular matrix [ECM]
• CPC Class Codes: A61L 27/3641 characterised by the site of application in the body materials for artificial blood vessels A61L27/507; materials for use in artificial skin A61L27/60
• CPC Class Codes: A61L 27/3683 subjected to a specific treatment prior to implantation, e.g. decellularising, demineralising, grinding, cellular disruption/non-collagenous protein removal, anti-calcification, crosslinking, supercritical fluid extraction, enzyme treatment
• CPC Class Codes: A61L 27/3804 characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
• CPC Class Codes: A61L 27/3839 characterised by the site of application in the body materials for artificial blood vessels A61L27/507; materials for use in artificial skin A61L27/60
• CPC Class Codes: A61L 27/3895 using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
• CPC Class Codes: A61P 11/04 for throat disorders
• CPC Class Codes: C12N 2533/54 Collagen; Gelatin
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: C12N 5/0653 Adipocytes; Adipose tissue
• CPC Class Codes: C12N 5/0656 Adult fibroblasts

Abstract

A method for corrective surgery in a human subject of a vocal cord defect amenable to rectification by the augmentation of tissue subadjacent to the vocal cord defect, the method comprising: placing an effective amount of autologous in vitro cultured cells into a vocal cord tissue of the subject in a position subadjacent to the vocal cord defect, wherein the vocal cord tissue is selected from the group consisting of a scar, Reinke'"'"'s space, a muscle of the vocal cord, and the lamina propria, wherein the in vitro cultured cells are obtained by culturing a plurality of viable cells retrieved from the subject, and wherein the cultured cells are suspended in a collagen or modified collagen solution prior to injection of the cultured cells. The in vitro cultured cells can be fibroblasts, e.g., fibroblasts derived from dermis, fascia, connective tissue, or lamina propria.

Claims

1. A method for corrective surgery in a human subject of a vocal cord defect amenable to rectification by the augmentation of tissue subadjacent to the vocal cord defect, the method comprising:

placing an effective amount of autologous in vitro cultured cells into a vocal cord tissue of the subject in a position subadjacent to the vocal cord defect,

wherein the vocal cord tissue is selected from the group consisting of a scar, Reinke'"'"'s space, a muscle of the vocal cord, and the lamina propria,

wherein the in vitro cultured cells are obtained by culturing a plurality of viable cells retrieved from the subject, and

wherein the cultured cells are suspended in a collagen or modified collagen solution prior to injection of the cultured cells.

2. The method of claim 1 wherein the cells are cultured in a collagen or modified collagen matrix.

3. The method of claim 2 wherein the cells and the matrix are placed in the tissue by injection.

4. The method of claim 2 wherein the cells and the matrix are placed in the tissue by engraftment.

5. The method of claim 1 wherein the in vitro cultured cells are fibroblasts.

6. The method of claim 5 wherein the fibroblasts are derived from a tissue selected from the group consisting of dermis, fascia, connective tissue, and lamina propria.

7. The method of claim 1 wherein serum from the subject is used for the in vitro culture of the cells.