Compositions and methods for detecting and treating diseases and conditions related to chemokine receptors

• US 7,413,866 B2
• Filed: 05/30/2003
• Issued: 08/19/2008
• Est. Priority Date: 11/30/2001
• Status: Active Grant

• Current Assignee: ChemoCentryx, Inc. (Amgen Inc.)
• Original Assignee: ChemoCentryx, Inc. (Amgen Inc.)
• Inventors: Schall, Thomas J., Wei, Zheng, Burns, Jennifer M., Howard, Maureen C., Miao, Zhenhua, Premack, Brett A.
• Primary Examiner(s): Allen; Marianne P.
• Assistant Examiner(s): DeBerry; Regina M
• Application Number:
  US10/452,015
• Publication Number:
  US 20040018563A1
• Time in Patent Office: 1,908 Days
• Field of Search: None
• US Class Current: 435/7.2
• CPC Class Codes: G01N 2500/10 involving cells
• CPC Class Codes: G01N 33/6863 Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors

Abstract

The present invention provides methods for identifying an agent that specifically binds and/or modulates one topology of a chemokine receptor but not a second topology of the receptor. Such agents are useful as therapeutics for diseases or conditions associated with a particular chemokine receptor topology. Moreover, the agents are useful for detecting a particular topology of a chemokine receptor, thereby diagnosing disease or predisposition for a disease. In addition, the agents are useful for identifying and isolating cells that express a particular topology of a chemokine receptor.

Claims

1. A method of identifying a cancer cell having a chemokine receptor, wherein the cell is from a tissue, the method comprising

contacting the cell with I-TAC and SDF-1;

and

determining whether I-TAC and SDF-1 compete with each other for binding on the chemokine receptor, wherein competition for binding indicates the presence of a cancer cell.

2. The method of claim 1, wherein the identified cancer cell is selected from the group consisting of a breast cancer cell, an ovarian cancer cell, cervical cancer cell, a Burkitt'"'"'s lymphoma cell and a glioblastoma cell.