Methods for degrading or converting plant cell wall polysaccharides
First Claim
1. A method for degrading or converting plant cell wall polysaccharides into glucose, comprising:
- treating the plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant Trichoderma reesei cell comprising a heterologous polynucleotide encoding the beta-glucosidase of SEQ ID NO;
27, wherein the Trichoderma reesei cell comprises one or more native genes encoding enzymes that degrade or convert plant cell wall polysaccharides and the spent whole fermentation broth degrades or converts the plant cell wall polysaccharides into glucose.
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Abstract
The present invention relates to methods for converting plant cell wall polysaccharides into one or more products, comprising: treating the plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into the one or more products. The present invention also relates to methods for producing an organic substance, comprising: (a) saccharifying plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into saccharified material; (b) fermenting the saccharified material of step (a) with one or more fermenting microoganisms; and (c) recovering the organic substance from the fermentation.
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Citations
9 Claims
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1. A method for degrading or converting plant cell wall polysaccharides into glucose, comprising:
- treating the plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant Trichoderma reesei cell comprising a heterologous polynucleotide encoding the beta-glucosidase of SEQ ID NO;
27, wherein the Trichoderma reesei cell comprises one or more native genes encoding enzymes that degrade or convert plant cell wall polysaccharides and the spent whole fermentation broth degrades or converts the plant cell wall polysaccharides into glucose. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
- treating the plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant Trichoderma reesei cell comprising a heterologous polynucleotide encoding the beta-glucosidase of SEQ ID NO;
Specification