Immortalized fibroblasts

• US 7,413,900 B2
• Filed: 10/31/2005
• Issued: 08/19/2008
• Est. Priority Date: 10/31/2005
• Status: Expired due to Fees

• Current Assignee: Centro De Investigacion Y De Estudios Avanzados Del I.P.N., President and Fellows of Harvard College (Harvard Corporation)
• Original Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Inventors: Green, Howard, Marsch-Moreno, Meytha, Kuri-Harcuch, Walid, Iuchi, Shiro
• Primary Examiner(s): Nguyen; Quang
• Application Number:
  US11/263,549
• Publication Number:
  US 20070098696A1
• Time in Patent Office: 1,023 Days
• Field of Search: None
• US Class Current: 435/357
• CPC Class Codes: A61K 35/48 Reproductive organs
• CPC Class Codes: C12N 2502/02 embryonic cells
• CPC Class Codes: C12N 2502/13 connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
• CPC Class Codes: C12N 2510/04 Immortalised cells
• CPC Class Codes: C12N 5/0606 Pluripotent embryonic cells, e.g. embryonic stem cells [ES] embryonic germ cells C12N5/0611, induced pluripotent stem cells C12N5/0696
• CPC Class Codes: C12N 5/0652 Cells of skeletal and connective tissues; Mesenchyme

Abstract

Aspects of the invention provide an immortalized fibroblast cell line capable of the growth and maintenance of human embryonic stem cells. An immortalized fibroblast cell line derived from an early stage mouse embryo is provided. Methods of preparing an immortalized fibroblast cell line from an early stage mouse embryo are also provided.

Claims

1. A composition comprising MMM cells, or progeny thereof, deposited with the ATCC under accession number PTA-7190.