Modified green fluorescent proteins and methods for using same
First Claim
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1. An isolated nucleic acid, comprising:
- a nucleic acid sequence encoding a genetically engineered fluorescent protein that is substantially identical to and has at least 97% identity with the amino acid sequence selected from the group consisting of SEQ ID NO;
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Abstract
The present invention provides nucleic acid molecules encoding mutant fluorescent proteins as well as proteins encoded by these nucleic acids. In addition, host-cells, stable cell lines and transgenic organisms comprising the above-referenced nucleic acid molecules are provided. The subject protein and nucleic acid compositions find use in a variety of different applications and methods, particularly for labeling of biomolecules, cells, or cell organelles.
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17 Claims
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1. An isolated nucleic acid, comprising:
a nucleic acid sequence encoding a genetically engineered fluorescent protein that is substantially identical to and has at least 97% identity with the amino acid sequence selected from the group consisting of SEQ ID NO;
18.- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. An isolated nucleic acid, comprising:
a nucleic acid sequence encoding a genetically engineered fluorescent protein with the amino acid sequence of SEQ ID NO;
18.
Specification