PNA chip for determining genotypes of mycobacterial species using plastic substrate coated with epoxy group-containing polymer and method of determining genotypes of mycobacterial species using the PNA chip
First Claim
1. A peptide nucleic acid (PNA) chip for determining the genotypes of mycobacterial species wherein a plurality of probe PNAs is immobilized on a plastic substrate coated with an epoxy group-containing polymer,wherein the plurality of probe PNAs comprisesa probe PNA consisting of SEQ ID NO:
- 5,a probe PNA consisting of SEQ ID NO;
6, anda probe PNA consisting of a sequence selected from the group consisting of SEQ ID NOs;
7-28,wherein each probe PNA is immobilized in an individual spot; and
wherein the mycobacterial species are selected from the group consisting of M. tuberculosis complex, MAC complex, M. fortuitum, M. chelonae, M. abscessus, M. kansasii, M. gordonae, M. scrofulaceum, M. szulgai, M. vaccae, M. xenopi, M. terrae, M. flavescens, M. smegmatis, M. malmoense, M. simiae, M. marinum-ulcerance, M. gastri, M. avium, M. intracellulae, M. genavense, and M. lentiflavum.
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Accused Products
Abstract
Provided are a PNA chip for determining the genotypes of mycobacterial species in which probe PNAs having DNA sequences of SEQ ID NOs: 5-28 are immobilized on a plastic substrate coated with an epoxy group-containing polymer, and a method of determining the genotypes of mycobacterial species using the PNA chip. According to the PNA chip and the method of determining the genotypes of mycobacterial species, the genotypes of mycobacterial species which are causes of tuberculosis and respiratory diseases can be determined within a short term with good sensitivity and specificity. Therefore, detection of the presence of mycobacterial species in test samples, and after confirmation of the presence of mycobacterial species, identification of the mycobacterial species and determination of whether or not the mycobacterial species are tuberculosis-causing mycobacterial species can be achieved promptly and accurately.
3 Citations
13 Claims
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1. A peptide nucleic acid (PNA) chip for determining the genotypes of mycobacterial species wherein a plurality of probe PNAs is immobilized on a plastic substrate coated with an epoxy group-containing polymer,
wherein the plurality of probe PNAs comprises a probe PNA consisting of SEQ ID NO: - 5,
a probe PNA consisting of SEQ ID NO;
6, anda probe PNA consisting of a sequence selected from the group consisting of SEQ ID NOs;
7-28,wherein each probe PNA is immobilized in an individual spot; and wherein the mycobacterial species are selected from the group consisting of M. tuberculosis complex, MAC complex, M. fortuitum, M. chelonae, M. abscessus, M. kansasii, M. gordonae, M. scrofulaceum, M. szulgai, M. vaccae, M. xenopi, M. terrae, M. flavescens, M. smegmatis, M. malmoense, M. simiae, M. marinum-ulcerance, M. gastri, M. avium, M. intracellulae, M. genavense, and M. lentiflavum. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
- 5,
Specification