Mass spectrometric methods for detecting mutations in a target nucleic acid
DCFirst Claim
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1. A process for detecting one or more target nucleotide sequences in a biological sample, comprising:
- a) amplifying a target nucleic acid molecule, wherein the amplification product is a natural nucleic acid;
b) ionizing and volatilizing the product of step (a); and
c) analyzing the product of step (b) by mass spectrometry, wherein detection of the target nucleotide sequence by mass spectrometry indicates the presence of the target nucleotide sequence in the biological sample.
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Abstract
Provided herein in certain embodiments are processes that generally include the following steps: amplifying a target nucleic acid molecule; ionizing and volatilizing the amplification product and analyzing the ionized and volatilized product by mass spectrometry. In some embodiments the amplification product is a natural nucleic acid and detection of the target nucleotide sequence by mass spectrometry indicates the presence of the target nucleotide sequence in the biological sample.
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12 Claims
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1. A process for detecting one or more target nucleotide sequences in a biological sample, comprising:
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a) amplifying a target nucleic acid molecule, wherein the amplification product is a natural nucleic acid; b) ionizing and volatilizing the product of step (a); and c) analyzing the product of step (b) by mass spectrometry, wherein detection of the target nucleotide sequence by mass spectrometry indicates the presence of the target nucleotide sequence in the biological sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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Specification