Method of making a test device
First Claim
1. A method for making a test device for detecting or quantifying an analyte in a sample comprising:
- providing a membrane with a sugar glaze on its outer surface,contacting the sugar glazed membrane with a mixture comprising derivatized, marker-loaded liposomes,substantially dehydrating the mixture on the membrane under vacuum pressure at a temperature of from about 4°
C. to about 80°
C., andimmobilizing a first binding material specific for the analyte in a capture zone on the membrane.
0 Assignments
0 Petitions
Accused Products
Abstract
The present invention relates to a method for making a test device for detecting or quantifying an analyte in a sample. This method involves contacting a membrane with a mixture including derivatized, marker-loaded liposomes, and substantially dehydrating the mixture on the membrane under vacuum pressure at a temperature of from about 4° C. to about 80° C., wherein said mixture further includes one or more sugars in an amount sufficient to promote the stability of the liposomes during dehydration and rehydration. The present invention also relates to a test device and method for detecting or quantifying an analyte in a sample. The test device includes a membrane which includes an immobilized liposome zone, wherein the immobilized liposome zone has bound thereto dehydrated, derivatized, marker-loaded liposomes dehydrated under vacuum pressure at a temperature of from about 4° C. to about 80° C. from a mixture which includes one or more sugars in an amount sufficient to promote the stability of the liposomes during dehydration and rehydration.
-
Citations
15 Claims
-
1. A method for making a test device for detecting or quantifying an analyte in a sample comprising:
-
providing a membrane with a sugar glaze on its outer surface, contacting the sugar glazed membrane with a mixture comprising derivatized, marker-loaded liposomes, substantially dehydrating the mixture on the membrane under vacuum pressure at a temperature of from about 4°
C. to about 80°
C., andimmobilizing a first binding material specific for the analyte in a capture zone on the membrane. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
-
-
10. A test device for detecting or quantifying an analyte in a sample comprising:
a sugar glazed membrane which comprises an immobilized liposome zone, wherein the immobilized liposome zone has bound thereto dehydrated, derivatized, marker-loaded liposomes and a capture zone having a first binding material specific for the analyte bound thereto. - View Dependent Claims (11, 12, 13, 14, 15)
Specification