Transfer sheet for transferring biologically active substance to culture plate

• US 7,419,820 B2
• Filed: 12/16/2004
• Issued: 09/02/2008
• Est. Priority Date: 12/16/2003
• Status: Expired due to Fees

• Current Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Original Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Inventors: Miyazaki, Takeshi, Kanome, Osamu, Watanabe, Kohei, Fujiyama, Tomoyo, Matsuda, Ryoichi
• Primary Examiner(s): Weber; Jon P
• Assistant Examiner(s): Shen; Bin
• Application Number:
  US10/554,211
• Publication Number:
  US 20060246508A1
• Time in Patent Office: 1,356 Days
• Field of Search: None
• US Class Current: 435/287.6
• CPC Class Codes: B01L 2300/0812 Bands; Tapes
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/123 Flexible; Elastomeric
• CPC Class Codes: B01L 3/0244 using pins
• CPC Class Codes: B01L 3/0262 using touch-off at substrate or container
• CPC Class Codes: B01L 3/0293 for liquids
• CPC Class Codes: B01L 3/50853 with covers or lids
• CPC Class Codes: C12M 23/12 Well or multiwell plates C12M25/04 takes precedence
• CPC Class Codes: C12M 23/38 Caps; Covers; Plugs; Pouring means
• CPC Class Codes: C12M 29/04 Filters; Permeable or porous membranes or plates, e.g. dialysis
• CPC Class Codes: C12Q 1/02 involving viable microorganisms
• CPC Class Codes: G01N 33/5005 involving human or animal cells immunoassay G01N33/56966; immunoassays of protozoa G01N33/56905; protozoa in screening assays C12Q1/025

Abstract

The invention provides a biologically active substance transfer sheet which can be formed with simple steps, a cell culture kit which comprises a cell culture plate and a biologically active substance transfer sheet, a producing method therefor, and a method for screening cell culture conditions with cells. The transfer sheet is prepared by providing biologically active substances having biological activity in plural areas on a sheet base, and biologically active substances are supplied from the sheet to culture regions provided on the plate.

Claims

1. An elastic transfer sheet that transfers a biologically active substance that affects a function of a cell to a culture region on a culture plate when placed on the culture plate, the elastic transfer sheet comprising:

a sheet base; and

two or more holding areas provided on the sheet base, each of the two or more holding areas holding a biologically active substance,

wherein each of the two or more holding areas is provided in a position for covering a culture region of the culture plate,

wherein the biologically active substance is releasable from the elastic transfer sheet, and

wherein each of the two or more holding areas is surrounded by its own protruding wall structure.

2. The transfer sheet according to claim 1, wherein the two or more holding areas hold different biologically active substances or different combinations of two or more biologically active substances.

3. The transfer sheet according to claim 1, wherein the two or more holding areas hold a biologically active substance in different concentrations.

4. The transfer sheet according to claim 1, wherein each of the two or more holding areas is able to release the biologically active substance in a sustainable manner.

5. The transfer sheet according to claim 1, wherein each of the two or more holding areas is a protruding area provided on the sheet base, and the biologically active substance is held on the protruding area.

6. A method for applying a biologically active substance to the transfer sheet according to claim 1, the method comprising a step of providing each of the two or more holding areas of the transfer sheet with the biologically active substance by using liquid discharge means.

7. The method according to claim 6, wherein the liquid discharge means is discharge means by a thermal ink jet means.

8. The method according to claim 6, wherein the liquid discharge means is discharge means by a piezo ink jet means.

9. The method according to claim 6, further comprising a step of immobilizing the biologically active substance by applying an immobilizing energy from the exterior.