Method and apparatus for extended hyperspectral imaging

• US 7,420,679 B2
• Filed: 09/20/2005
• Issued: 09/02/2008
• Est. Priority Date: 06/30/2004
• Status: Active Grant

• Current Assignee: Chemimage Corporation
• Original Assignee: Chemimage Corporation
• Inventors: Nelson, Matthew P., Gardner, Charles W. Jr., Treado, Patrick J.
• Primary Examiner(s): Evans; F. L.
• Assistant Examiner(s): Nur; Abdullahi
• Application Number:
  US11/229,539
• Publication Number:
  US 20060050278A1
• Time in Patent Office: 1,078 Days
• Field of Search: 356/417, 356/301, 356/303, 356/317, 356/320, 356/326, 600/160, 600/476, 250/458.1, 250/461.2, 250/459.1, 250/461.1, 250/462.1, 250/266, 359/355, 359/356, 359/357, 359/361, 359/368, 359/372, 359/373, 359/374, 359/385, 359/388, 359/389, 359/363
• US Class Current: 356/419
• CPC Class Codes: G01N 21/255 Details, e.g. use of specially adapted sources, lighting or optical systems

Abstract

The disclosure relates to method and apparatus for obtaining a multimodal hyperspectral image of a sample for widefield spectral analysis. An apparatus according to one embodiment may include a plurality of optical lenses configured to interchangeably receive photons and focus the photons; a plurality of tunable filters positioned in a filter housing, each tunable filter selectively receiving the focused dispersed photons from one of the plurality of optical lenses, each tunable filter providing wavelength-selective filtered photons; a first optical camera and a second optical cameras for selectively receiving the wavelength-selective filtered photons from each of the plurality of tunable filters and combining said filtered photons to form a hyperspectral spatially accurate, wavelength-resolved image of the sample.

Claims

1. A multimodal hyperspectral imaging system for widefield spectral analysis of a sample comprising:

a first optical lens and a second optical lens configured to interchangeably receive photons from said sample and to focus said photons;

a plurality of tunable filters positioned in a filter housing, each of said plurality of tunable filters configured to selectively receive said focused photons from either said first optical lens or from said second optical lens and to provide wavelength-selective filtered photons;

a first optical camera and a second optical camera, each configured to selectively receive said wavelength-selective filtered photons from a predetermined one of said plurality of tunable filters and to combine said wavelength-selective filtered photons to form a hyperspectral spatially accurate wavelength-resolved image of the sample.

2. The imaging system of claim 1, wherein said photons from said sample are selected from the group consisting of photons reflected by said sample, photons refracted by said sample, luminescence, fluorescence, photons Raman scattered by said sample, photons transmitted by said sample, photons adsorbed by said sample, and photons emitted by said sample.

3. The imaging system of claim 1, further comprising an optical mirror for selectively communicating photons from either said first optical lens or said second optical lens to a predetermined one of said plurality of the optical filters.

4. The imaging system of claim 1, wherein said filter housing is adapted to optically couple each of said first or second optical lenses with a predetermined selective one or combination of said plurality of optical filters.

5. The imaging system of claim 1, wherein said first optical lens is a NIR zoom optics or a reflective optics.

6. The imaging system of claim 1, wherein said second optical lens is a UV-VIS zoom optics or a reflective optics.

7. The imaging system of claim 1, wherein at least one of said first optical lens and said second optical lens defines an optical train.

8. The imaging system of claim 1, wherein said first optical lens and said second optical lens are further configured to simultaneously receive photons from said sample.

9. The imaging system of claim 1, wherein said first optical lens and said second optical lens are configured to sequentially receive photons from the sample.

10. The imaging system of claim 1, wherein at least one of said tunable filters is selected from the group consisting of an LCTF, an AOTF, a dielectric filter, a rotary filter and a linear variable filter.

11. The imaging system of claim 1, wherein at least one of said tunable filters is selected from the group consisting of a VIS LCTF, a SW-NIR LCTF, a MW-NIR LCTF, a LW-NIR LCTF and a UV-LCTF.

12. The imaging system of claim 1, wherein said first optical camera is selected from the group consisting of a charged couple device and a focal array device.

13. The imaging system of claim 1, wherein said second optical camera is selected from the group consisting of an InGaAs camera, an InSb camera and an MCT camera.

14. The imaging system of claim 1, wherein said second optical camera is an InSb camera.

15. The imaging system of claim 1, wherein said widefield spectral analysis is collected over a wavelength range of 250-2500 nm.

16. The imaging system of claim 1, wherein said multimodal hyperspectral imaging system is an integrated imaging device.

17. The imaging system of claim 1, wherein said hyperspectral image is a spatially accurate wavelength-resolved image of said sample in a wavelength range of 250-2500 nm.

18. The imaging system of claim 1, wherein said hyperspectral image is a spatially accurate wavelength-resolved image of said sample in a wavelength range of 250-1700 nm.

19. A method for obtaining a hyperspectral image of a sample using an integrated imaging device, the method comprising:

illuminating said sample with a plurality of photons to produce photons from said sample;

providing a first optical lens and a second optical lens for independently collecting and focusing said photons from said sample, said first optical lens and said second optical lens thereby providing focused photons of a first wavelength and a second wavelength, respectively;

selectively directing said focused photons having a first wavelength to one of a plurality of optical filters and said focused photons having a second wavelength to a second of said plurality of optical filters, each of said plurality of optical filters providing a set of filtered photons of a predetermined wavelength;

selectively directing each set of filtered photons of a predetermined wavelength to either a first photon detector or a second photon detector, said first photon detector and said second photon detector independently forming a spatially accurate wavelength-resolved image of the sample from said set of filtered photons of a predetermined wavelength.

20. The method of claim 19, wherein said photons from said sample are selected from the group consisting of photons reflected by said sample, photons refracted by said sample, luminescence, fluorescence, Raman scattered photons, transmitted by said sample, photons adsorbed by said sample, and photons emitted by said sample.

21. The method of claim 19, further comprising combining said spatially accurate wavelength-resolved images of said sample from said first optical detector and said second optical detector to form a hyperspectral image of said sample.

22. The method of claim 19, wherein said step of obtaining a hyperspectral image of said sample includes combining said set of filtered photons provided by said first optical filter with said set of filtered photons provided by said second optical filter.

23. The method of claim 19, wherein said hyperspectral image is a spatially accurate wavelength-resolved image of said sample in a wavelength range of 250-2500 nm.

24. The method of claim 19, wherein said hyperspectral image is a spatially accurate wavelength-resolved image of said sample in a wavelength range of 250-1700 nm.

25. The method of claim 19, wherein said step of selectively directing said focused photons to one of a plurality of optical filters includes optically connecting one of said first and second optical lenses to one of said plurality of optical filters, wherein said optical lens and said optical filter have an overlapping operating wavelength.

26. The method of claim 19, wherein said step of selectively directing each set of filtered photons of a predetermined wavelength to a first photon detector or a second photon detector includes optically connecting one of said plurality of optical filters to one of said photon detectors, wherein said optical filter and said photon detector have an overlapping operating wavelength.

27. The method of claim 19, wherein said first optical lens is a NIR zoom optics.

28. The method of claim 19, wherein said second optical lens is a UV-VIS zoom optics.

29. The method of claim 19, wherein at least one of said first optical lens and said second optical lens defines an optical train.

30. The method of claim 19, wherein at least one of said plurality of filters is a liquid crystal tunable filer.

31. The method of claim 19, wherein said first optical lens and said second optical lens are configured to simultaneously receive photons from said sample.

32. The method of claim 19, wherein said step of illuminating said sample includes illuminating said sample with photons having wavelengths in the range of 250-2500 nm.

33. The method of claim 19, wherein said step of illuminating said sample includes illuminating said sample with photons having wavelengths in the range of 250-1700 nm.

34. The method of claim 19, wherein said first photon detector is a silicon CCD.

35. The method of claim 19, wherein said second photon detector is an InGaAs camera.

36. The method of claim 19, wherein said second photon detector is an InSb camera.

37. The method of claim 19, wherein said step of selectively directing said focused photons to a plurality of optical filters includes using a mirror to selectively direct said focused photons from said first optical lens to at least one of said plurality of optical filters.

38. The method of claim 19, wherein said step of selectively directing said filtered photons to said first photon detector includes using a mirror to selectively direct said filtered photons from said first optical filter to said first photon detector.

39. A system for collecting a hyperspectral image of a sample comprising:

a plurality of optical lenses for receiving photons from said sample and forming focused photons;

a filter housing for accommodating a plurality of tunable filters, each of said tunable filters being adapted to receive focused photons from at least one of said plurality of optical lenses to form a filtered spectrum of said sample;

a first optical camera and a second optical camera for receiving the filtered photons from the plurality of tunable filters and forming therefrom a hyperspectral spectrum of the sample;

a controller optically connecting at least one of said plurality of optical lenses with at least one of said plurality of tunable filters.

40. The system of claim 39, wherein said photons from said sample are selected from the group consisting of photons reflected by said sample, photons refracted by said sample, luminescence, fluorescence, Raman scattered photons, photons transmitted by said sample, photons adsorbed by said sample, and photons emitted by said sample.

41. The system of claim 39, wherein said controller optically connects said at least one of said plurality of optical lenses with said at least one of said plurality of tunable filters through a mirror.

42. The system of claim 39, wherein said controller also optically connects said at least one of said plurality of tunable filters with at least one of said first optical camera and said second optical camera.

43. The system of claim 39, further comprising at least one illumination source adapted to illuminate said sample with photons having wavelengths in the range of 250-2500 nm.

44. The system of claim 39, wherein said controller matches one of said plurality of optical lenses with one or more of said plurality of tunable filters.

45. The system of claim 43, wherein said controller defines the wavelength of photons emitted by said illumination source.

46. The system of claim 39, wherein each of said plurality of optical lenses is independently selected from the group consisting of an NIR lens, a UV-VIS lens and a zoom lens.

47. The system of claim 39, wherein at least one of said plurality of optical lenses defines an optical train.

48. The system of claim 39, wherein said plurality of tunable filters includes at least one LCTF.

49. The system of claim 39, wherein each of said plurality of tunable filters is independently selected from the group consisting of a VIS LCTF, a SW-NIR LCTF, a MW-NIR LCTF, a LW-NIR LCTF and a UV-LCTF.

50. The system of claim 39, wherein said hyperspectral image is a spatially accurate wavelength-resolved image of said sample in the wavelength range of 250-2500 nm.

51. The system of claim 39, wherein said system is an integrated device.

52. The system of claim 39, wherein said controller optically connects one of said plurality of optical lenses with one of said plurality of optical tunable filters in order to match the operating wavelength of a predetermined one of said plurality of optical lenses with a predetermined one of said plurality of tunable filters.

53. An automated system for collecting a hyperspectral image of a sample, said automated system including a processor programmed to:

(a) illuminate said sample using an illumination source having a first selected wavelength;

(b) selectively direct one of a plurality of optical lenses to collect photons from said sample and focus said collected photons;

wherein said one of said plurality of optical lenses is adapted to conform to said first selected wavelength;

(c) direct said focused collected photons from said one of said plurality of optical lenses to an optical filter selected from a plurality of optical filters, wherein said selected optical filter is adapted to conform to said first selected wavelength and to receive said focused collected photons and to provide filtered photons;

(d) optically transmit said filtered photons from said selected optical filter to one of a plurality of optical cameras to form an image of said sample at said first selected wavelength;

(e) repeat steps (a)-(d) for a second selected wavelength to obtain a second image of said sample at said second selected wavelength; and

(f) combine said first image of said sample with said second image of said sample to form a hyperspectral image of said sample.

54. The system of claim 53, wherein said photons from said sample are selected from the group consisting of photons reflected by said sample, photons refracted by said sample, luminescence, fluorescence, Raman scattered photons, photons transmitted by said sample, photons adsorbed by said sample, and photons emitted by said sample.

55. The system of claim 53, wherein said hyperspectral image is a spatially accurate wavelength-resolved image of said sample in the wavelength range of 250-2500 nm.

56. The system of claim 53, wherein said first selected wavelength is in the range of 250-700 nm.

57. The system of claim 53, wherein said second selected wavelength is in the range of 700-2100 nm.

58. The system of claim 53, wherein said first selected optical lens is a NIR lens.

59. The system of claim 53, wherein said second selected optical lens is a UV-VIS lens.

60. The system of claim 53, wherein said selected optical filter is selected from the group consisting of a VIS LCTF, a SW-NIR LCTF, a MW-NIR LCTF, a LW-NIR LCTF and a UV-LCTF.

61. The system of claim 53, wherein said optical camera is selected from the group consisting of a CCD and an InGaAs camera.

62. The system of claim 53, wherein said selected optical filter is an LCTF filter.

63. The system of claim 53, wherein the range of said first selected wavelength and the range of said second selected wavelength overlap.

64. The system of claim 53, wherein the range of said first selected wavelength and the range of said second selected wavelength do not overlap.

65. The system of claim 53, wherein said processor is further programmed to electromagnetically record said hyperspectral image of said sample.

66. The system of claim 53, wherein said selected optical filter is a rejection filter.