Label-free high-throughput optical technique for detecting biomolecular interactions

• US 7,422,891 B2
• Filed: 11/12/2004
• Issued: 09/09/2008
• Est. Priority Date: 10/30/2000
• Status: Expired due to Term

• Current Assignee: X-Body Incorporated (Bristol-Myers Squibb Company)
• Original Assignee: Sru Biosystems Incorporated
• Inventors: Cunningham, Brian T.
• Primary Examiner(s): Le; Long V.
• Assistant Examiner(s): YANG, NELSON C
• Application Number:
  US10/988,414
• Publication Number:
  US 20050227374A1
• Time in Patent Office: 1,397 Days
• Field of Search: 356/128, 356/400, 356/401, 356/509, 356/620, 257 82- 84, 359/237, 359/245, 359/333, 359345-347, 359/558, 359/566, 372/6, 372/11, 372/33, 436/518, 436/287.1
• US Class Current: 435/287.2
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/4788 Diffraction for sizing particles G01N15/0205
• CPC Class Codes: G01N 21/7743 the reagent-coated grating coupling light in or out of the waveguide
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G02B 5/1809 with pitch less than or comparable to the wavelength
• CPC Class Codes: Y10T 436/24 Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

Abstract

Methods and compositions are provided for detecting biomolecular interactions. The use of labels is not required and the methods can be performed in a high-throughput manner. The invention also provides optical devices useful as narrow band filters.

Claims

1. A biosensor comprising a two-dimensional grating having a first and a second surface comprised of an optically transparent material that conducts electricity, wherein the first surface of the two-dimensional grating is coated with an electrical insulator, wherein one or more specific binding substances are bound to the electrical insulator, and wherein the second surface of the two-dimensional grating is deposited on a substrate, wherein when the biosensor is illuminated a resonant grating effect is produced on the reflected radiation spectrum, wherein the depth and the period of the two-dimensional grating are less than the wavelength of the resonant grating effect.

2. The biosensor of claim 1, wherein the two-dimensional grating is comprised of a repeating pattern of shapes selected from the group consisting of squares, circles, lines, ellipses, triangles, ovals, trapezoids, sinusoidal waves, rectangles, and hexagons.

3. The biosensor of claim 1, wherein the repeating pattern of shapes are arranged in a rectangular grid or hexagonal grid.

4. The biosensor of claim 1, wherein the two-dimensional grating has a period of about 0.01 microns to about 1 micron and a depth of about 0.01 microns to about 1 micron.

5. The biosensor of claim 1, wherein two or more separate grating regions are present on the same substrate.

6. The biosensor of claim 5, further comprising an electrically conducting trace connected to each separate grating region of the substrate.

7. The biosensor of claim 6, wherein the conducting trace is connected to a voltage source.

8. The biosensor of claim 5, wherein one or more specific binding substances are bound to each separate grating region of the substrate.

9. The biosensor of claim 8, wherein the one or more specific binding substances are bound to their respective binding partners.

10. A liquid-containing vessel comprising the biosensor of claim 1 as an internal surface.

11. The liquid-containing vessel of claim 10, wherein the vessel is selected from the group consisting of a microtiter plate, a test tube, a petri dish and a microfluidic channel.

12. A method of detecting binding of one or more specific binding substances to their respective binding partners comprising:

(a) using the biosensor of claim 6;

(b) applying an electrical charge to the electrically conducting traces;

(c) illuminating the biosensor with light; and

(d) detecting reflected wavelength of light from the biosensor;

wherein, if the one or more specific binding substances have bound to their respective binding partners, then the reflected wavelength of light is shifted.

13. The method of claim 12, further comprising the step of applying a reversed electrical charge to the electrically conducting traces before illuminating the biosensor with light.

14. A detection system comprising the biosensor of claim 1 a light source that directs light at the biosensor, and a detector that detects light reflected from the biosensor, wherein a first illuminating fiber probe having two ends is connected at its first end to the detector, wherein a second collection fiber probe having two ends is connected at its first end to the light source, wherein the first and second fiber probes are connected at their second ends to a third fiber probe, wherein the third fiber probe acts as an illumination and collection fiber probe, and wherein the third fiber probe is oriented at a normal angle of incidence to the biosensor and supports counter-propagating illuminating and reflecting optical signals.

15. A detection system comprising the biosensor of claim 1, a light source that directs light at the biosensor, and a detector that detects light reflected from the biosensor, wherein an illuminating fiber probe is connected to the light source and is oriented at a 90 degree angle to a collecting fiber probe, wherein the collecting fiber probe is connected to the detector, wherein light is directed through the illuminating fiber probe into a beam splitter that directs the light to the biosensor, wherein reflected light is directed into the beam splitter that directs the light into the collecting fiber.

16. A biosensor composition comprising two or more biosensors of claim 1, wherein the biosensors are associated with a holding fixture.

17. The biosensor composition of claim 16, wherein the composition comprises about 50 to about 1,000 individual biosensors.

18. The biosensor composition of claim 16, wherein the two or more biosensors each comprise about 25 to about 1,000 distinct locations.

19. The biosensor composition of claim 16, wherein the holding fixture holds each biosensor such that each biosensor can be placed into a separate well of a microtiter plate.

20. A biosensor composition comprising one or more biosensors of claim 1 on a tip of a multi-fiber optic probe.