System and methods for dynamic range extension using variable length integration time sampling
First Claim
1. A method for processing a photo-detector signal, the method comprising:
- identifying first and second signal components of the photo-detector signal wherein the first and second signal components comprise integrations of the photo-detector signal during first and second time intervals that are temporally proximal to one another and of differing durations;
determining if the first or second signal component exceeds a selected range and generating a scaling value based upon the first and second time intervals for the first or second signal component that does not exceed the selected range;
determining a signal offset value based upon the first and second signal components and the scaling value for the first or second signal component that does not exceed the selected range; and
determining an adjusted signal value by applying the signal offset value and the scaling value to the first or second signal component that does not exceed the selected dynamic range.
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Accused Products
Abstract
A photo-detector generated signal is measured as a sample set comprising a long signal and a short signal. The short signal is scaled to the value of the long signal if the long signal exceeds a dynamic range associated with the photo detector. In one embodiment, the short signal is obtained during a short time interval that is at the approximate middle of a long time interval such that the short and long intervals share a common median time value. Given such symmetry, an approximately linear signal yields a proportionality parameter between the long and short signals thereby allowing the short signal to be scaled. The proportionality parameter facilitates determination of an integration independent component of the photo detector signal that should be removed from the measured long and short signals before scaling. A plurality of sample sets can also be processed such that each sample set overlaps with its neighboring sample set, thereby increasing the effective number of sample sets.
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Citations
51 Claims
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1. A method for processing a photo-detector signal, the method comprising:
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identifying first and second signal components of the photo-detector signal wherein the first and second signal components comprise integrations of the photo-detector signal during first and second time intervals that are temporally proximal to one another and of differing durations; determining if the first or second signal component exceeds a selected range and generating a scaling value based upon the first and second time intervals for the first or second signal component that does not exceed the selected range; determining a signal offset value based upon the first and second signal components and the scaling value for the first or second signal component that does not exceed the selected range; and determining an adjusted signal value by applying the signal offset value and the scaling value to the first or second signal component that does not exceed the selected dynamic range. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method for adjusting a signal generated by a photo-detector signal processor, the method comprising:
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determining a first signal value L and a second signal value S for a sample set wherein the first signal value L corresponds to a signal acquired during a first time interval and wherein the second signal value S corresponds to a signal acquired during a second time interval, wherein the second signal value S is less than the first signal value L and wherein the first signal value L exceeds a specified range; determining a proportionality parameter K between the first and second signal values based upon the first and second time intervals; determining a signal offset parameter C based upon the first and second signal values L and S and the proportionality parameter K; and applying the signal offset parameter C to the second signal value and scaling the resulting offset-applied second signal value by the proportionality parameter K. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31)
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32. A system for processing a photo-detector signal associated with a sequencing apparatus, comprising:
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a photo-detector that detects a labeled sample signal and transforms said labeled sample signal into an electronic signal; a signal processor that acquires one or more sample sets associated with the electronic signal wherein each sample set comprises a first signal value L and a second signal value S wherein the first signal value corresponds to an integrated photo-detector signal acquired during a first time interval and wherein the second signal value corresponds to an integrated photo-detector signal acquired during a second time interval that is less than the first time interval; and wherein the signal processor is configured to determine a proportionality parameter K between the first and second signal values based upon the first and second time intervals, and wherein the signal processor is further configured to determine a signal offset parameter C based upon the first and second signal value and the proportionality parameter, and wherein the signal processor determines a signal value representative of the sample set by applying the signal offset parameter and the proportionality parameter to the second signal value if the first signal value exceeds a specified range. - View Dependent Claims (33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51)
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Specification