System and method

• US 7,425,251 B2
• Filed: 07/20/2001
• Issued: 09/16/2008
• Est. Priority Date: 08/08/2000
• Status: Expired due to Term

• Current Assignee: deltaDOT Limited
• Original Assignee: deltaDOT Limited
• Inventors: Sideris, Dimitrios
• Primary Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US10/344,070
• Publication Number:
  US 20040014231A1
• Time in Patent Office: 2,615 Days
• Field of Search: 204/450, 204/600, 204/603, 204/612, 204/461, 204/452, 422/70, 702 19- 22
• US Class Current: 204/450
• CPC Class Codes: G01N 27/44717 Arrangements for investigating the separated zones, e.g. localising zones
• CPC Class Codes: G01N 27/44721 by optical means
• CPC Class Codes: G01N 27/44791 Microapparatus sample containers with integrated microfluidic structures B01L3/5027

Abstract

A system and method of characterizing, in particular sequencing, polymeric samples, the system comprising: a space-time map generator for generating a space-time map of points representative of the signal peaks of signals detected at a plurality of spaced positions as passed by the migrating components of a plurality of separately-provided sample plugs; and a vertex finder for identifying vertices from the space-time map, with a single vertex being identified for the components of each separately-provided sample plug and the velocities of the sample components being determinable from the points in the space-time map.

Claims

1. A system for characterising, in particular sequencing, polymeric samples, comprising:

a space-time map generator for generating a space-time map of points representative of the signal peaks of signals detected at a plurality of spaced positions as passed by the migrating components of a plurality of separately-provided sample plugs; and

a vertex finder for identifying vertices from the space-time map, with a single vertex being identified for the components of each separately-provided sample plug and the velocities of the sample components being determinable from the points in the space-time map.

2. The system of claim 1, further comprising a velocity sorter for determining the nominal velocities associated with the signal peaks in the signals and grouping those signal peaks into sets according to nominal velocity.

3. The system of claim 1, wherein the space-time map generator is configured to utilize a corrected time component in generating the space-time map according to a function of the electric current variation.

4. The system of claim 1, wherein the space-time map generator is an equiphase space-time map generator for generating an equiphase space-time map of equiphase points.

5. The system of claim 4, wherein the equiphase space-time map generator is configured to transform each data set into a set of local slopes and determine the local extreme as the minimum absolute local derivatives.

6. The system of claim 1, wherein the components are non-labeled.

7. The system of claim 1, wherein the components are labeled.

8. The system of claim 1, wherein the components are migrated through a channel.

9. The system of claim 8, wherein the channel comprises a separation channel through which the components are electrophoretically driven.

10. The system of claim 1, wherein the sample plugs respectively comprise DNA bands having one of the base pair terminations.

11. A method of characterising, in particular sequencing, polymeric samples, comprising the steps of:

generating a space-time map of points representative of the signal peaks of signals detected at a plurality of spaced positions as passed by the migrating components of a plurality of separately-provided sample plugs; and

identifying vertices from the space-time map, with a single vertex being identified for the components of each separately-provided sample plug and the velocities of the sample components being determinable from the points in the space-time map.

12. The method of claim 11, further comprising the steps of determining the nominal velocities associated with the signal peaks in the signals and grouping those signal peaks into sets according to nominal velocity.

13. The method of claim 11, wherein a time component corrected according to a function of the electric current variation is utilized in generating the space-time map.

14. The method of claim 11, wherein the space-time map is an equiphase space-time map of equiphase points.

15. The method of claim 14, wherein the equiphase points are determined by transforming each data set into a set of local slopes of the signals detected at a plurality of spaced positions and determining the local extreme as the minimum absolute local derivatives.

16. The method of claim 11, wherein the components are non-labeled.

17. The method of claim 11, wherein the components are labeled.

18. The method of claim 11, wherein the components are migrated through a channel.

19. The method of claim 18, wherein the channel comprises a separation channel through which the components are electrophoretically driven.

20. The method of claim 11, wherein the sample plugs are provided at spaced time intervals.

21. The method of claim 11, wherein the sample plugs are provided at spaced positions.

22. The method of claim 21, wherein the sample plugs are provided substantially at the same time.

23. The method of claim 11, wherein the sample plugs respectively comprise DNA bands having one of the base pair terminations.

24. An electrophoresis apparatus including a system for characterising, in particular sequencing, polymeric samples, comprising:

a space-time map generator for generating a space-time map of points representative of the signal peaks of signals detected at a plurality of spaced positions as passed by the migrating components of a plurality of separately-provided sample plugs; and

a vertex finder for identifying vertices from the space-time map, with a single vertex being identified for the components of each separately-provided sample plug and the velocities of the sample components being determinable from the points in the space-time map.

25. A system for characterising, in particular sequencing, polymeric samples including a method comprising the steps of:

generating a space-time map of points representative of the signal peaks of signals detected at a plurality of spaced positions as passed by the migrating components of a plurality of separately-provided sample plugs; and

identifying vertices from the space-time map, with a single vertex being identified for the components of each separately-provided sample plug and the velocities of the sample components being determinable from the points in the space-time map.