System and method

• US 7,425,252 B2
• Filed: 07/20/2001
• Issued: 09/16/2008
• Est. Priority Date: 08/08/2000
• Status: Expired due to Term

• Current Assignee: deltaDOT Limited
• Original Assignee: deltaDOT Limited
• Inventors: Sideris, Dimitrios
• Primary Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US10/344,182
• Publication Number:
  US 20040013568A1
• Time in Patent Office: 2,615 Days
• Field of Search: 204/450, 204/600, 204/603, 204/612, 204/461, 204/452, 422/70, 702 19- 22
• US Class Current: 204/450
• CPC Class Codes: G01N 27/44717 Arrangements for investigating the separated zones, e.g. localising zones

Abstract

An analysis system for and method of enabling determination of the velocities of migrating objects, the system comprising an equiphase space-time map generator for generating an equiphase space-time map of equiphase points from each of a plurality of data sets representative of the signals detected at a plurality of spaced positions, with the velocities of the objects being determinable by fitting the sets of equiphase points in the space-time map corresponding to the respective objects.

Claims

1. An analysis system for enabling determination of the velocities of migrating objects, comprising an equiphase space-time map generator for generating an equiphase space-time map of equiphase points from each of a plurality of data sets representative of the signals detected at a plurality of spaced positions, with the velocities of the objects being determinable by fitting the sets of equiphase points in the space-time map corresponding to the respective objects.

2. The system of claim 1, further comprising a velocity sorter for determining the nominal velocities associated with signal peaks in the signals and grouping those signal peaks into sets according to nominal velocity.

3. The system of claim 1, further comprising a vertex finder for identifying at least one vertex from the space-time map, with a single vertex being identified for each group of objects having a common constraint and the velocities of the objects being determinable from the sets of equiphase points in the space-time map as fitted by the respective vertex.

4. The system of claim 1, wherein the space-time map generator is configured to transform each data set into a set of local slopes of the signals detected at a plurality of spaced positions and determine the local extrema as the minimum absolute local derivatives.

5. The system of claim 1, wherein the space-time map generator is configured to utilize a corrected time component in generating the space-time map according to a function of the electric current variation.

6. The system of claim 1, wherein the objects are non-labeled objects.

7. The system of claim 1, wherein the objects are labeled objects.

8. The system of claim 1, wherein the objects are migrated through a channel.

9. The system of claim 8, wherein the channel comprises a separation channel through which the objects are electrophoretically driven.

10. The system of claim 1, wherein the objects comprise components from one sample.

11. The system of claim 1, wherein the objects comprise components from a plurality of separate samples.

12. The system of claim 1, wherein the objects comprise molecular components.

13. The system of claim 1, wherein the objects comprise polymeric components.

14. The system of claim 13, wherein the components comprise DNA bands.

31. A system as claimed in claim 1 wherein the equiphase space-time map generator is configured to determine local extrema of the signals and to generate the space-time map points corresponding to the local extrema.

15. A method of enabling determination of the velocities of migrating objects, comprising the steps of:

determining equiphase points from the signal peaks of each of the signals detected at a plurality of spaced positions; and

generating an equiphase space-time map of the equiphase points, with the velocities of the objects being determinable by fitting the sets of equiphase points in the space-time map corresponding to the respective objects.

16. The method of claim 15, further comprising the steps of determining the nominal velocities associated with the signal peaks in the signals and grouping those signal peaks into sets according to nominal velocity.

17. The method of claim 15, further comprising the step of identifying at least one vertex from the space-time map, with a single vertex being identified for each group of objects having a common constraint and the velocities of the objects being determinable from the sets of equiphase points in the space-time map as fitted by the respective vertex.

18. The method of claim 15, wherein the equiphase points are determined by transforming each data set into a set of local slopes of the signals detected at a plurality of spaced positions and determining the local extrema as the minimum absolute local derivatives.

19. The method of claim 15, wherein a time component corrected according to a function of the electric current variation is utilized in generating the space-time map.

20. The method of claim 15, wherein the objects are non-labeled objects.

21. The method of claim 15, wherein the objects are labeled objects.

22. The method of claim 15, wherein the objects are migrated through a channel.

23. The method of claim 22, wherein the channel comprises a separation channel through which the objects are electrophoretically driven.

24. The method of claim 15, wherein the objects comprise components from one sample.

25. The method of claim 15, wherein the objects comprise components from a plurality of separate samples.

26. The method of claim 15, wherein the objects comprise molecular components.

27. The method of claim 15, wherein the objects comprise polymeric components.

28. The method of claim 27, wherein the components comprise DNA bands.

29. An electrophoresis apparatus including an analysis system for enabling determination of the velocities of migrating objects, comprising an equiphase space-time map generator for generating an equiphase space-time map of equiphase points from each of a plurality of data sets representative of the signals detected at a plurality of spaced positions, with the velocities of the objects being determinable by fitting the sets of equiphase points in the space-time map corresponding to the respective objects.

30. An analysis system for enabling determination of the velocities of migrating objects including a method comprising the steps of:

determining equiphase points from the signal peaks of each of the signals detected at a plurality of spaced positions; and

generating an equiphase space-time map of the equiphase points, with the velocities of the objects being determinable by fitting the sets of equiphase points in the space-time map corresponding to the respective objects.