Apparatus, system, and method of detecting an analyte utilizing pyroelectric technology

• US 7,425,310 B2
• Filed: 01/29/2005
• Issued: 09/16/2008
• Est. Priority Date: 01/29/2004
• Status: Expired due to Fees

• Current Assignee: Nextteq, LLC
• Original Assignee: Bryan Truex, Charles Loomis
• Inventors: Truex, Bryan, Loomis, Charles
• Primary Examiner(s): Le; Long V.
• Assistant Examiner(s): Jung; Unsu
• Application Number:
  US11/045,693
• Publication Number:
  US 20050196322A1
• Time in Patent Office: 1,326 Days
• Field of Search: 436/147
• US Class Current: 422/82.05
• CPC Class Codes: G01N 25/482 concerning the temperature responsive elements measuring temperature or quantity of heat, thermally-sensitive elements G01K; thermoelectric devices H10N10/00, H10N15/00

Abstract

A system for the measurement of a target analyte is provided including a detection apparatus and a reading apparatus. The detection apparatus, or “carrier”, includes a pyroelectric transducer (“pyroelectric film”) and one or more reagent concentrations (“reagent deposit”) deposited on the film. The reagent deposits are adapted to react with, and thus, detect the presence of a target analyte present in the local environment. Upon detection of the target analyte by the reagent deposit, the reading apparatus and the pyroelectric film may be used to detect the amount of heat that can be absorbed by the reagent deposit in response to irradiation. The pyroelectric film then delivers to the reader a signal corresponding to heat detected and the reader provides a corresponding indication of the concentration of the target substance detected.

Claims

1. In a system for measuring a concentration of at least one target analyte, a sensor carrier subsystem comprising:

a transparent substrate having a first surface, a second surface disposed opposite of said first surface, and a reagent deposit supported on said first surface, said reagent deposit being reactive to an exposure to the target analyte and further characterized by a capacity to absorb electromagnetic energy, wherein said absorption capacity changes after said reagent deposit reacts upon the exposure to the target analyte such that said change in capacity is indicative of an amount of the exposure to the target analyte;

an electromagnetic spectrum emitting source (EM source) positioned proximal to said transparent substrate, said EM source being operable to irradiate said reagent deposit by way of a path directed through said transparent substrate; and

a pyroelectric transducer positioned adjacent to said reagent deposit to detect energy absorbed by said reagent deposit upon irradiation by said EM source and generate output signals indicative of the detected energy, such that said output signal is indicative of the exposure to the target analyte; and

wherein the reagent deposit is spaced apart from the pyroelectric transducer.

2. The sensor carrier subsystem of claim 1, wherein said pyroelectric transducer is positioned intermediate said transparent substrate and said EM source, such that said EM source is operable to irradiate said reagent deposit by way of the path being directed through said pyroelectric transducer.

3. The sensor carrier subsystem of claim 1, wherein said transparent substrate is positioned intermediate said transducer and said EM source.

4. The sensor carrier subsystem of claim 1, further comprising a second EM source positioned proximal said reagent deposit such that said first surface is positioned intermediate said second surface and said second EM source.

5. The sensor carrier subsystem of claim 4, wherein said pyroelectnc transducer is positioned intermediate said transparent substrate and said second EM source, such that said second EM source is operable to irradiate said reagent deposit by way of the path being directed through said pyroelectric transducer.

6. The sensor carrier subsystem of claim 5, wherein said pyroelectric transducer is a transparent pyroelectric film.

7. The sensor carrier subsystem of claim 1, wherein said reagent deposit is supported on said transparent substrate such that said reagent deposit faces and is spaced apart from said pyroelectric transducer;

and

wherein said transparent substrate and said pyroelectric transducer are spaced apart so as to provide a sample environment flow path therebetween.

8. The sensor carrier subsystem of claim 1, wherein said transparent substrate further includes a second reagent deposit supported on one of said first and second surfaces.

9. The sensor carrier subsystem of claim 8, wherein said second reagent deposit is disposed on said second surface in substantially vertical alignment with said first reagent deposit supported on said first surface.

10. The sensor carrier subsystem of claim 8, further comprising a second EM source positioned relative to said first EM source, such that said transparent substrate, said first and second reagent deposits, and said pyroelectric transducer are positioned intermediate said first and second EM sources.

11. The sensor carrier subsystem of claim 8, further comprising a second EM source positioned proximal said first EM source, such that said pyroelectric transducer is positioned intermediate said transparent substrate and said first and second EM sources.

12. The sensor carrier subsystem of claim 1, further comprising a second substrate supporting a reagent deposit on a first surface of said second substrate and spaced apart from said pyroelectric transducer such that said second reagent deposit faces said pyroelectric transducer, wherein said EM source is operable to irradiate said second reagent deposit by way of a path directed through said transparent substrate and through said pyroelectric transducer.

13. The sensor carrier subsystem of claim 1, further comprising:

a second transparent substrate having a first surface and a second surface disposed opposite of said first surface, wherein a second reagent deposit is supported on said first surface and spaced apart from said pyroelectric transducer such that said second reagent deposit faces said pyroelectric transducer; and

a second EM source positioned proximal said second surface of said second transparent substrate and operable to irradiate said second reagent deposit by way of a path directed through said first and second surfaces of said second transparent substrate.

14. The sensor carrier subsystem of claim 1, further comprising a reflective surface positioned to redirect energy emitted by said EM source through said reagent deposit, said reflective surface being positioned relative to said EM source such that said transparent substrate, said reagent deposit, and said pyroelectric transducer are positioned intermediate said reflective surface and said EM source.

15. The sensor carrier subsystem of claim 14, wherein said reflective surface is a mirror spaced directly across from said reagent deposit.

16. The sensor carrier subsystem of claim 1, wherein said pyroelectnc transducer includes a pyroelectnc film having conductive electrodes thereon for transmitting an output signal.

17. The sensor carrier subsystem of claim 16, wherein said pyroelectric film is substantially transparent to said energy irradiated from said EM source.

18. The sensor carrier subsystem of claim 17, wherein said EM source is selected from a group consisting of visible light emitting sources, UV light sources, IR light emitting sources and microwave sources.

19. The sensor carrier subsystem of claim 1 wherein said reagent deposit is affecting a reflected EM spectrum upon chemical interaction with the analyte by one or two means consisting from shifting the reflected EM spectrum and changing the intensity of the reflected EM spectrum.

20. The sensor carrier subsystem of claim 19, wherein said EM source is a light emitting source and said pyroelectric transducer is adapted to detect the amount of heat absorbed in said reagent deposit upon illumination by said light emitting source.

21. The sensor carrier subsystem of claim 1, further comprising a fiber optic cable operatively or light bar associated with said EM source to direct EM energy emitted from the EM source via said path to said reagent deposit.

22. The sensor carrier subsystem of claim 1, wherein said reagent deposit is affecting a reflected EM spectrum upon biological interaction with the analyte by one or two means consisting from shifting the reflected EM spectrum and changing the intensity of the reflected EM spectrum.

23. The sensor carrier subsystem of claim 1, wherein said reagent deposit is affecting a reflected EM spectrum upon physical interaction with the analyte by one or two means consisting from shifting the reflected EM spectrum and changing the intensity of the reflected EM spectrum.

24. The sensor carrier subsystem of claim 1, further comprising an immunoassay sensor for detecting a presence of a particular analyte comprising:

a solid substrate, which changes pyroelectric properties;

an antibody, to which the analyte binds, immobilized on the solid substrate;

a transducer surface providing at least one electrophoretic separation layer;

an electrode means that provides a voltage signal indicative of heat generated on said transducer surface;

a means of irradiation of the transducer surface by the EM source whereby heat is generated; and

,

a tagged form of the analyte displaceably bound to the antibody and having lower binding energy to the antibody than untagged analyte, wherein untagged analyte will displace tagged analyte and bind to the antibody when the antibody with tagged analyte contacts a sample containing untagged analyte, the antibody with bound tagged analyte having pyroelectric property, which is changed by displacement of tagged analyte by untagged analyte, the antibody with bound tagged analyte forming a solid state system.

25. The immunoassay sensor of claim 24, further comprising a pyroelectric detector operatively associated with the solid substrate to detect changes in pyroelectric property produced by displacement of the tagged analyte by untagged analyte.

26. The immunoassay sensor of claim 24, wherein the solid substrate is a membrane.

27. The immunoassay sensor of claim 24, wherein the transducer surface is transparent at a light frequency so as to irradiate said antibody, to which the analyte binds, immobilized on the solid substrate.

28. The immunoassay sensor of claim 24, wherein the antibody is one or more selected from a group consisting of a monoclonal antibody, a pool of monoclonal antibodies, and a polyclonal antibody.

29. The sensor carrier subsystem of claim 1 further comprising an immunoassay sensor based on immunochromatograpy techniques for detecting the presence of a particular analyte comprising:

a conjugate antibody release pad that contains at least one pre-selected conjugated antibody to bind to the target analyte to create a conjugated antibody bound analyte;

a sample delivery pad to filter out any undesired matter and to hold the conjugated antibody bound analyte so that said conjugated antibody bound analyte can slowly wick to the antibody conjugate release pad;

an analysis pad to hold the conjugated antibody bound analyte;

a transducer surface providing at least one electrophoretic separation layer adjacent to the analysis pad;

an electrode means that provides a voltage signal indicative of heat generated on said transducer surface;

a means of irradiation of the transducer surface with light frequency whereby heat is generated;

a buffer to control a pH level of the sample;

a surfactant to prevent formation of an aggregate; and

,

a means of separating said conjugate antibody release pad from said analysis pad after a bound conjugated antibody flows to the analysis pad to prevent the sample from flowing back to the conjugate antibody release pad.

30. The sensor carrier subassembly of claim 29, further comprising a pyroelectric detector operatively associated with the analysis pad to detect changes in pyroelectric property produced by displacement of a tagged analyte by an untagged analyte.

31. The immunoassay sensor of claim 29, wherein the transducer surface is transparent at said light frequency so as to irradiate said conjugated antibody to which the target analyte binds.

32. The immunoassay sensor of claim 29, wherein the at least one pre-selected conjugated antibody is one or more selected from a group consisting of a monoclonal antibody, a pool of monoclonal antibodies, and a polyclonal antibody.

33. The systems of claim 1, wherein the EM source generates energy in a microwave frequency spectrum.

34. The systems of claim 1, wherein the reader contains a timer.

35. The systems of claim 1, wherein the reagent is reversible and reusable.

36. The systems of claim 1, wherein the reagent is non-reversible and reusable.

37. The systems of claim 1, wherein the reagent is an inkjet printing.

38. The systems of claim 1, wherein the reagent is a screen.

39. The systems of claim 1, wherein the reagent is cast in a mold onto the substrate such that the molded reagent is symmetrical on the top, bottom and sides.

40. The systems of claim 1, wherein the EM source is located on a removable assembly subcomponent of the system.

41. The systems of claim 1, wherein a filter assembly is located on a removable assembly subcomponent of the system.

42. The systems of claim 1, wherein said transparent substrate contains a memory chip.

43. A system for detecting a presence of at least one target analyte in a sample local environment, said system comprising:

a transparent substrate having a first surface, a second surface disposed opposite of said first surface, and a reagent deposit supported on said first surface, said reagent deposit being reactive to an exposure to the target analyte and further characterized by a capacity to absorb electromagnetic energy, wherein said absorption capacity changes after said reagent deposit reacts upon the exposure such that said change in capacity is indicative of the exposure to the target analyte;

an electromagnetic spectrum emitting source (EM source) positioned proximal to said transparent substrate and closer to the second surface than to the first surface, said EM source being operable to irradiate said reagent deposit by way of a path directed through said transparent substrate;

a pyroelectric transducer positioned proximal to said reagent deposit to detect energy absorbed by said reagent deposit upon irradiation by said EM source and generate output signals indicative of the detected energy, such that said output signal is indicative of the exposure to the target analyte;

a reader operatively associated with said pyroelectric transducer to receive said output signals, and communicate, therefrom, an indication of the presence of the target analyte in the sample local environment, and

wherein the reagent deposit is spaced apart from the pyroelectric transducer.

44. The system of claim 43, wherein said pyroelectric transducer is positioned intermediate said transparent substrate and said EM source, such that said EM source is operable to irradiate said reagent deposit by way of the path being directed through said pyroelectric transducer.

45. The carrier subsystem of claim 43, wherein said transparent substrate is positioned intermediate said transducer and said EM source.

46. The system of claim 43, further comprising a second EM source positioned proximal said reagent deposit such that said first surface is positioned intermediate said second surface and said second EM source.

47. The system of claim 46, wherein said pyroelectric transducer includes a transparent pyroelectric film positioned intermediate said transparent substrate and said second EM source, such that said second EM source is operable to irradiate said reagent deposit by way of a path directed through said pyroelectric transducer.

48. The system of claim 43, wherein said reagent deposit is colorimetrically reactive to a target analyte and said EM source is a light emitting diode.

49. The system of claim 43, wherein said reagent deposit is supported on said transparent substrate such that said reagent deposit faces and is spaced apart from said pyroelectric transducer and wherein said transparent substrate and said pyroelectric transducer are spaced apart so as to provide a sample environment flow path therebetween.

50. The system of claim 43, wherein said transparent substrate further includes a second reagent deposit supported on said second surface and disposed on said second surface in substantially vertical alignment with said first reagent deposit supported on said first surface.

51. The subsystem of claim 43, further comprising a second substrate supporting a reagent deposit on a first surface of said second substrate and spaced apart from said pyroelectric transducer such that said second reagent deposit faces said pyroelectric transducer, wherein said EM source is operable to irradiate said second reagent deposit by way of the path being directed through said pyroelectric transducer.

52. The systems of claim 43, wherein the EM source generates energy in a microwave frequency spectrum.

53. The systems of claim 43, wherein the reader contains a timer.

54. The systems of claim 43, wherein the reagent is reversible and reusable.

55. The systems of claim 43, wherein the reagent is non-reversible and reusable.

56. The systems of claim 43, wherein the reagent is an inkjet.

57. The systems of claim 43, wherein the reagent is a screen printing.

58. The systems of claim 43, wherein the reagent is cast in a mold onto the substrate such that the molded reagent is symmetrical on the top, bottom and sides of the substrate.

59. The systems of claim 43, wherein the EM source is located on a removable assembly subcomponent of the system.

60. The systems of claim 43, wherein the fitter assembly is located on a removable assembly subcomponent of the system.

61. The systems of claim 43, wherein said transparent substrate contains a memory chip.