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Method for detecting methylated CpG islands

  • US 7,425,415 B2
  • Filed: 04/06/2006
  • Issued: 09/16/2008
  • Est. Priority Date: 04/06/2005
  • Status: Active Grant
First Claim
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1. A method of detecting methylated CpG islands which comprises:

  • (a) purifying genomic DNA from a sample;

    (b) incubating a methylated CpG island binding protein, a binding partner for the binding protein, and the purified genomic DNA to produce bound DNA, wherein the bound DNA comprises genomic DNA having methylated CpG islands bound to the said binding protein, having bound the binding partner;

    (c) isolating the bound DNA; and

    (d) amplifying the bound DNA to detect methylated CpG islands.

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