Bioreactor for culturing microorganisms

• US 7,425,441 B2
• Filed: 04/11/2005
• Issued: 09/16/2008
• Est. Priority Date: 04/16/2004
• Status: Expired due to Fees

• Current Assignee: Sartorius Stedim Biotech GmbH (Sartorius AG)
• Original Assignee: Sartorius Stedim Biotech GmbH (Sartorius AG)
• Inventors: Salzmann, Rainer, Wilhelm, Bernd-Ulrich, Franke, Horst, Broneske, Jürgen, Keitel, Heinz-Rüdiger, Kröger, Wolfgang, Riese, Mirko, Linke, Wolf-Dietrich, Pulz, Otto
• Primary Examiner(s): Beisner; William H.
• Assistant Examiner(s): HOBBS, MICHAEL L
• Application Number:
  US11/103,148
• Publication Number:
  US 20050255584A1
• Time in Patent Office: 1,254 Days
• Field of Search: 435/293.1, 435/295.2, 435/257.1, 435/261, 435/420
• US Class Current: 435/292.1
• CPC Class Codes: C12M 21/02 Photobioreactors culturing algae A01G33/00, A01H4/001, C12N1/12
• CPC Class Codes: C12M 23/44 Multiple separable units; Modules
• CPC Class Codes: C12M 29/18 External loop; Means for reintroduction of fermented biomass or liquid percolate loop type reactors for chemical or physical processes B01J19/2435
• CPC Class Codes: C12M 29/22 Oxygen discharge
• CPC Class Codes: C12M 31/02 located outside the reactor
• CPC Class Codes: C12M 41/12 of temperature controlling the temperature of chemical or physical processes B01J19/0013, heating or cooling apparatus for laboratory use B01L7/00
• CPC Class Codes: C12M 41/18 Heat exchange systems, e.g. heat jackets or outer envelopes
• CPC Class Codes: C12M 41/26 of pH
• CPC Class Codes: C12M 41/34 of gas
• CPC Class Codes: C12M 41/36 of biomass, e.g. colony counters or by turbidity measurements electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404

Abstract

A bioreactor for culturing microorganisms which has a reactor vessel, a plurality of gas-introduction tubes and a gas-introduction system for introducing gas into a culture medium in the gas-introduction tubes via injectors, wherein the gas-introduction tubes are connected by their respective lower end, in the vertical direction, to the reactor vessel and by their opposite upper end to the upper end of an upright vessel which is likewise connected by its lower end to the reactor vessel, and at the upper end of the upright vessel an expansion vessel is arranged.

Claims

1. A bioreactor (1) for culturing microorganisms comprising a reactor vessel (2) which is constructed as a heat exchanger, a plurality of gas-introduction tubes (3) and a gas-introduction system (6) for introducing gas into a culture medium in the gas-introduction tubes (3) via injectors (26), wherein the gas-introduction tubes (3) are connected by their respective lower end (17), in the vertical direction, to the reactor vessel (2) and by their opposite upper end (19) to an upper end (16) of an upright vessel (4) which is likewise connected by its lower end (14) to the reactor vessel (2), and at the upper end (14) of the upright vessel (4) an expansion vessel (5) is arranged, said reactor vessel (2) having a reactor lower part (7) having a biomass chamber (12), and the reactor lower part (7) is covered by an attachment lid (8) which has attachment flanges (18) for the gas-introduction tubes (3), attachment flanges (45) for sensors (28) and an attachment flange (13) for the upright vessel (4).

2. The bioreactor as claimed in claim 1, wherein the gas-introduction tubes (3) are constructed so as to be at least in part transparent for the culture phototrophic microorganisms.

3. The bioreactor as claimed in claim 1, wherein the upright vessel (4) is constructed as a standpipe (15) having a circular cross section.

4. The bioreactor as claimed in claim 3, wherein the upright vessel (4) has an open cross-sectional area which approximately corresponds to the total of all open cross-sectional areas of the gas-introduction tubes (3).

5. The bioreactor as claimed in claim 4, wherein the expansion vessel (5) has a lid (30) on which are disposed a feed device (36) and an exhaust gas apparatus (33) having exhaust-gas cooler (34) and exhaust-gas filter (35).

6. The bioreactor as claimed in claim 1, wherein the gas-introduction tubes (3) are connected via the expansion vessel (5) to the upright vessel (4).

7. The bioreactor as claimed in claim 6, wherein the gas-introduction tubes are connected via tube bends (20) to the upright vessel (4).

8. The bioreactor as claimed in claim 7, wherein the gas-introduction tubes (3), the tube bends (20), the expansion vessel (5) and the upright vessel (4) are constructed of glass.

9. The bioreactor as claimed in claim 1, wherein the reactor lower part (7) has an outwardly dished base (9).

10. The bioreactor as claimed in claim 9, wherein the reactor lower part (7) has, in the biomass chamber (12), an inwardly dished intermediate base (10) which, together with the outwardly dished base (9), forms a heating or cooling chamber (11).

11. The bioreactor as claimed in claim 10, wherein the heating or cooling chamber (11) can be heated or cooled via a heating or cooling device (27) by feeding a heating or cooling medium, and, via the heating or cooling chamber (11), heat can be exchanged between the culture medium in the biomass chamber (12) and the heating or cooling medium in the heating or cooling chamber (11).

12. The bioreactor as claimed in claim 11, wherein the heating or cooling device (27) consists of a closed hot water pressure system having a circulation pump and a heat exchanger for cooling water, and the temperature of the culture medium can be used as control parameter.

13. The bioreactor as claimed in claim 12, wherein a plurality of light sources (38) of an illumination device (37) are arranged adjacently to the gas-introduction tubes (3) and the upright vessel (4).

14. The bioreactor as claimed in claim 13, wherein the upright vessel (4) has at least one vertical hollow chamber in which at least one light source (38) is arranged.

15. The bioreactor as claimed in claim 14, wherein the vertical hollow chamber is formed by a tube arranged in the upright vessel (4).

16. The bioreactor as claimed in claim 14, wherein the light sources (38) are constructed as fluorescent tubes (39) which are arranged in parallel to the gas-introduction tubes (3) and to the upright vessel (4).

17. The bioreactor as claimed in claim 16, wherein the illumination device (37) can be dimmed.

18. The bioreactor as claimed in claim 16, wherein a digital measurement and control system (29) is provided for monitoring and open-loop control of the culture process.

19. The bioreactor as claimed in claim 18, wherein the digital measurement and control system (29) is suitable for the measurement and display of temperature, pH, pO₂ and optical density.

20. The bioreactor as claimed in claim 19, wherein the standpipe (4) is constructed centrally to the reactor vessel (2) which is constructed so as to be circular transversely to the vertical and the gas-introduction tubes (3) are arranged in a circle shape around the standpipe (4).

21. The bioreactor as claimed in claim 19, wherein the upright vessel (4) and at least a part of the gas-introduction tubes (3) are arranged in at least one vertical plane.

22. The bioreactor as claimed in claim 21, wherein the reactor vessel (2) transforms at least in part into a horizontal lower connection tube to which the lower ends of the gas-introduction tubes are flanged, and an upper connection tube is provided to which the upper ends (19) of the gas-introduction tubes (3) are connected to the upper end (16) of the upright vessel (4), or to the expansion vessel (5) arranged at the upper end (16) of the upright vessel (4).