Slide photograph data creation system, and slide photograph data

• US 7,426,345 B2
• Filed: 10/04/2005
• Issued: 09/16/2008
• Est. Priority Date: 04/11/2005
• Status: Expired due to Fees

• Current Assignee: Claro Incorporated
• Original Assignee: Claro Incorporated
• Inventors: Sato, Tatsusuke, Miura, Hiromi, Zhongxi, Zheng, Takamatsu, Terumasa, Yasuike, Hidekatsu, Nozaka, Hiroyuki, Miura, Tomisato
• Primary Examiner(s): Perkey; W. B.
• Assistant Examiner(s): WILLIAMS, ANDREW P
• Application Number:
  US11/241,942
• Publication Number:
  US 20060228107A1
• Time in Patent Office: 1,078 Days
• Field of Search: 348/42, 348/44, 348/51, 348/53, 348/79, 348/231.2, 348/231.3, 348/333.05, 396/432, 359/363, 359368-398, 211/41.1, 211/41.8, 211/41.14, 414/331.14, 345/629, 356/609, 382/154
• US Class Current: 396/432
• CPC Class Codes: G01N 1/312 for samples mounted on planar substrates
• CPC Class Codes: G02B 21/367 providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison

Abstract

A slide photograph data creation system includes a digital camera for making high-magnification photographs of samples, a sample transporter for holding and transporting samples, a controller for controlling the camera and the sample transporter so as to provide overlapping photographs, a pasting information generator for recognizing the margin by which the photographs overlap and for generating pasting-together information, and a photograph file generator for storing in a single file a plurality of high-magnification photographs and the pasting-together information. The system facilitates the examination and photography of large numbers of samples, and enables discrimination of the three-dimensional structure of a sample. Since the photographic data is managed with a database, virtual slide photographs and their attributes information can be browsed over a network or the Internet.

Claims

1. A slide photograph data creation system, comprising:

digital photography means provided in a microscope, for photographing high-magnification photographs of samples;

sample transport means for holding and transporting said samples three-dimensionally;

photography control means for controlling said digital photography means to photograph a plurality of said high-magnification photographs with a predetermined interval, and for controlling said sample transport means to successively move said samples perpendicularly to an optical axis of said microscope to provide a margin where one of said photographs partly overlaps with another of said photographs, said sample transport means also moving the sample minutely along the optical axis of said microscope in each of locations where the sample is moved perpendicular to the optical axis, to thereby obtain the three-dimensional high-magnification photographs;

pasting information generation means for recognizing said margin by photograph processing from said plurality of high-magnification photographs, and for generating pasting information of said plurality of high-magnification photographs;

external digital camera means for photographing an entire image of said samples, different from the digital photography means, and

photograph file generation means for storing in one file said entire image of said samples, said plurality of high-magnification photographs and said pasting-together information.

2. The slide photograph data creation system according to claim 1, further comprising focal position detection means for detecting a position where focus of said microscope is matched in a direction parallel to the optical axis of said microscope.

3. The slide photograph data creation system according to claim 1, further comprising thumbnail photograph generation means for generating reduced-size photographs of an entire photograph by pasting together said high-magnification photographs.

4. The slide photograph data creation system according to claim 1, wherein said photographs made by said external digital camera means, and said plurality of high-magnification photographs, and said pasting information are stored by being combined in a single motion picture file.

5. The slide photograph data creation system according to claim 1, further comprising photographic area automatic recognition means for automatically determining, from the image made by said external digital camera means, an area to photograph said high-magnification photograph.

6. The slide photograph data creation system according to claim 5, further comprising photographic area semi-automatic recognition means for list-displaying said external digital camera photograph for plural preparates together with a photographic area recognized by said photographic area automatic recognition means, to correct said photographic area.

7. The slide photograph data creation system according to claim 1, further comprising photograph publishing means for registering files of said photographs in a database, and for enabling searching and referencing of said photographs over a network.

8. The slide photograph data creation system according to claim 1, wherein said sample transport means comprises:

a first guide member in a fixed position on a stand;

a first movable body, supported to be guided on said first guide member, to move horizontally in a front-back direction of said stand;

a second guide member on said first movable body;

a second movable body, supported to be guided on said second guide member, to move horizontally in a left-right direction of said stand;

a preparate holding hand provided on said second movable body to ascend and descend, said preparate holding hand comprising a pair of finger parts for respectively supporting two ends of a preparate horizontally from beneath, and a suction hole, provided on an upper side of at least one of said finger parts, communicating with a negative pressure source for suction-holding said preparate;

a third guide member in a fixed position on said stand; and

a third movable body, supported to be guided on said third guide member to ascend and descend on said stand.

9. The slide photograph data creation system according to claim 8, further comprising a preparate storage magazine, supported on said third movable body and comprising a pair of side wall plates having plural preparate holding slots arranged in parallel vertically for holding each end of said preparate to receive plural preparates arranged vertically in a shelf-like manner at a vertical spacing such that said finger parts can be inserted and removed.

10. The slide photograph data creation system according to claim 9, wherein said preparate storage magazine is attached to and detached from said third movable body.

11. The slide photograph data creation system according to claim 9, wherein said storage magazine comprises:

an attachment/detachment lever to be manually switched for displacement between a locked position for fixing said magazine to said third movable body, and an unlocked position for releasing said magazine from said third movable body; and

a movable stopper operationally linked to said displacement of the attachment/detachment lever for preventing release of said preparates from the magazine when said attachment/detachment lever is in the unlocked position, and for enabling insertion and removal of said preparates when said attachment/detachment lever is in the locked position.