Nanoparticles for optical sensors

• US 7,427,491 B2
• Filed: 07/09/2004
• Issued: 09/23/2008
• Est. Priority Date: 07/10/2003
• Status: Expired due to Fees

• Current Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Original Assignee: Roche Diagnostics Operations Incorporated (Roche Holding AG)
• Inventors: Desprez, Valerie, Spinke, Jurgen, Oranth, Norbert, Tusa, James Kenneth
• Primary Examiner(s): GITOMER, RALPH J
• Application Number:
  US10/888,392
• Publication Number:
  US 20050019849A1
• Time in Patent Office: 1,537 Days
• Field of Search: 435/14, 204/403.04, 436/518, 436/528
• US Class Current: 435/14
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: G01N 33/521 Single-layer analytical elements

Abstract

The present invention concerns a polymer layer for a sensor, wherein the polymer layer has nanoparticles embedded therein which impart to the polymer layer recognizing properties as well as transducer properties, a sensor comprising such layer and the use of the sensor for detecting and/or quantifying a target analyte.

Claims

1. A sensor comprising a polymer layer, said polymer layer comprising recognizing and transducing properties, wherein

said recognizing properties are provided by a recognizing component,

said transducing properties are provided by a transducer, and

said recognizing component and said transducer are contained in one or more solid or semi-solid nanoparticles, wherein said nanoparticles have a mean diameter of between about 10 and 500 nm and wherein said nanoparticles are embedded in said polymer layer.

2. The sensor of claim 1, wherein said nanoparticles further comprise first or second nanoparticles, and wherein

said first nanoparticles comprise said recognizing component, and

said second nanoparticles comprise said transducer.

3. The sensor of claim 1, wherein said nanoparticles embedded in said polymer layer comprise both recognizing component and said transducer.

4. The sensor of claim 1, wherein said recognizing component comprises a bioactive component.

5. The sensor of claim 4, wherein said bioactive component comprises an enzyme.

6. The sensor of claim 5, wherein said enzyme is selected from the group consisting of synthetic enzymes and gene-manipulated enzymes.

7. The sensor of claim 5, wherein said enzyme comprises an oxidative enzyme.

8. The sensor of claim 7, wherein said oxidative enzyme is selected from the group consisting of glucose oxidase, cholesterol oxidase, lactate oxidase, sarcosine oxidase, and mixtures thereof.

9. The sensor of claim 7, wherein the oxidase enzyme comprises glucose oxidase.

10. The sensor of claim 1, wherein said transducer comprises an optical transducer.

11. The sensor of claim 10, wherein said optical transducer comprises a luminescent dye.

12. The sensor of claim 11, wherein said luminescent dye is selected from the group consisting of [Ru(diphenylphenantroline)₃], octaethyl-Pt-porphyrin, octaethyl-Pt-porphyrin ketone, tetrabenzo-Pt-porphyrin, tetraphenyl-Pt-porphyrin, meso-tetraphenyl-tetrabenzo-Pt-porphyrin, tetracylcohexenyl-Pt-porphyrin, octaethyl-Pt-chlorine, tetraphenyl-Pt-chlorine, and mixtures thereof.

13. The sensor of claim 1, wherein said nanoparticles comprise a polymer material.

14. The sensor of claim 13, wherein said polymer material is selected from the group consisting of polyolefins, vinyl polymers, polyamides, polyesters, polyacetals, polycarbonates, polyurethanes, polysiloxanes, copolymers, and mixtures thereof.

15. The sensor of claim 1, wherein said polymer layers comprises a polymer selected from the group consisting of polyolefins, vinyl polymers, polyamides, polyesters, polyacetals, polycarbonates, polyurethanes, polysiloxanes, copolymers, and mixtures thereof.

16. The sensor of claim 1, wherein said polymer layers comprises one or more polyurethanes.

17. The sensor of claim 1, wherein said recognizing component and said transducer are entrapped within, conjugated to, or attached to one or more said nanoparticles embedded in said polymer layer.

18. The sensor of claim 1, wherein said sensor is used for detecting and/or quantifying a target analyte.

19. The sensor of claim 18, wherein said target analyte is selected from the group consisting of glucose, lactate, sarcosine, creatine, and mixtures thereof.

20. The sensor of claim 18, wherein said target analyte is glucose.

21. The sensor of claim 18, wherein said target analyte is contained within a body liquid sample.

22. A sensor comprising:

a light-transmissive substrate;

a polymer layer comprising recognizing and optical transducing properties wherein

said recognizing properties are provided by a recognizing component,

said optical transducing properties are provided by an optical transducer,

said recognizing component and said optical transducer are contained in one or more solid or semi-solid nanoparticles, wherein said nanoparticles have a mean diameter of between 10 and about 500 nm,

said nanoparticles are embedded in said polymer layer, and

said polymer layer is deposited on said light-transmissive substrate, and

a membrane layer positioned on top of said polymer layer.