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Planar patch-clamp cartridge with integrated electrode

  • US 7,429,316 B1
  • Filed: 11/03/2003
  • Issued: 09/30/2008
  • Est. Priority Date: 10/09/2001
  • Status: Expired due to Fees
First Claim
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1. A patch-clamp cartridge comprising:

  • an extracellular component including a plurality of separate extracellular chambers formed in an extracellular structure of unitary construction, each extracellular chamber being isolated from other extracellular chambers and sufficiently deep to surround a respective portion of extracellular fluid required for patch-clamp testing;

    an intracellular component including a plurality of separate intracellular chambers formed in an intracellular structure of unitary construction, each intracellular chamber being isolated from other intracellular chambers and corresponding to a respective extracellular chamber;

    an apertured partition sandwiched between the extracellular component and the intracellular component;

    a common extracellular electrode connecting said plurality of extracellular chambers in said extracellular component through a corresponding plurality of extracellular electrode elements exposed to said extracellular chambers through an interface between the partition and the extracellular component; and

    a plurality of individual intracellular electrodes, each of said intracellular electrodes being inserted in a respective intracellular chamber.

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