Planar patch-clamp cartridge with integrated electrode

• US 7,429,316 B1
• Filed: 11/03/2003
• Issued: 09/30/2008
• Est. Priority Date: 10/09/2001
• Status: Expired due to Fees

• Current Assignee: Molecular Devices
• Original Assignee: MDS Analytical Technologies US Incorporated (Danaher Corporation)
• Inventors: Osipchuk, Yuri
• Primary Examiner(s): Olsen; Kaj K
• Application Number:
  US10/700,652
• Time in Patent Office: 1,793 Days
• Field of Search: 204/403.01, 435/287.1, 435/288.4
• US Class Current: 204/403.01
• CPC Class Codes: G01N 33/48728 Investigating individual cells, e.g. by patch clamp, voltage clamp investigating individual particles in general G01N15/10

Abstract

A planar patch-clamp cartridge includes a common ground electrode through a conductive medium reaching into each extracellular chamber. The cartridge is first primed with intracellular solution injected from dispensing tips inserted from above into the intracellular chambers. Then the cartridge is rotated 180 degrees, whereby the intracellular and extracellular chambers assume face-down and face-up positions, respectively. Extracellular solution is then delivered using the same dispensing unit. A plurality of silver tubes engages the intracellular chambers at the end of the rotation to provide electrical conductivity and suction to the intracellular chambers.

Claims

1. A patch-clamp cartridge comprising:

an extracellular component including a plurality of separate extracellular chambers formed in an extracellular structure of unitary construction, each extracellular chamber being isolated from other extracellular chambers and sufficiently deep to surround a respective portion of extracellular fluid required for patch-clamp testing;

an intracellular component including a plurality of separate intracellular chambers formed in an intracellular structure of unitary construction, each intracellular chamber being isolated from other intracellular chambers and corresponding to a respective extracellular chamber;

an apertured partition sandwiched between the extracellular component and the intracellular component;

a common extracellular electrode connecting said plurality of extracellular chambers in said extracellular component through a corresponding plurality of extracellular electrode elements exposed to said extracellular chambers through an interface between the partition and the extracellular component; and

a plurality of individual intracellular electrodes, each of said intracellular electrodes being inserted in a respective intracellular chamber.

2. The cartridge of claim 1, wherein said extracellular electrode includes a metal coating deposited at said interface.

3. The cartridge of claim 2, wherein said metal is silver.

4. The cartridge of claim 3, further including a silver-chloride layer deposited over said silver coating.

5. The cartridge of claim 1, wherein said extracellular electrode includes a metal wire placed at said interface.

6. The cartridge of claim 5, wherein said metal is silver.

7. The cartridge of claim 6, further including a silver-chloride layer deposited over said silver wire.

8. The cartridge of claim 6, further including a semipermeable layer deposited over said silver wire.

9. The cartridge of claim 1, wherein said extracellular electrode includes a conductive gel placed at said interface.

10. A patch-clamp cartridge comprising:

an apertured partition sandwiched between an extracellular component and an intracellular component; and

an electrode connecting a plurality of extracellular chambers in said extracellular component through a corresponding plurality of electrode elements exposed to said chambers through an interface between the partition and the extracellular component;

wherein said electrode includes a silver wire placed at said interface and a gold layer deposited over said silver wire.

11. The cartridge of claim 10, further including a semipermeable layer deposited over said silver wire.

12. The cartridge of claim 10, wherein said extracellular electrode includes a conductive gel placed at said interface.