Apparatus, system, and method of detecting an analyte utilizing pyroelectric technology

• US 7,429,357 B2
• Filed: 04/12/2006
• Issued: 09/30/2008
• Est. Priority Date: 01/29/2004
• Status: Expired due to Fees

• Current Assignee: Nextteq, LLC
• Original Assignee: Bryan Truex, Charles Loomis
• Inventors: Truex, Bryan, Loomis, Charles
• Primary Examiner(s): Le; Long V.
• Assistant Examiner(s): Jung; Unsu
• Application Number:
  US11/403,218
• Publication Number:
  US 20070048788A1
• Time in Patent Office: 902 Days
• Field of Search: 436/147, 422/82.01, 422/82.02, 422/82.05, 422/82.12
• US Class Current: 422/82.05
• CPC Class Codes: G01N 25/482 concerning the temperature responsive elements measuring temperature or quantity of heat, thermally-sensitive elements G01K; thermoelectric devices H10N10/00, H10N15/00

Abstract

A system for the measurement of a target analyte is provided including a detection apparatus and a reading apparatus. The detection apparatus, or “carrier”, includes a pyroelectric transducer (“pyroelectric film”) and one or more reagent concentrations (“reagent deposit”) deposited on the film. The reagent deposits are adapted to react with, and thus, detect the presence of a target analyte present in the local environment. Upon detection of the target analyte by the reagent deposit, the reading apparatus and the pyroelectric film may be used to detect the amount of heat that can be absorbed by the reagent deposit in response to irradiation. The pyroelectric film then delivers to the reader a signal corresponding to heat detected and the reader provides a corresponding indication of the concentration of the target substance detected.

Claims

1. A method of detecting the presence of a target analyte in a sample environment utilizing a reagent deposit reactive to exposure to the target analyte and said reagent deposit further characterized by a capacity to absorb electromagnetic energy irradiated thereupon, wherein an absorption capacity of the reagent deposit changes after exposure to the target analyte, said method comprising the steps of:

providing a transparent substrate having a first surface, a second surface disposed opposite the first surface, and a reagent deposit supported on one of either the first or second surfaces;

positioning an electromagnetic energy emitting source (EM source) relative to the transparent substrate such that the second surface is positioned intermediate the first surface and the EM source;

positioning a pyroelectric transducer for detecting energy absorbed by the reagent deposit adjacent the reagent deposit;

directing a sample environment past the reagent deposit, thereby initiating a reaction between the reagent deposit and any target analyte in the sample environment;

operating the EM source to irradiate the reagent deposit by way of a path directed through the transparent substrate; and

,

utilizing the pyroelectric transducer to detect the energy absorbed by the reagent deposit upon irradiation by the EM source and generate output signals corresponding with a measure of the detected energy, such that the output signal corresponds with the degree of exposure of the target analyte to the reagent deposit;

wherein the reagent deposit is spaced apart from the pyroelectric transducer.

2. The method of claim 1, wherein said step of positioning the pyroelectric transducer includes positioning the pyroelectric transducer intermediate the transparent substrate and the EM source, such that during the step of operating the EM source, the EM source irradiates the reagent deposit by way of a path through the pyroelectric film and through the transparent substrate.

3. The method of claim 1, further comprising the step of positioning the transparent substrate intermediate the pyroelectric transducer and EM source, prior to the step of directing the sample environment past the reagent deposit, thereby initiating a reaction between the reagent deposit and any target analyte in the sample environment.

4. The method of claim 1, further comprising the step of positioning a second EM source proximal the reagent deposit such that the first surface is positioned intermediate the second surface and the second EM source.

5. The method of claim 1, further comprising the step of providing a second substrate that supports a second reagent deposit on a first surface of the second substrate and spaced apart from the pyroelectric transducer such that the second reagent deposit faces the pyroelectric transducer, wherein said step of operating the EM source irradiates the second reagent deposit by way of a path directed through the transparent substrate and through the pyroelectric transducer.

6. The method of claim 1, wherein said step of positioning the pyroelectric transducer includes positioning the pyroelectric transducer intermediate the transparent substrate and a second EM source, wherein said pyroelectric transducer is substantially transparent, such that, in the step of operating either EM source, the reagent deposit is irradiated by way of a path directed through the pyroelectric transducer.

7. The method of claim 1, wherein the reagent deposit is supported on the transparent substrate such that the reagent deposit faces and is spaced apart from the pyroelectric transducer, and wherein the transparent substrate and the pyroelectric transducers are spaced apart, such that, in the step of directing a sample environment past the reagent deposit, thereby initiating a reaction between the reagent deposit and any target analyte in the sample environment, a flow stream of the sample environment is directed between the pyroelectric transducer and the transparent substrate.

8. The method of claim 7, wherein the EM source is a light emitting source such that, in the step of utilizing the pyroelectric transducer, the pyroelectric transducer detects the amount of heat absorbed in the reagent deposit upon illumination by the light emitting source.

9. The method of claim 1, further comprising the step of positioning a reflective surface relative to the EM source such that the transparent substrate, the reagent deposit, and the pyroelectric transducer are positioned intermediate the reflective surface and the EM source, and wherein, the step of operating the EM source, directs energy to the reflective surface and the reflective surface redirects the energy to the reagent deposit.

10. The method of claim 1, further comprising selecting the reagent deposit from a group of one or more of a colorimetric reagent deposit, florescent reagent deposit and luminescent reagent deposit such that in the step of directing the sample environment past the reagent deposit, thereby initiating a reaction between the reagent deposit and any target analyte in the sample environment, the reagent deposit changes a light reflecting characteristic upon exposure to the target analyte.