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Methods and arrays for detecting biological molecules

  • US 7,429,466 B2
  • Filed: 06/18/2002
  • Issued: 09/30/2008
  • Est. Priority Date: 01/24/2000
  • Status: Expired due to Term
First Claim
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1. A method for staining cells to detect one or more biological molecules in said cells, comprising the steps of:

  • immobilizing a plurality of antibodies on a first support, wherein each of said antibodies is provided at predetermined positions on said first support such that each of said antibodies can be identified by the position where it is immobilized;

    (b) placing said cells on a second support;

    (c) fixing said cells with a fixation solution;

    (d) contacting said antibodies on said first support with said cells on said second support to allow said antibodies to bind to any corresponding interacting biological molecules in said cells;

    (e) separating said first support from said second support whereby one or more of said bound antibodies remain bound to the corresponding biological molecules after separation;

    (f) detecting said biological molecules by detecting said antibodies bound to said biological molecules in said cells.

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