Methods and arrays for detecting biological molecules
First Claim
1. A method for staining cells to detect one or more biological molecules in said cells, comprising the steps of:
- immobilizing a plurality of antibodies on a first support, wherein each of said antibodies is provided at predetermined positions on said first support such that each of said antibodies can be identified by the position where it is immobilized;
(b) placing said cells on a second support;
(c) fixing said cells with a fixation solution;
(d) contacting said antibodies on said first support with said cells on said second support to allow said antibodies to bind to any corresponding interacting biological molecules in said cells;
(e) separating said first support from said second support whereby one or more of said bound antibodies remain bound to the corresponding biological molecules after separation;
(f) detecting said biological molecules by detecting said antibodies bound to said biological molecules in said cells.
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Abstract
Arrays and methods for detecting one or more biological molecules, where the methods generally comprise the steps of: providing a first support immobilized with one or more reagents; providing a second support immobilized with one or more of ligands; contacting the reagents immobilized to the first support with the ligands immobilized on the second support whereby one or more of the reagents bind to one or more of the ligands; and separating the first support from the second support so that one or more of the bound reagents remain bound to one or more ligands on the second support after separation. In one preferred method, proteins are immobilized on a support with adequate strength so that the proteins can be dissociated from the support under certain conditions, such as after binding with other proteins immobilized on another support. For example, antibody arrays produced according to the present invention may be used to detect protein expressions in a protein lysate and may be used in immunostaining to reveal the presence and location of proteins in cells.
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Citations
21 Claims
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1. A method for staining cells to detect one or more biological molecules in said cells, comprising the steps of:
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immobilizing a plurality of antibodies on a first support, wherein each of said antibodies is provided at predetermined positions on said first support such that each of said antibodies can be identified by the position where it is immobilized; (b) placing said cells on a second support; (c) fixing said cells with a fixation solution; (d) contacting said antibodies on said first support with said cells on said second support to allow said antibodies to bind to any corresponding interacting biological molecules in said cells; (e) separating said first support from said second support whereby one or more of said bound antibodies remain bound to the corresponding biological molecules after separation; (f) detecting said biological molecules by detecting said antibodies bound to said biological molecules in said cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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Specification