Assay for human DNA for gender determination
First Claim
1. A primer pair for a sex determination of a human DNA sample, said primer pair selected from the group consisting of the following primer pairs:
- a primer pair comprising;
a forward primer having the sequence consisting of;
5′
-ACTGCAGTCCGCAGTCCGG-3′
(SEQ ID NO;
1); and
a reverse primer having the sequence consisting of;
5′
-CCACTGACATATTATCAGCCTTATTAC-3′
(SEQ ID NO;
2);
a primer pair comprising;
a forward primer having the sequence consisting of;
5′
-TGAAGAAATTCAGTTCATAGCTTGT-3′
(SEQ ID NO;
3); and
a reverse primer having the sequence consisting of;
5′
-CAGGAGATCCTGAGATTATGTGG-3′
(SEQ ID NO;
4); and
a primer pair comprising;
a forward primer consisting of;
5′
-AAACATGGCATGGGCCTCT-3′
(SEQ ID NO;
5); and
a reverse primer consisting of;
5′
-TTTTAAAGCCACAGACCTCTCTCT-3′
(SEQ ID NO;
6).
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Accused Products
Abstract
A method for determining gender from a human DNA sample. The loci of Alu element insertion is selected, amplified and evaluated in terms of size of the fragment. The gender assay utilizes AluSTXa for the X chromosome, AluSTYa for the Y chromosome, or both AluSTXa and AluSTYa, to reduce the possibility of error to a negligible quantity. The inserted chromosome yields a large fragment when the homologous region is amplified. The males are distinguished as having two DNA amplicons present, while females have only a single amplicon. The kit adapted for carrying out the method includes a pair of primers to amplify the locus and optionally polymerase chain reaction regents.
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Citations
12 Claims
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1. A primer pair for a sex determination of a human DNA sample, said primer pair selected from the group consisting of the following primer pairs:
-
a primer pair comprising;
a forward primer having the sequence consisting of;5′
-ACTGCAGTCCGCAGTCCGG-3′
(SEQ ID NO;
1); anda reverse primer having the sequence consisting of; 5′
-CCACTGACATATTATCAGCCTTATTAC-3′
(SEQ ID NO;
2);a primer pair comprising;
a forward primer having the sequence consisting of;5′
-TGAAGAAATTCAGTTCATAGCTTGT-3′
(SEQ ID NO;
3); anda reverse primer having the sequence consisting of; 5′
-CAGGAGATCCTGAGATTATGTGG-3′
(SEQ ID NO;
4); anda primer pair comprising;
a forward primer consisting of;5′
-AAACATGGCATGGGCCTCT-3′
(SEQ ID NO;
5); anda reverse primer consisting of; 5′
-TTTTAAAGCCACAGACCTCTCTCT-3′
(SEQ ID NO;
6). - View Dependent Claims (2, 3, 4)
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5. A kit for determining gender from a sample, comprising:
-
a pair of primers capable of amplifying a selected locus of a human DNA in an amplification reaction, said selected locus being in a non-combining X-Y homologous region and selected from the group consisting of a locus in AluSTYa on the Y chromosome and a locus in AluSTXa on the X chromosome, said region containing a monomorphic Alu insertion in one of the X chromosome and the Y chromosome, said pair of primers selected from the group consisting of the following primer pairs; a primer pair comprising a forward primer consisting of 5′
-ACTGCAGTCCGCAGTCCGG-3′
(SEQ ID NO;
1) and a reverse primer having the sequence consisting of 5′
-CCACTGACATATTATCAGCCTTATTAC-3′
(SEQ ID NO;
2);a primer pair comprising a forward primer consisting of 5′
-TGAAGAAATTCAGTTCATAGCTTGT-3′
(SEQ ID NO;
3) and a reverse primer consisting of 5′
- CAGGAGATCCTGAGATTATGTGG-3′
(SEQ ID NO;
4); anda primer pair comprising a forward primer having the sequence consisting of 5′
-AAACATGGCATGGGCCTCT-3′
(SEQ ID NO;
5) and a reverse primer having the sequence consisting of 5′
-TTTTAAAGCCACAGACCTCTCTCT-3′
(SEQ ID NO;
6); anda polymerase chain reaction reagent comprising a polymerase catalyzing the amplification of the selected locus. - View Dependent Claims (6, 7, 8, 9, 10, 11)
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12. A kit for determining gender from a sample, comprising:
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a pair of primers comprising a forward primer consisting of 5′
-AAACATGGCATGGGCCTCT-3′
(SEQ ID NO;
5) and a reverse primer consisting of 5′
-TTTAAAGCCACAGACCTCTCTCT-3′
(SEQ ID NO;
6); anda polymerase chain reaction reagent comprising a polymerase catalyzing the amplification of the alleles.
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Specification