Obtaining analyte information

• US 7,433,552 B2
• Filed: 12/22/2005
• Issued: 10/07/2008
• Est. Priority Date: 12/22/2005
• Status: Active Grant

• Current Assignee: Xerox Corporation (Xerox Holdings Corp.)
• Original Assignee: Palo Alto Research Center, Inc. (Xerox Holdings Corp.)
• Inventors: Kiesel, Peter, Schmidt, Oliver
• Primary Examiner(s): Healy; Brian
• Assistant Examiner(s): Anderson; Guy G
• Application Number:
  US11/316,303
• Publication Number:
  US 20070148760A1
• Time in Patent Office: 1,020 Days
• Field of Search: 435/287.2, 356/319, 356/451, 356/458, 356/519, 356/301, 422/58, 385/12, 250/458.1, 250/462.1, 436/164
• US Class Current: 385/12
• CPC Class Codes: G01J 1/42 using electric radiation detectors optical or mechanical part G01J1/04; by comparison with a reference light or electric value G01J1/10
• CPC Class Codes: G01J 3/02 Details
• CPC Class Codes: G01J 3/0208 using focussing or collimating elements, e.g. lenses or mirrors; performing aberration correction
• CPC Class Codes: G01J 3/0218 using optical fibers
• CPC Class Codes: G01J 3/0256 Compact construction
• CPC Class Codes: G01J 3/0262 Constructional arrangements for removing stray light
• CPC Class Codes: G01J 3/26 using multiple reflection, e.g. Fabry-Perot interferometer, variable interference filters
• CPC Class Codes: G01J 3/2803 using photoelectric array detector
• CPC Class Codes: G01N 21/4788 Diffraction for sizing particles G01N15/0205
• CPC Class Codes: G01N 21/78 producing a change of colour

Abstract

Light, such as from an analyte-wavelength converter or other optical sensor, is propagated to a detector or transmission structure with an entry surface and with output positions such as in an exit surface. For example, the position of light output by such a detector can be used to detect presence of an analyte such as a biomolecule or chemical. Or relative quantities of photons provided at positions of the exit surface can indicate analyte information such as presence, absence, quantity, or concentration. The detector or transmission structure can have a laterally varying energy transmission function, such as with a constant gradient or a step-like gradient. At the exit surface of the transmission structure, a photosensor array or position sensor can sense transmitted light or output photons, and, in response, circuitry can provide signals indicating the analyte information.

Claims

1. A method of detecting chemicals and biomolecules comprising the operations of:

generating light from a light source;

inputting the light into an optical sensor that outputs a narrow range of optical wavelengths when illuminated with a broad band of optical wavelengths; and

propagating the light output from the sensor to a detector with an entry surface that includes at least one layer with a laterally varying transmission property, and using the position of light output by the detector to detect the presence of an analyte.

2. The method of claim 1 in which the presence of the analyte changes over time;

the output light having a photon energy distribution that changes over time in accordance with the changing presence of the analyte over time;

the output light producing a photon energy

distribution on the detector'"'"'s entry surface that changes over time in accordance with the changing presence of the analyte over time;

the at least one layer having an exit surface including a set of two or more lateral positions and, between the entry and exit surfaces, extending in one or more lateral directions and transmitting photon energies from the entry surface to the exit surface as a function of lateral position in the exit surface, the function of lateral position varying in at least one of the lateral directions;

the act of propagating the light output and using the position of the light output comprising;

in response to the changing photon energy distribution on the entry surface, transmitting a changing distribution of photon energies through the at least one layer to the exit surface and providing, at the set of lateral positions, relative quantities of photons that change over time in accordance with the changing presence of the analyte over time; and

photosensing quantities of output photons provided at the set of lateral positions of the exit surface over time and using the photosensed quantities of two or more of the set of lateral positions to obtain one or more differential quantities that indicate the changing presence of the analyte over time.

3. The method of claim 2 in which the analyte is a biomolecule or a chemical.

4. The method of claim 2 in which the act of inputting the light comprises propagating the light through a lens.

5. The method of claim 2 in which the act of propagating the light output and using the position of the light output further comprises:

transmitting the light output through an optical fiber.

6. Apparatus comprising:

a light source that provides a broad band of optical wavelengths;

an optical sensor that outputs a narrow band of optical wavelengths when illuminated with the broad band of optical wavelengths from the light source; and

a detector that includes at least one layer with laterally varying transmission properties;

when the detector receives light output by the optical sensor, the at least one layer transmitting a portion of the received light at a position of the at least one layer, the detector using the position to detect presence of an analyte.

7. The apparatus of claim 6 in which the detector includes a graded Fabry-Perot cavity.

8. The apparatus of claim 6 in which the presence of the analyte changes over time;

the at least one layer has an entry surface and an exit surface, the exit surface including a set of two or more lateral positions and, between the entry and exit surfaces, extending in one or more lateral directions and transmitting photon energies from the entry surface to the exit surface as a function of lateral position in the exit surface, the function of lateral position varying in at least one of the lateral directions;

the output light having a distribution of photon energies that changes over time in accordance with the changing presence of the analyte over time;

the output light producing a photon energy distribution on the entry surface that changes over time in accordance with the changing presence of the analyte over time;

in response to the changing photon energy distribution on the entry surface the at least one layer transmitting a changing distribution of photon energies to the exit surface and providing, at the set of lateral positions of the exit surface, relative quantities of photons that change over time in accordance with the changing presence of the analyte over time;

the detector further including;

one or more photosensing components that sense quantities of photons from the set of lateral positions of the exit surface over time; and

a processor that uses sensed quantities of photons from two or more of the set of lateral positions to obtain one or more differential quantities that indicate the changing presence of the analyte over time.

9. The apparatus of claim 8 in which the optical sensor includes a photonic crystal.

10. The apparatus of claim 8 in which the at least one layer includes a graded Fabry-Perot cavity with two sets of distributed Bragg mirrors and, between them, a laterally graded cavity;

the light output by the optical sensor being spread across approximately an entire surface of one of the distributed Bragg mirrors.

11. The apparatus of claim 8 in which the one or more photosensing components include an IC with at least one of a photosensor array with CCD readout, a photosensor array with CMOS readout, and a position sensor.

12. A method comprising:

providing an analyte stimulus to an analyte-wavelength converter;

in response, the converter providing output light, the output light indicating analyte information;

propagating the output light to a transmission structure'"'"'s entry surface;

the transmission structure having an exit surface including a set of two or more positions;

the transmission structure being a layered structure with a laterally varying energy transmission function; and

transmitting the output light through the transmission structure to the exit surface so that relative quantities of photons are provided at the set of positions;

the relative quantities indicating the analyte information.

13. The method of claim 12, further comprising:

photosensing quantities of output photons provided at the set of the positions of the transmission structure'"'"'s exit surface and using the photosensed quantities to obtain analyte information.

14. The method of claim 13 in which the act of photosensing and using the photosensed quantities comprises:

using the photosensed quantities of two or more of the set of positions to obtain a differential quantity that indicates the analyte information.

15. Apparatus comprising:

an analyte-wavelength converter that responds to an analyte stimulus by providing output light indicating analyte information;

a transmission structure with entry and exit surfaces, the exit surface including a set of at least two positions;

the transmission structure being a layered structure with a laterally varying energy transmission function; and

a propagation component that propagates the output light from the converter to the transmission structure'"'"'s entry surface;

in response to the output light, the transmission

structure providing photons at the set of positions of the exit surface so that relative quantities of photons provided at the set of positions indicate the analyte information.

16. A system that includes the apparatus of claim 15, the system further comprising:

one or more photosensing components that sense quantities of photons from the set of positions of the exit surface; and

circuitry that responds to sensed quantities of photons from the set of positions by providing signals indicating the analyte information.

17. The system of claim 16 in which the circuitry includes a processor.

18. A method comprising:

providing an analyte stimulus to an analyte-wavelength converter;

in response, the converter providing output light, the output light having a distribution of photon energies that changes over time, indicating analyte information that changes over time;

propagating the output light to a transmission structure'"'"'s entry surface, the output light producing a photon energy distribution on the entry surface that changes over time in accordance with change in the analyte information over time;

the transmission structure having an exit surface including a set of two or more lateral positions;

the transmission structure including, between the entry and exit surfaces, a layered structure that extends in one or more lateral directions and that transmits photon energies from the entry surface to the exit surface as a function of lateral position in the exit surface, the function of lateral position varying in at least one of the lateral directions;

in response to the changing photon energy distribution on the entry surface, transmitting a changing distribution of photon energies through the transmission structure to the exit surface and providing, at the set of lateral positions, relative quantities of photons that change over time in accordance with the change in the analyte information over time; and

photosensing quantities of output photons provided at the set of lateral positions of the transmission structure'"'"'s exit surface over time and using the photosensed quantities of two or more of the set of lateral positions to obtain one or more differential quantities that indicate the change in the analyte information over time.

19. The method of claim 18 in which the act of providing the analyte stimulus comprises:

providing a fluid to a grating component that reflects a narrow band of photon energies that includes one of a set of peak values, the included peak value indicating the analyte information.

20. The method of claim 19 in which the set of peak values includes first and second peak values, the first peak value indicating an analyte is present in the fluid and the second peak value indicating the analyte is absent in the fluid.

21. The method of claim 18 in which the act of propagating the output light comprises at least one of transmitting, guiding, spreading, collimating, focusing, and imaging the output light.

22. The method of claim 18 in which the function of lateral position has a constant gradient or a step-like gradient in at least one of the lateral directions.

23. The method of claim 18 in which the set of lateral positions includes two positions;

the act of photosensing and using the photosensed quantities comprising comparing quantities sensed at the two positions.

24. A system comprising:

an analyte-wavelength converter that responds to an analyte stimulus by providing output light, the output light having a distribution of photon energies that changes over time, indicating analyte information that changes over time;

a transmission structure with entry and exit surfaces, the exit surface including a set of at least two lateral positions;

the transmission structure including, between the entry and exit surfaces, a layered structure that extends in one or more lateral directions and that transmits photon energies from the entry surface to the exit surface as a function of lateral position in the exit surface, the function of lateral position varying in at least one of the lateral directions;

a propagation component that propagates the output light from the converter to the transmission structure'"'"'s entry surface, the output light producing a photon energy distribution on the entry surface that changes over time in accordance with change in the analyte information over time;

in response to the changing photon energy distribution on the entry surface, the transmission structure transmitting a changing distribution of photon energies to the exit surface and providing, at the set of lateral positions of the exit surface, relative quantities of photons that change over time in accordance with the change in the analyte information over time;

one or more photosensing components that sense quantities of photons from the set of lateral positions of the exit surface over time; and

a processor that uses sensed quantities of photons from two or more of the set of lateral positions to obtain one or more differential quantities that indicate the change in the analyte information over time.

25. The apparatus of claim 24 in which the analyte-wavelength converter includes a grating component and a well that can receive fluid, the grating component providing the output light in response to fluid received in the well so that the received fluid is characterized by the analyte information indicated by the output light.

26. The apparatus of claim 25 in which the grating component provides the output light by reflecting a narrow band.

27. The apparatus of claim 24 in which the propagation component includes at least one of a surface;

a gap that includes air, gas, a transparent medium, or vacuum; and

a region of a lens.

28. A method of producing the apparatus of claim 24, the method comprising:

producing the apparatus so that the function of lateral position varies in one or more lateral directions.

29. The method of claim 28, further comprising:

producing the apparatus so that the propagation component spreads light from the converter across the transmission structure'"'"'s entry surface.