Label-free high-throughput optical technique for detecting biomolecular interactions

• US 7,435,385 B2
• Filed: 10/23/2001
• Issued: 10/14/2008
• Est. Priority Date: 10/30/2000
• Status: Active Grant

• Current Assignee: X-Body Incorporated (Bristol-Myers Squibb Company)
• Original Assignee: Sru Biosystems Incorporated
• Inventors: Lin, Bo, Li, Peter
• Primary Examiner(s): Warden; Jill
• Assistant Examiner(s): TURK, NEIL N
• Application Number:
  US10/415,037
• Publication Number:
  US 20040132172A1
• Time in Patent Office: 2,548 Days
• Field of Search: None
• US Class Current: 422/82.05
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/4788 Diffraction for sizing particles G01N15/0205
• CPC Class Codes: G01N 21/7743 the reagent-coated grating coupling light in or out of the waveguide
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G02B 5/1809 with pitch less than or comparable to the wavelength
• CPC Class Codes: Y10T 436/24 Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

Abstract

Methods and compositions are provided for detecting biomolecular interactions. The use of labels is not required and the methods can be performed in a high-throughput manner. The invention also provides optical devices useful as narrow band filters.

Claims

1. A method of analyzing or identifying one or more molecules comprising:

(a) contacting a sample comprising the one or more molecules with a colorimetric resonant reflectance optical sensor such that one or more of the one or more molecules become immobilized to the colorimetric resonant reflectance optical sensor;

(b) detecting the one or more molecules immobilized to the colorimetric resonant reflectance optical sensor;

(c) eluting the immobilized one or more molecules from the colorimetric resonant reflectance optical sensor; and

(d) subjecting the one or more molecules to mass spectrometry analysis, wherein one or more molecules are analyzed or identified.

2. The method of claim 1, wherein the one or more molecules immobilized to the colorimetric resonant reflectance optical sensor are quantified.

3. The method of claim 1, wherein the one or more molecules immobilized to the colorimetric resonant reflectance optical sensor are detected by determining a first peak wavelength of the colorimetric resonant reflectance optical sensor prior to contacting the sample with the colorimetric resonant reflectance optical sensor;

determining a second peak wavelength after contacting the sample with the colorimetric resonant reflectance optical sensor and comparing the first and second peak wavelengths, wherein a shift in peak wavelength indicates that the one or more molecules of the sample have immobilized to the colorimetric resonant reflectance optical sensor.

4. The method of claim 1, wherein analyzing or identifying the one or more molecules immobilized to the colorimetric resonant reflectance optical sensor comprises the use of a tag.

5. The method of claim 4, wherein the tag is biotin, succinimidyl-6-[a-methyl-a-(2-pyridyl-dithio) toluamido] hexanoate (SMPT), dimethylpimelimidate (DMP), or histidine.

6. The method of claim 1, wherein the one or more molecules are immobilized to the colorimetric resonant reflectance optical sensor by one or more specific binding substances on the surface of the colorimetric resonant reflectance optical sensor.

7. The method claim 6, wherein the one or more specific binding substances comprise a nucleic acid, polypeptide, antigen, polyclonal antibody, monoclonal antibody, single chain antibody (scFv), F(ab) fragment, F(ab′

)₂ fragment, Fv fragment, small organic molecule, cell, virus, bacteria, biological sample or a combination thereof.

8. The method of claim 1, wherein the colorimetric resonant reflectance optical sensor is coupled to a microfluidic channel.