Scaffold for culturing cells

• US 7,435,594 B2
• Filed: 02/04/2005
• Issued: 10/14/2008
• Est. Priority Date: 06/30/2004
• Status: Expired due to Fees

• Current Assignee: Hokkaido University
• Original Assignee: Hokkaido University
• Inventors: Osada, Yoshihito, Gong, Jian Ping, Chen, Yongmei
• Primary Examiner(s): Naff; David M
• Application Number:
  US11/050,426
• Publication Number:
  US 20060003442A1
• Time in Patent Office: 1,348 Days
• Field of Search: None
• US Class Current: 435/397
• CPC Class Codes: C12M 25/14 Scaffolds; Matrices in general C12N5/0068
• CPC Class Codes: C12N 2533/30 Synthetic polymers thermoreactive polymers, e.g. PNIPAm, C12N2539/10
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06

Abstract

A cell culture scaffold containing a gel having a network structure comprised of a synthetic polymer such that cultured cells spread in a shorter time and the number of adsorbed cultured cells per unit area is larger than in the case of using a gel having a network structure comprised of polyacrylic acid, while taking advantage of synthetic polymers with low manufacturing cost, easy quality control and no risk of virus infection in cultured cell. Used as the cell culture scaffold is a gel containing asynthetic polymer obtained by polymerization or copolymerization of a monomer having a sulfonic group such as p-styrenesulfonic acid sodium salt (NaSS) and 2-acrylamide-2-methylpropane sulfonic acid sodium salt (NaAMPS).

Claims

1. A scaffold for culturing cells containing a gel comprising a synthetic polymer obtained by copolymerization of p-styrenesulfonic acid alkali metal salt and dimethyl acrylamide, wherein the molar fraction of p-styrenesulfonic acid alkali metal salt to the total of p-styrenesulfonic acid alkali metal salt and dimethyl acrylamide is 6 mol % or more.

7. A method for culturing cells including the step of culturing cells on the scaffold according to claim 1.

2. A scaffold for culturing cells containing a gel comprising a synthetic polymer obtained by copolymerization of p-styrenesulfonic acid alkali metal salt and dimethyl acrylamide, wherein the molar fraction of p-styrenesulfonic acid alkali metal salt to the total of p-styrenesulfonic acid alkali metal salt and dimethyl acrylamide is about 6 to 90 mol %.

3. A scaffold for culturing cells containing a gel comprising a synthetic polymer obtained by copolymerization of 2-acrylamide-2-methylpropane sulfonic acid alkali metal salt and dimethyl acrylamide, wherein the molar fraction of 2-acrylamide-2-methylpropane sulfonic acid alkali metal salt to the total of 2-acrylamide-2-methyipropane sulfonic acid alkali metal salt and dimethyl acrylamide is 30 mol % or more.

4. A scaffold for culturing cells containing a gel comprising a synthetic polymer obtained by copolymerization of 2-acrylamide-2-methylpropane sulfonic acid alkali metal salt and dimethyl acrylamide, wherein the molar fraction of 2-acrylamide-2-methyipropane sulfonic acid alkali metal salt to the total of 2-acrylamide-2-methylpropane sulfonic acid alkali metal salt and dimethyl acrylamide is about 30 to 70 mol %.

8. A method for culturing cells including the step of culturing cells on the scaffold according to claim 4.

5. A scaffold for culturing cells containing a gel comprising a synthetic polymer obtained by copolymerization of p-styrenesulfonic acid alkali metal salt, dimethyl acrylamide and crosslinking reagent, wherein:

the molar fraction of p-styrenesulfonic acid alkali metal salt to the total of p-styrenesulfonic acid alkali metal salt and dimethyl acrylamide is 6 mol % or more, and

the amount of crosslinking reagent to the total of p-styrenesulfonic acid alkali metal salt and dimethyl acrylamide is in a range of 0.1 to 10 mol %.

6. A scaffold for culturing cells containing a gel comprising a synthetic polymer obtained by copolymerization of 2-acrylamide-2-methylpropane sulfonic acid alkali metal salt, dimethyl acrylamide and crosslinking reagent, wherein:

the molar fraction of 2-acrylamide-2-methylpropane sulfonic acid alkali metal salt to the total of 2-acrylamide-2-methylpropane sulfonic acid alkali metal salt and dimethyl acrylamide is 30 mol % or more, and

the amount of crosslinking reagent to the total of 2-acrylamide-2-methylpropane sulfonic acid alkali metal salt and dimethyl acrylamide is in a range of 0.1 to 10 mol %.