Beads for capturing target cells from bodily fluid
First Claim
1. Beads for capturing target cells from a bodily fluid, which beads comprise:
- spheroids of a size between about 5 μ
m and about 500 μ
m, said spheroids having an exterior hydrophilic hydrogel surface that is constituted of an isocyanate- or a thiocyanate-functional polymer containing residues of PEG, PPG, or a copolymer thereof that are polymerized by urethane bonds, andagents which are directly or indirectly bound to said hydrogel via isocyanate or thiocyanate groups of said polymer, wherein said agents can contact and bind ligands on the surfaces of said target cells.
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Accused Products
Abstract
Spheroidal beads present an exterior surface of a hydrophilic hydrogel, which is an isocyanate-functional polymer that is polymerized by urethane bonds and cross-linked by urethane and urea bonds. Sequestering agents present at the surface are covalently bound to isocyanate groups or to intermediate linkers that are so bound. These beads allow sequestering agents to retain their native three-dimensional configuration, and as a result of such surface characteristics and hydrophilicity, they achieve highly effective capture of very small subpopulations of rare cells from bodily fluids or the like and very effectively deter nonspecific binding of other biomaterials present in such bodily fluid. They may be all-hydrogel spheroids or hydrogel-coated substrates.
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Citations
23 Claims
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1. Beads for capturing target cells from a bodily fluid, which beads comprise:
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spheroids of a size between about 5 μ
m and about 500 μ
m, said spheroids having an exterior hydrophilic hydrogel surface that is constituted of an isocyanate- or a thiocyanate-functional polymer containing residues of PEG, PPG, or a copolymer thereof that are polymerized by urethane bonds, andagents which are directly or indirectly bound to said hydrogel via isocyanate or thiocyanate groups of said polymer, wherein said agents can contact and bind ligands on the surfaces of said target cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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Specification