Microfluidic-based electrospray source for analytical devices with a rotary fluid flow channel for sample preparation
First Claim
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1. A method for providing a nanoliter volume of a fluid sample from a microfluidic device into an analytical device, the method comprising the steps of:
- providing the analytical device;
providing the microfluidic device, wherein the microfluidic device comprises;
a first elastomeric layer having a fluid flow channel disposed therein,a second elastomeric layer positioned in contact with the first elastomeric layer, wherein the second elastomeric layer has an integrated microfluidic pump disposed therein, and wherein the pump controls the flow of fluid through the fluid flow channel,a sample preparation chamber coupled to the fluid flow channel, the sample preparation chamber comprising a rotary fluid flow channel, anda capillary having at least a portion fitted within the fluid flow channel, wherein the capillary comprises a nozzle, and wherein the capillary is interconnected to the analytical device for introducing the fluid sample from the fluid flow channel to the analytical device; and
injecting at least a portion of the fluid sample from the microfluidic device directly into the analytical device through the capillary nozzle.
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Abstract
This invention provides microfluidic devices and methods for using the same. Microfluidic devices of the present invention comprises a first elastic layer, a fluid flow channel within the elastic layer; and a means for providing a fluid sample from the fluid flow channel to an analytical device. The present invention also provides an analytical apparatus comprising such a microfluidic device and an analytical device.
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Citations
25 Claims
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1. A method for providing a nanoliter volume of a fluid sample from a microfluidic device into an analytical device, the method comprising the steps of:
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providing the analytical device; providing the microfluidic device, wherein the microfluidic device comprises; a first elastomeric layer having a fluid flow channel disposed therein, a second elastomeric layer positioned in contact with the first elastomeric layer, wherein the second elastomeric layer has an integrated microfluidic pump disposed therein, and wherein the pump controls the flow of fluid through the fluid flow channel, a sample preparation chamber coupled to the fluid flow channel, the sample preparation chamber comprising a rotary fluid flow channel, and a capillary having at least a portion fitted within the fluid flow channel, wherein the capillary comprises a nozzle, and wherein the capillary is interconnected to the analytical device for introducing the fluid sample from the fluid flow channel to the analytical device; and injecting at least a portion of the fluid sample from the microfluidic device directly into the analytical device through the capillary nozzle. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method for providing a nanoliter volume of a fluid sample from a microfluidic device into an analytical device, the method comprising the steps of:
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providing the analytical device; providing the microfluidic device, wherein the microfluidic device comprises; a first elastomeric layer having a fluid flow channel disposed therein, a second elastomeric layer having a pressure channel disposed therein, wherein the pressure channel controls the flow of fluid through said fluid flow channel, a sample preparation chamber coupled to the fluid flow channel, the sample preparation chamber comprising a rotary fluid flow channel, and a capillary having at least a portion fitted within the fluid flow channel, wherein the capillary comprises a nozzle, and wherein the capillary is interconnected to the analytical device for introducing the fluid sample from the fluid flow channel to the analytical device; and injecting at least a portion of the fluid sample from the microfluidic device to the analytical device through the capillary nozzle. - View Dependent Claims (12, 13, 14, 15)
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16. A system to provide a nanoliter volume of a non-aqueous fluid sample from a microfluidic device to an analytical device, the system comprising:
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an analytical device for analyzing at least a portion of the non-aqueous fluid sample; and a microfluidic device for providing the at least portion of the fluid sample to the analytical device, wherein the microfluidic device comprises; a first elastomeric layer having a fluid flow channel disposed therein, a second elastomeric layer positioned in contact with the first elastomeric layer, wherein the second elastomeric layer has an integrated microfluidic pump disposed therein, and wherein the pump controls the flow of fluid through the fluid flow channel, a sample preparation chamber coupled to the fluid flow channel, the sample preparation chamber comprising a rotary fluid flow channel, and a capillary having at least a portion fitted within the fluid flow channel, wherein the capillary comprises a nozzle, and wherein the capillary is interconnected to the analytical device for introducing the fluid sample from the fluid flow channel to the analytical device. - View Dependent Claims (17, 18, 19, 20, 21)
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22. A system to provide a nanoliter volume of a non-aqueous fluid sample from a microfluidic device to an analytical device, the system comprising:
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an analytical device for analyzing at least a portion of the non-aqueous fluid sample; and a microfluidic device for providing the at least portion of the fluid sample to the analytical device, wherein the microfluidic device comprises; a first elastomeric layer having a fluid flow channel disposed therein, a second elastomeric layer positioned in contact with the first elastomeric layer, wherein the second elastomeric layer has an integrated microfluidic pump disposed therein, and wherein the pump controls the flow of fluid through the fluid flow channel, and a capillary having at least a portion fitted within the fluid flow channel, wherein the capillary comprises a nozzle, and wherein the capillary is interconnected to the analytical device for introducing the fluid sample from the fluid flow channel to the analytical device; and a sample preparation chamber in fluid communication with the fluid flow channel of the microfluidic device, wherein the sample preparation chamber supplies an analyte to the non-aqueous fluid sample that is analyzed by the analytical device, and wherein the sample preparation chamber comprises a rotary fluid flow channel. - View Dependent Claims (23, 24, 25)
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Specification