Phytase enzymes, nucleic acid sequences encoding phytase enzymes and vectors and host cells incorporating same

• US 7,449,317 B2
• Filed: 01/26/2007
• Issued: 11/11/2008
• Est. Priority Date: 10/26/2001
• Status: Expired due to Fees

• Current Assignee: Genencor International Incorporated (International Flavors & Fragrances Incorporated)
• Original Assignee: Genencor International Incorporated (International Flavors & Fragrances Incorporated)
• Inventors: Goedegebuur, Frits, Power, Scott D., Bower, Benjamin, Clarkson, Kathleen A.
• Primary Examiner(s): Saidha; Tekchand
• Application Number:
  US11/698,523
• Publication Number:
  US 20070207525A1
• Time in Patent Office: 655 Days
• Field of Search: 436/196, 436/252.3, 436/320.1, 536/23.2
• US Class Current: 435/196
• CPC Class Codes: C12N 9/16 acting on ester bonds (3.1)

Abstract

DNA is provided which encodes an enzyme having phytase activity isolated from Penicillium, Fusarium, Humicola and Emericella. Also provided for is a method of isolating DNA encoding an enzyme having phytase activity from organisms which possess such DNA, transformation of the DNA into a suitable host organism, expression of the transformed DNA and the use of the expressed phytase protein in feed as a supplement.

Claims

1. An isolated polynucleotide encoding an enzyme, said enzyme having phytase activity and comprising an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO:

8.

3. An expression construct comprising the polynucleotide of claim 1.

4. A vector comprising the expression construct of claim 3.

5. A host cell transformed with the vector of claim 4.

6. A method of producing an enzyme having phytase activity, comprising:

(a) providing a host cell transformed with an expression vector comprising the polynucleotide of in claim 1;

(b) cultivating said transformed host cell under conditions suitable for said host cell to produce said phytase; and

(c) recovering said phytase.

7. The method of claim 6, wherein said host cell is an Aspergillus species.

2. An isolated polynucleotide encoding an enzyme having phytase activity, comprising a polynucleotide sequence (i) having at least 95% identity to the nucleotide sequence of SEQ ID NO:

7, or (ii) the full complement of the sequence of (i).

8. An expression construct comprising the polynucleotide of claim 2.

9. A vector comprising the expression construct of claim 8.

10. A host cell transformed with the vector of claim 9.

11. A method of producing an enzyme having phytase activity, comprising:

(a) providing a host cell transformed with an expression vector comprising the polynucleotide of claim 2;

(b) cultivating said transformed host cell under conditions suitable for said host cell to produce said phytase; and

(c) recovering said phytase.

12. The method of claim 11, wherein said host cell is an Aspergillus species.