System for simple nucleic acid analysis
First Claim
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1. An apparatus for detecting nucleic acids in a sample, comprising:
- (a) a binding space for purifying the nucleic acids by immobilizing the nucleic acids and separating impurities,(b) an amplification space for amplifying the nucleic acids wherein at least a part of the amplification space is identical to a part of the binding space, and(c) a detection space for detecting the nucleic acids.
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Abstract
A system for simple nucleic acid analysis is described in which the amplification space contains at least a part of the binding space. An additional improvement is achieved by the design of the binding or/and amplification space which is optionally surrounded by a heatable metal layer.
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Citations
16 Claims
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1. An apparatus for detecting nucleic acids in a sample, comprising:
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(a) a binding space for purifying the nucleic acids by immobilizing the nucleic acids and separating impurities, (b) an amplification space for amplifying the nucleic acids wherein at least a part of the amplification space is identical to a part of the binding space, and (c) a detection space for detecting the nucleic acids. - View Dependent Claims (2, 3, 4, 5, 6, 8, 9, 10, 14, 15, 16)
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7. An apparatus for amplifying nucleic acids comprising a capillary reaction vessel surrounded by a single heatable metal layer wherein the layer is coated on the capillary reaction vessel.
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11. An apparatus for detecting nucleic acids in a liquid sample, comprising:
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(a) a space comprising a capillary reaction vessel surrounded by a heatable metal layer, wherein the interior surface of the vessel binds nucleic acids; (b) reagents for amplifying and detecting the nucleic acids that become bound to the surface; (c) a sample transport mechanism which transports the sample and reagents through the space. - View Dependent Claims (12, 13)
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Specification