Methods and compositions for tissue regeneration
First Claim
1. A cell preparation for tissue regeneration comprisinga) a first component comprising an extracellular matrix or matrix material containing fibrinogen;
- andb) a second component comprising from about 1 ×
103 cells/μ
l to about 50 ×
103 cells/μ
l and thrombin, wherein the cells comprise one or more keratinocytes and fibroblasts that secrete one or more biologically active molecules selected from the group consisting of GM-CSF, VEGF, KGF, bFGF, TGFβ
, angiopoietin, EGF, IL-Iβ
, IL-6, IL-8, TGFα
, and TNFα
,wherein the one or more keratinocytes and fibroblasts are mitotically inactivated allogeneic cells.
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Accused Products
Abstract
The present invention provides the use and composition of matter of angiogenic or other growth/cytokine factors expressed by mixtures of allogeneic human cell strains or lines of various types and stages of differentiation. Also provided are unencapsulated preparations (mixed with or applied to extracellular matrix material or synthetic biocompatible substances) for the purpose of temporary application to wounds or defects in the skin or other tissues for the restoration of blood supplying connective tissue to enable organ-specific cells to reestablish organ integrity as well as to inhibit excessive scar formation.
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Citations
34 Claims
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1. A cell preparation for tissue regeneration comprising
a) a first component comprising an extracellular matrix or matrix material containing fibrinogen; - and
b) a second component comprising from about 1 ×
103 cells/μ
l to about 50 ×
103 cells/μ
l and thrombin, wherein the cells comprise one or more keratinocytes and fibroblasts that secrete one or more biologically active molecules selected from the group consisting of GM-CSF, VEGF, KGF, bFGF, TGFβ
, angiopoietin, EGF, IL-Iβ
, IL-6, IL-8, TGFα
, and TNFα
,wherein the one or more keratinocytes and fibroblasts are mitotically inactivated allogeneic cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
- and
Specification