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Medium containing pipecholic acid and gamma amino butyric acid and culture of embryonic stem cells

  • US 7,449,334 B2
  • Filed: 09/08/2005
  • Issued: 11/11/2008
  • Est. Priority Date: 09/08/2004
  • Status: Active Grant
First Claim
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1. A method for culturing human stem cells in an undifferentiated state on a matrix without the need for feeder cells or conditioned medium, the method comprising the step of:

  • culturing human embryonic stem cells on a matrix that comprises a solubulized basement membrane preparation extracted from the Engelbreth-Holm-Swarm mouse sarcoma or a matrix that comprises human matrix proteins collagen IV, fibronectin, laminin, and vitronectin in a medium absent feeder cells and conditioned media, the medium comprising salts, vitamins, amino acids, glucose, a fibroblast growth factor, gamma amino butyric acid, pipecholic acid, lithium and transforming growth factor beta, in sufficient amounts to maintain the human stem cells in an undifferentiated state through multiple successive culture passages.

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